1,720,956 research outputs found
Endometrial CD16(+) and CD16(-) NK cell count in fertility and unexplained infertility
Get PDFProblem: Peripheral counts of CD16+ NK cells have been well characterized in reproductive failure. However, not enough case-control clinical studies have been conducted to establish normal or abnormal CD16+/- values in the endometrium. Method of Study: Peripheral and endometrial NK cell counts by FACS, IL-6 and VEGF cytokines levels by ELISA were characterized in fertile women and unexplained infertility patients with implantation failures (UI-IF) during implantation window. ROC and correlation analysis were performed. Results: ROC analysis revealed endometrial CD16+ NK cells, IL-6 and VEGF as good diagnostic parameters for unexplained infertility. Almost half of UI-FI patients showed increased total and CD16+ NK cell counts correlating with decreased levels of endometrial IL-6 and VEGF. No correlation was found with peripheral blood values. Conclusions: Increased CD16+ NK cells were associated to IL-6 and VEGF deficiency in a high proportion of UI-IF patients. Testing for these immunomarkers could be a potential tool in infertility diagnosis.Fil: Junovich, Gisela Yael. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Azpiroz, Maria Agustina. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Incera, Maria Eugenia. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrer, Maria Constanza. Centro de Investigación de la Fundación Repro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pasqualini, Rodolfo Agustin. Halitus Instituto Medico; ArgentinaFil: Gutierrez, Gabriela Lidia. Centro de Investigación de la Fundación Repro; Argentina. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Potential biomarkers of infertility associated with microbiome imbalances
No full textProblem: The aim of this study was to investigate the possible relationship between vaginal/rectal microbiome disbalances and miRNA expression with infertility. Method of study: Observational, exploratory, preliminary study. A total of 287 multiple IVF failure infertile patients were recruited. Twenty fertile women, not IVF failure, were recruited as the control group. Swab samples were collected from the vagina and rectum. Microbial composition by NGS and miRNA expression by real-time PCR of vaginal and rectal samples was measured. Immunometabolic markers from blood (insulin, vitamin D, LDL-cholesterol, ANA, TPO, Tg, and ASCA antibodies) and saliva (sIgA) were analyzed. Result(s): Infertile patients showed a lower bacterial richness and increased Firmicutes/Bacteroidetes ratio at rectal level and an increased Lactobacillus brevis/Lactobacillus iners ratio in vaginal samples regarding the fertile group. In the same rectal swab samples, we found that miR-21-5p, which is associated with tight junction disruption and yeast overgrowth, is upregulated and that miR-155-5p, which is associated with inflammation, is overexpressed in the unexplained infertile group (*p <.05). These deregulated miRNAs were also upregulated in the vaginal samples from the same patients (*p <.05). Conclusion: miRNAs could be potential biomarkers of the inflammatory impact of microbiome disbalances in unexplained infertile women.Fil: Azpiroz, Maria Agustina. Microgenesis Co; Argentina. Abm Laboratorios Tandil; ArgentinaFil: Orguilia, Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Inmunogenesis; ArgentinaFil: Palacio, Maria Ines. Abm Laboratorios Tandil; ArgentinaFil: Malpartida, Alejandro Rodolfo. Microgenesis Co; ArgentinaFil: Mayol, Soledad. Inmunogenesis; ArgentinaFil: Mor, Gil. Wayne State University (wayne State University); Estados UnidosFil: Gutierrez, Gabriela Lidia. Microgenesis Co; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Expresión de MICA en el endometrio de mujeres infértiles
