40 research outputs found
Ce<sub>2</sub>O<sub>3</sub> nanoparticle synthesis, characterization, and application to callus formation and plant regeneration from mature embryo culture of wheat (<i>Triticum aestivum</i> L.)
No full textCerium oxide nanoparticles (Ce2O3-NPs) are widely used for their catalytic and oxidative properties, such as in diesel additives and potential oncology treatments. However, limited data exists on their impact on callus formation and plant regeneration in wheat. This study investigates the effects of different genotypes and Ce2O3-NPs concentrations on callus formation and plant regeneration in wheat (Triticum aestivum L.) using mature embryo culture. Our hypothesis was that Ce2O3-NPs would enhance callus and plant regeneration rates in a genotype-dependent manner. We used Murashige and Skoog (MS) medium with varying concentrations of Ce2O3-NPs (1-7 mg/L) to assess their effects. The K & imath;rik genotype showed the highest callus formation (7 mg/L Ce2O3-NPs), while the Rumeli genotype showed the lowest (1 mg/L Ce2O3-NPs). Embryogenic callus formation was the highest in K & imath;rik without Ce2O3-NPs and the lowest in Rumeli with 3 mg/L Ce2O3-NPs. Root and shoot formation rates in Rumeli were 16.03% and 51.40%, respectively, compared to 3.94% and 40.49% in K & imath;rik. The main outcomes demonstrated that 1 mg/L Ce2O3-NPs promoted root formation, while 7 mg/L was optimal for shoot formation. Genotypic effects on callus, root, and shoot formation, as well as plant regeneration, were significant. MS media with Ce2O3-NPs enhanced callus formation and regeneration in wheat tissue culture, highlighting the importance of genotype in these processes. Future research should explore the underlying mechanisms of Ce2O3-NPs' effects on plant tissue culture and extend the study to other plant species to validate these findings
Highly sensitive glucose sensor based on ZnO NPs as a biomimetic enzyme
No full textResearch Development Center of Ataturk UniversityAtaturk University; Ataturk University, TurkeyAtaturk University; [FAD-2018-6321]This research has been performed under the project numbered FAD-2018-6321 and supported by the Research Development Center of Ataturk University. The authors acknowledged the support of Ataturk University, Turkey, for this work. The authors, Hayrunnisa Nadaroglu and Azize Alayli Gungor, hereby confirm that this manuscript is performed according to guidelines and has not already been published nor is it under consideration for publication elsewhere. This article does not contain any studies with human or animal subjects.In this work, the development of zinc biosensors based on zinc nanoparticles (Zn NPs) biomimetic enzymes were carried out to spectrophotometrically detect glucose in food samples. Briefly; Glucose oxidase enzyme reacts with glucose in food samples to form gluconic acid and H2O2. Behind; the peroxide formed can be measured spectrophotometrically using ABTS substrate with the enzyme mimic effect of Zn NPs. In this way, the amount of glucose was determined by a simple, effective and selective method by measuring the change in absorbance of the reaction medium, which changed by oxidation. ZnO NPs were obtained from Zn(NO3)(2) by green synthesis method using raw fig extract. Then, the amount of glucose in some food samples was determined using ZnO NPs. The results showed that the glucose content in the samples was exactly and reproducibly measured with a correlation coefficient of 0.9812 at 3.47 mM-27.78 mM concentrations. This will greatly simplify the design and manufacture of the new biosensor, which can be used for glucose fixation in food samples in an inexpensive, efficient, environmentally friendly and fast manner
Eco-friendly synthesis of nano copper and its use in fenton-like reactions for methylene blue degradation
No full textCopper oxide nanoparticles (CuO NPs) were produced using green synthesis method with Cimin grape extract (Vitis vinifera cv.). The produced CuO NPs were used to remove methylene blue (MB) from water by degradation with Fentonlike reactions. The surface properties of the CuO NPs were determined by FT-IR, SEM and XRD techniques. Experimental parameters for MB removal were selected as: pH: 3 – 11; Temp: 20 - 80°C; initial MB concentration (15 -50 ), and CuO NPs concentrations (25 - 800 mg ). The best reaction conditions were found to be pH: 7 - 11, temperature: 40 - 45°C, interaction time: 120 min, initial MB concentration: 3.125 mg and CuO NPs concentration: 25 mg . Under these conditions, CuO NPs showed a 97.80% efficacy for the removal of MB from wastewater with Fentonlike process. Moreover, this study showed that reagents were reusable, inexpensive, biocompatible, easy to prepare, harmless and Fenton-like reaction conditions were created
Effects of Zinc, Copper and Iron Oxide Nanoparticles on Induced DNA Methylation, Genomic Instability and LTR Retrotransposon Polymorphism in Wheat (Triticum aestivum L.)
