98 research outputs found
Toimiva pienlehden ulkoasu : eväät pienlehden etätoimittamiseen ulkoasuvastaavalle
Get PDFTämä tutkielma käsittelee hyväksi havaittuja eväitä etänä toimivan pienlehden aloittelevalle ulkoasuvastaavalle. Käytän läpi tutkielmani esimerkkinä neljä kertaa vuodessa ilmestyvää turkulaista kirjallisuuslehti Lumoojaa, jonka kahdeksi vuodeksi kerrallaan valittavan toimituskunnan ulkoasuvastaavana toimin. Olen rajannut työni Lumoojan kaltaiseen kirjallisuuslehteen.
Aloitin Lumoojassa alkuvuodesta 2013, jolloin uudistin lehden ulkoasun täysin aiemmasta. Uudistus on saanut paljon positiivista palautetta lehden lukijoilta sekä toimituskunnalta. Talvella 2014 ilmestynyt toimituskuntamme viides Lumooja (1/14) toimii opinnäytetyöni toiminnallisena osana.
Käsittelen toimivia ulkoasuun liittyviä seikkoja omien Lumoojasta saamieni kokemusten ja havaintojen sekä alan lähdekirjallisuuden kautta. Käyn läpi aiheeseen liittyviä pien- ja kirjallisuuslehden sekä etätoimituksen käsitteitä ja luon tiiviin yleiskatsauksen tämänhetkiseen suomalaiseen pien- ja kirjallisuuslehtimaailmaan. Alun käsitteiden selvittämisestä etenen yleiseen lehden rakenteeseen. Avaan myös Lumoojaa pienlehtenä sekä sen toimituskunnan tehtäviä. Lisänä tutkielmani sisältää Lumoojan ulkoasu-uudistuksen prosessikuvauksen. Tutkielmani loppuosa sisältää yleisesti hyväksi havaittuja sekä havaitsemiani eväitä ja neuvoja, joiden avulla on mahdollista luoda visuaalisesti lukijan tarpeita parhaalla tavalla palveleva pienlehti. Tutkielmani soveltuu erityisen hyvin muun muassa graafisen suunnittelun opiskelijalle, joka vailla käytännön kokemusta on aloittamassa pienlehden ulkoasuvastaavana.This thesis contains verified tips for the layout designer who starts working in a remotely operating small magazine. The literary magazine Lumooja is the continuous example through the thesis. The author is responsible for the graphic design of this small magazine that has a two-year term elected editorial board. The study is limited to literary magazines
like Lumooja.
When the editorial board started their term in early 2013 the whole magazine layout was renewed by the author. The reform has received a lot of positive feedback. In winter 2014 the fifth Lumooja (1/14) was published which is the functional part of this thesis.
The thesis deals with the appearance of the functional aspects of Lumooja using the author’s experience and observations in the field, as well as literary sources. This study covers concepts related to small magazines and literary magazines, as well as distance edition concepts and it creates a concise overview of the current Finnish small and literary magazine field. The thesis includes original concepts of how to determine the general structure of the newspaper. This thesis also opens Lumooja as a small magazine and explains its editorial board's duties. Furthermore the thesis contains the description of Lumooja’s trench layout reform process. The last part of the thesis includes generally verified as well as the author’s tips and advice with the help of which it is possible to create a small magazine that visually serves readers' needs with the best possible way. This thesis is particularly valuable for a graphic design student that has no earlier practical experience of working in a small magazine.Opinnäytetyön toiminnallinen osuus löytyy Metropolian kirjaston Lummetien toimipisteestä Vantaan Tikkurilasta
Micro- and Nanoengineering Approaches to Developing Gradient Biomaterials Suitable for Interface Tissue Engineering
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Design and analysis of a single cell RNA-seq benchmarking dataset to compare protocols and methods.
No full textAuthors: Matthew Ritchie [1], Luyi Tian [1], Xueyi Dong [1], Saskia Freytag [1], Shian Su [1], Daniela Amann-Zalcenstein [1], Tom Weber [1], Azadeh Seidi [2], Kim-Anh Lê Cao [3], Shalin Naik [1]
Affiliations: 1. Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
2. Australian Genome Research Facility, Parkville, Australia.
3. Melbourne Integrative Genomics, The University of Melbourne, Parkville, Australia.
Abstract: Single cell RNA sequencing (scRNA-seq) technology has undergone rapid development in recent years and has brought with it new challenges in data processing and analysis. This has led to an explosion of tailored analysis methods for scRNA-seq to address various biological questions, from cell type identification, to marker gene discovery and trajectory analysis. The current lack of gold-standard benchmarking datasets makes it difficult for researchers to evaluate the performance of the many different methods available in a systematic manner.
To address this problem, we designed and generated a cross-platform benchmark dataset that has in-built truth in various forms as well as varying levels of biological noise. We use this dataset to compare different protocols, examine popular assumptions made in scRNA-seq analyses and compare methods for tasks ranging from normalization to trajectory analysis. We found significant differences in the results from the methods compared and have identified a few that performed well across protocols in high and low variability scenarios. Our dataset and analysis provide a valuable resource for understanding the nature of scRNA-seq data and can be used to guide algorithm selection in different biological settings.Non UBCUnreviewedAuthor affiliation: The Walter and Eliza Hall Institute of Medical ResearchOthe
A microfluidic-based neurotoxin concentration gradient for the generation of an<i>in vitro</i>model of Parkinson’s disease
No full textNovel Characteristics of Photodamage to PSII in a High-Light-Sensitive Symbiodinium Phylotype
No full textDinoflagellates from the genus Symbiodinium form symbiotic relationships with many marine invertebrates, including reef-building corals. Symbiodinium is genetically diverse, and acquiring suitable Symbiodinium phylotypes is crucial for the host to survive in habitat environments, such as high-light conditions. The sensitivity of Symbiodinium to high light differs among Symbiodinium phylotypes, but the mechanism that controls light sensitivity has not yet been fully resolved. In the present study using high-light-tolerant and -sensitive Symbiodinium phylotypes, we examined what determines sensitivity to high light. In growth experiments under different light intensities, Symbiodinium CS-164 (clade B1) and CCMP2459 (clade B2) were identified as high-light-tolerant and -sensitive phylotypes, respectively. Measurements of the maximum quantum yield of photosystem II (PSII) and the maximum photosynthetic oxygen production rate after high-light exposure demonstrated that CCMP2459 is more sensitive to photoinhibition of PSII than CS-164, and tends to lose maximum photosynthetic activity faster. Measurement of photodamage to PSII under light of different wavelength ranges demonstrated that PSII in both Symbiodinium phylotypes was significantly more sensitive to photodamage under shorter wavelength regions of light spectra (<470 nm). Importantly, PSII in CCMP2459, but not CS-164, was also sensitive to photodamage under the regions of light spectra around 470-550 and 630-710 nm, where photosynthetic antenna proteins of Symbiodinium have light absorption peaks. This finding indicates that the high-light-sensitive CCMP2459 has an extra component of photodamage to PSII, resulting in higher sensitivity to high light. Our results demonstrate that sensitivity of PSII to photodamage differs among Symbiodinium phylotypes and this determines their sensitivity to high light
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