Get PDFLas células natural killer (NK) constituyen la principal población leucocitaria en el endometrio durante la implantación y el embarazo temprano. Existen dos subpoblaciones, la CD16+ o citotóxica, y la CD16- o angiogénica. Al momento de la implantación embrionaria debe existir un equilibrio entre ambos recuentos a favor de la vascularización endometrial. Las células NK expresan receptores que al unirse a la célula blanco desencadenan la actividad citolítica o producción de citoquinas inflamatorias (como IFNγ) o angiogénicas (VEGF). El receptor de activación NKG2D es capaz de unirse a MICA expresada en varios tejidos, incluyendo el endometrio. A pesar de su importancia, existe poca evidencia del rol de MICA en el campo de la reproducción. A partir de estos antecedentes, decidimos caracterizar los niveles endometriales de ARNm de MICA, IFNγ y VEGF para su posible aplicación como parámetros inmunológicos en el estudio de patologías reproductivas: aborto a repetición y fallas de implantación embrionaria. Los resultados obtenidos demuestran que mujeres abortadoras presentan un porcentaje de células NK endometriales y recuento de células NK angiogénicas significativamente menor respecto a las mujeres fértiles, existiendo una correlación positiva con la expresión de VEGF. Estas pacientes presentan, además, niveles significativamente aumentados de ARNm de MICA. Si bien no existen diferencias en los niveles de IFNγ, encontramos una correlación positiva significativa entre IFNγ y MICA. Las pacientes con fallas de implantación no han mostrado valores alterados de MICA por lo que este parámetro podría ser considerado un estudio diagnóstico diferencial entre ambas patologías reproductivas.Fil: Ferrer, Maria Constanza. Centro de Investigación de la Fundación Repro; Argentina. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Azpiroz, Maria Agustina. Halitus Instituto Medico; ArgentinaFil: Junovich, Gisela Yael. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Incera, Maria Eugenia. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Iglesias, Ana. Halitus Instituto Medico; ArgentinaFil: Pappalardo, Cecilia. Halitus Instituto Medico; ArgentinaFil: Pasqualini, Agustina. Halitus Instituto Medico; ArgentinaFil: Gutierrez, Gabriela Lidia. Centro de Investigación de la Fundación Repro; Argentina. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Expresión de MICA en el endometrio de mujeres infértiles
Get PDFLas células natural killer (NK) constituyen la principal población leucocitaria en el endometrio durante la implantación y el embarazo temprano. Existen dos subpoblaciones, la CD16+ o citotóxica, y la CD16- o angiogénica. Al momento de la implantación embrionaria debe existir un equilibrio entre ambos recuentos a favor de la vascularización endometrial. Las células NK expresan receptores que al unirse a la célula blanco desencadenan la actividad citolítica o producción de citoquinas inflamatorias (como IFNγ) o angiogénicas (VEGF). El receptor de activación NKG2D es capaz de unirse a MICA expresada en varios tejidos, incluyendo el endometrio. A pesar de su importancia, existe poca evidencia del rol de MICA en el campo de la reproducción. A partir de estos antecedentes, decidimos caracterizar los niveles endometriales de ARNm de MICA, IFNγ y VEGF para su posible aplicación como parámetros inmunológicos en el estudio de patologías reproductivas: aborto a repetición y fallas de implantación embrionaria. Los resultados obtenidos demuestran que mujeres abortadoras presentan un porcentaje de células NK endometriales y recuento de células NK angiogénicas significativamente menor respecto a las mujeres fértiles, existiendo una correlación positiva con la expresión de VEGF. Estas pacientes presentan, además, niveles significativamente aumentados de ARNm de MICA. Si bien no existen diferencias en los niveles de IFNγ, encontramos una correlación positiva significativa entre IFNγ y MICA. Las pacientes con fallas de implantación no han mostrado valores alterados de MICA por lo que este parámetro podría ser considerado un estudio diagnóstico diferencial entre ambas patologías reproductivas.Fil: Ferrer, Maria Constanza. Centro de Investigación de la Fundación Repro; Argentina. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Azpiroz, Maria Agustina. Halitus Instituto Medico; ArgentinaFil: Junovich, Gisela Yael. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Incera, Maria Eugenia. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Iglesias, Ana. Halitus Instituto Medico; ArgentinaFil: Pappalardo, Cecilia. Halitus Instituto Medico; ArgentinaFil: Pasqualini, Agustina. Halitus Instituto Medico; ArgentinaFil: Gutierrez, Gabriela Lidia. Centro de Investigación de la Fundación Repro; Argentina. Halitus Instituto Medico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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