1,721,027 research outputs found
Isolation, screening and characterisation of cyanide-degrading Serratia marcescens strain aq07
Get PDFBiodegradation is now being considered as the best form of cyanide transformation because it is less expensive
and has fewer hazards than other conventional methods. Soil and drainage water samples from the premises of
Univerisiti Putra Malaysia (UPM) Serdang, were collected at different points and analysed for available bacteria. 28 bacteria were isolated from the samples and subjected to in-vitro cyanide remediation assay by using spectrophotometric method. Six bacterial isolates were observed to have cyanide-degrading ability. Screening was
conducted in three phases that include primary, secondary and tertiary screening. In the primary phase of the
screening, the six bacterial isolates were assayed for cyanide-degrading activity using 25 ppm filter-sterilised KCN in a buffer medium (pH 7.0) containing 5 g/L glucose, and 0.5% yeast extract. It was observed that three isolates have a greater potential of converting cyanide, degrading above 70%. The three isolates were further subjected to screening which is the secondary screening by incorporating 100 ppm of filter sterilised KCN. All three isolates showed good ability of degradation removing above 60% of the cyanide. The isolate tagged as AQ-007 was found to have a better degradation capability, removing 75% of the cyanide while isolate AQ-003 and AQ-004 degraded 61.5% and 64.6%
respectively. The tertiary screening phase was carried out using resting cells of the bacteria. The resting cells illustrates that it is the best form of inoculums. Isolates AQ-003 removed 92.8%, AQ-004 remediated 92.9% and AQ- 007 degraded 98% of the cyanide. Isolate AQ-007 was considered to be the preferred bacteria for further research because it degrades higher than the other two isolates. Bacterial identification based on 16s rRNA gene sequence analysis of this bacteria showed a 97% identity with Serratia marcescens. It has been registered in the GenBank as Serratia marcescens Strain AQ07 with accession number KP21329
Characterization and crystal structures of carbazole degrading genes (CarBaBb and CarC) from sphingomonas sp. KA1
Get PDFMolecular Cloning of a Functional Fads2 Promoter from Zebrafish
Get PDFThe FADS2 catalyzes the first rate-limiting step in the long chain-polyunsaturated fatty acids
(LC-PUFAs) biosynthesis pathway by converting �-linolenic acid and linoleic acid into
stearidonic acid and �-linolenic acid via the �-3 and �-6 pathways respectively. In mammals,
PPAR� and SREBP-1c have been implicated in the polyunsaturated fatty acids (PUFAs)
mediated transcriptional activation of FADS2 promoter. However, in zebrafish, not much is
known regarding the regulation of fads2 transcriptional regulation. Here, in this study, five
vectors containing different promoter regions were constructed in order to analyse putative
promoter activities. Through truncation analysis, it was found that the 1.2 kb promoter was able
to drive luciferase activity to an approximate 40-fold in HepG2 cells. Upon mutagenesis
analysis, three sites which are the putative NF-Y, SREBP and PPAR binding sites were found
to be essential in driving the promoter activity. Lastly, the 1.2 kb fads2 promoter was able to
direct EGFP expression specifically to the yolk syncytial layer (YSL) when transiently
expressed in microinjected zebrafish embryos
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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