1,720,979 research outputs found
Study of L-DOPA decarboxylase and kallikreins gene expression regulation through microRNA molecules, in prostate and bladder cancers: evaluation of their differential diagnostic and prognostic value
No full textOne of the main features of prostate and bladder cancers is their clinical heterogeneity, in terms of disease progression rate, patients’ survival and treatment response. Therefore, the studying of the different molecular signatures of the disease and the identification of novel molecular biomarkers could offer, an alternative approach to improve disease prognosis and to support personalized treatment decisions, and is of first research and clinical priority.L-DOPA decarboxylase (DDC) was recently identified as a novel co-activator of androgen receptor (AR), enhancing the ligand-dependent AR transcriptional activity. Additionally, DDC represents also a marker of the neuroendocrine (NE) differentiation of the prostate. The NE prostate cells play essential role in prostate cancer progression. Tissue kallikrein (KLK1) and the kallikrein-related peptidases (KLK2-KLK15) constitute a family of 15 homologous secreted serine proteases. The value of KLKs as cancer biomarkers is highlighted by the previously established clinical utility of prostate specific antigen (PSA), as kallikrein-related peptidase 3 (KLK3) is largely known, for prostate cancer diagnosis and treatment monitoring. Finally, microRNAs (miRNAs) are a rapidly growing family of small (~22nt) non-coding RNAs, representing the most powerful gene expression regulators at the post-transcriptional level. Their function is crucial for cellular homeostasis, and the deregulation of miRNAs expression has become a hallmark of the majority of human malignancies.In the present Ph.D. Thesis is performed the expression analysis and the evaluation of the clinical significance of DDC, KLKs genes, and miRNAs molecules, which regulate their expression post-transcriptionally, in prostate and bladder cancers. To fulfill these objectives, a statistically significant number of 137 prostate and 279 blabber fresh frozen tissues were collected, processed and included in the study. Moreover, novel molecular methodologies were developed and optimized for the accurate and sensitive quantification of the tested genes and miRNAs in prostate and bladder tissue specimens. Additionally, a detailed database of patients’ history, clincopathological and follow-up data has been constructed for all patients. The analysis of the total number of tissue specimens and the extended statistical analysis of the results of the study highlighted the significant clinical value of the tested biomarkers for the differential diagnosis and the accurate prognosis of prostate and bladder cancers.Ένα ιδιαίτερο χαρακτηριστικό του καρκίνου του προστάτη και του καρκίνου της ουροδόχου κύστεως είναι η έντονη κλινική ετερογένειά τους, σχετικά με την πρόγνωση εξέλιξης της νόσου, την επιβίωση των ασθενών και την ανταπόκρισή τους στην θεραπεία. Για το λόγο αυτό, η μελέτη του μοριακού προφίλ των όγκων και η ταυτοποίηση νέων μοριακών δεικτών μπορεί να προσφέρει σημαντικά στην βελτίωση της πρόγνωσης και τον σχεδιασμό της εξατομικευμένης θεραπείας των ασθενών, εμφανίζοντας έντονο ερευνητικό και κλινικό ενδιαφέρον σήμερα.Η L-DOPA αποκαρβοξυλάση (DDC) έχει βρεθεί να αποτελεί ένα νεότερο συνενεργοποιητή του υποδοχέα των ανδρογόνων (AR) ενισχύοντας σημαντικά την μεταγραφική ενεργότητά του. Επίσης, η DDC αποτελεί δείκτη της νευροενδοκρινούς (NE) διαφοροποίησης του προστάτη, με τα ΝΕ κύτταρα του αδένα να διαδραματίζουν κεντρικό ρόλο για την εξέλιξη της νόσου. Οι καλλικρεΐνες (KLKs) αποτελούν μια οικογένεια 15 ομόλογων εκκρινόμενων πρωτεασών σερίνης, η ιδιαίτερη κλινική αξία της οποίας στις ανθρώπινες νεοπλασίες αντικατοπτρίζεται στον προσδιορισμό του PSA, όπως είναι ευρέως γνωστή η KLK3, του ορού του αίματος για την διάγνωση και την πρόγνωση του καρκίνου του προστάτη. Τέλος, τα microRNAs (miRNAs) αποτελούν μια διαρκώς αυξανόμενη οικογένεια μικρών (~22 nt) μη-κωδικών μορίων RNA, τα οποία αποτελούν τους σημαντικότερους ρυθμιστές της γονιδιακής έκφρασης σε μετα-μεταγραφικό επίπεδο. Η δράση τους είναι ιδιαίτερα σημαντική για την κυτταρική ομοιόσταση, ενώ η απορρύθμιση της έκφρασής τους παρατηρείται στην πλειοψηφία των κακοηθειών του ανθρώπου.Στην παρούσα διδακτορική διατριβή πραγματοποιείται η μελέτη της έκφρασης και αξιολόγηση της κλινικής αξίας των DDC, KLKs γονιδίων, καθώς και miRNAs, τα οποία ρυθμίζουν μετα-μεταγραφικά την έκφρασή τους, στον καρκίνο του προστάτη και της ουροδόχου κύστεως. Για την επίτευξη των στόχων της διατριβής πραγματοποιήθηκε η συλλογή και επεξεργασία ενός στατιστικά σημαντικού αριθμού 137 ιστοτεμαχίων προστάτη και 279 ιστοτεμαχίων ουροδόχου κύστεως, καθώς και η ανάπτυξη και βελτιστοποίηση νέων μοριακών μεθοδολογιών προσδιορισμού των επιπέδων έκφρασης των υπό μελέτη γονιδίων και miRNAs. Λεπτομερής βάση των κλινικοπαθολογικών χαρακτηριστικών και της πορείας τους (follow-up) έπειτα από την θεραπεία κατασκευάστηκε για το σύνολο των ασθενών. Η βιοστατιστική ανάλυση των αποτελεσμάτων ανέδειξε την ισχυρή κλινική αξία, ενός σημαντικού αριθμού των υπό μελέτη βιομορίων, για την διαφορική διάγνωση και πρόγνωση του καρκίνου του προστάτη και της ουροδόχου κύστεως
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
- …
