3 research outputs found
Correlation of Parasitaemia and Anemia in Mice Infected with Plasmodium berghei ANKA
No full textMalaria is an infectious disease caused by protozoa of the genus Plasmodium and transmitted through the bite of a female Anopheles mosquito. Plasmodium berghei ANKA is a species of rodent malaria parasite that is commonly used to study malaria pathology and the immune system against infections. Parasitaemia in malaria is the figure of malaria pathology due to some numbers of parasite-infected erythrocytes present in the peripheral blood. Hemoglobin (HGB) and hematocrit (HCT) levels are the parameters of anemia and some hematological changes caused by malaria infection. This study aimed to determine the correlation between parasitemia and anemia in BABL/c mice infected with Plasmodium berghei ANKA. Two uninfected and infected mice groups were compared for parasitemia, HGB, and HCT levels. Analysis statistics showed a significant difference in HGB and HCT between uninfected and infected groups. Pearson correlation analysis showed no significant correlation between parasitemia and HGB and HCT levels in infected mice. Anemia in mice infected with Plasmodium berghei ANKA can occur when parasitemia is even low; the higher parasitemia worsens the hamatological condition. Parasitemia plays a role independently in the severity of anemia. Plasmodium berghei infection in mice is useful for studying malaria anemia
Protein Encodes Plasmodium berghei Vaccine Candidates: A Review
No full textABSTRACT
Background: Malaria has become a serious threat in many developing countries, including Indonesia. Malaria infection without proper treatment can cause death. Plasmodium berghei (P. berghei) is a malaria parasite that mostly
infects rodents, and is often used as a model for in vivo antimalarial research in mice. Giving the right vaccine can
be one of the efforts to control and eliminate malaria.Objectives: The purpose of this article is to review the protein
coding for P. berghei as a vaccine candidate. Review: Antibodies are components of the immune system that play
a role in the humoral immune response. Expression of Pb51, P47, PbVaC, PSOP25 genes in P. berghei are selected
antigens against malaria parasites, has the ability to block malaria through chemotherapy and immunoprophylaxis
interventions efficiently as a potential malaria vaccine candidate. Conclusion: The protein coding for P. berghei
has good antigenicity as a candidates for malaria vaccine. The mechanism of blocking the transmission of parasites
to mosquitoes, induction of the functional immune system and the effectiveness of malaria vaccines require further
studies
In-vitro antibacterial activity of the seed extract of three-member Artocarpus towards Methicillin-Resistant Staphylococcus aureus (MRSA)
No full textMethicillin-Resistant Staphylococcus aureus (MRSA) infections have created a critical need for the development of natural antibacterials from a biological source. This research aimed to investigate the antibacterial activity of the seed extract of three-member Artocarpus (Artocarpus heterophyllus, A. champeden, and A. camansi) against MRSA which are the most prevalent causes of infections in patients. Crude seed extracts of three-member Artocarpus were evaluated for their antibacterial activity against MRSA. The antibacterial activity against MRSA of the three extracts was assayed in vitro by the agar well diffusion assay and agar microdilution method and minimum bactericidal concentration. The antibacterial activity, calculated as a zone of inhibition and MIC, MBC values. The Crude seed extracts of three-member Artocarpus showed antibacterial activity against the MRSA in the agar well diffusion assay (1.5-9 mm inhibition diameter). The MIC value of extract showed at 15.62 mg/mL and the MBC value of seed extract of A. heterophyllus at 62.5 mg/mL, A. champeden at 31.25 mg/mL, A. camansi at 250 mg/mL. All seed extracts have the potential to be developed as antibacterial agents, particularly against MRSA strain. Studies on the antibacterial activity against MRSA can provide new information about the benefits seed of members of Artocarpus as a source of natural antibacterial