No full textNanomaterials with unique and diverse physico-chemical properties are used in plant science since they improve plant growth and development and offer protection against biotic and abiotic stressors. Previous studies have explored the effects of such nanomaterials on different plant mechanisms, but information about the effects of nanomaterials on induced DNA methylation, genomic instability and LTR retrotransposon polymorphism in wheat is lacking. Therefore, the present study highlights the key role of nanoparticles in DNA methylation and polymorphism in wheat by investigating the effects of ZnO, CuO and γ-Fe3O4 nanoparticles (NPs) on mature embryo cultures of wheat (Triticum aestivum L.). Nanoparticles were supplemented with Murashige and Skoog (MS) basal medium at normal (1X), double (2X) and triple (3X) concentrations. The findings revealed different responses to the polymorphism rate depending on the nanoparticle type and concentration. Genomic template stability (GTS) values were used to compare the changes encountered in iPBS profiles. ZnO, CuO and γ-Fe3O4 NPs increased the polymorphism rate and cytosine methylation compared to the positive control while reducing GTS values. Moreover, non-γ-Fe3O4 NPs treatments and 2X ZnO and CuO NP treatments yielded higher polymorphism percentages in both MspI- and HpaII-digested CRED-iPBS assays and were thus classified as hypermethylation when the average polymorphism percentage for MspI digestion was considered. On the other hand, the 3X concentrations of all nanoparticles decreased HpaII and MspI polymorphism percentages and were thus classified as hypomethylation. The findings revealed that MS medium supplemented with nanoparticles had epigenetic and genotoxic effects
SYNTHESIS, CHARACTERIZATION OF CuO NPs USING GREEN SYNTHESIS AND ITS APPLICATION AS A SELECTIVE NON-ENZYMATIC GLUCOSE BIOSENSOR
No full text
Esra Turgut, Sümeyra Gündüz, Hayrunnisa Nadaroğlu, Azize Alaylı, Kitosan Ayçiçeği ve Kitosan Ayçiçeği Nanodemir ile Atık Sulardan Metil Oranj Boyasının Uzaklaştırılması
No full textInvestigation of Antimicrobial and Genotoxic Effects of Fe2O3, NiO and CoO NPs Synthesized by Green Synthesis
No full textIt is seen that metal nanoparticles are used in many areas due to their antimicrobial effects. For this reason, our study focused on the production of alpha-Fe2O3, NiO and CoO NPs of golden nanoparticles, which are easily obtained with the use of Erzincan grape extract, safe to use, environmentally friendly and cost-effective. Metal ions synthesized by the green synthesis method were characterized using the Scanning Electron Microscope (SEM) analysis. From the SEM diagrams of the synthesized nanoparticles, it was determined that the nanoparticles were approximately 5 to 65 nm in size. Both antimicrobial, genotoxicity and cytotoxicity effects were investigated to determine the rates at which nanoparticles can be used as biosafe. Synthesized alpha-Fe2O3, NiO and CoO NPs showed excellent antibacterial properties on pathogen bacteria against human. In addition, it was determined that alpha-Fe2O3, NiO and CoO M-NPs showed genotoxic properties in parallel with increasing concentrations. This study, as far as we know, is the first report on microbial alpha-Fe2O3, NiO and CoO NPs and their biological properties synthesized by this statistical approach
