1,721,044 research outputs found
Immunofluorescence and enzyme histochemistry on consecutive sections from glycol-methacrylate-embedded bone marrow, lymph node and kidney specimens.
No full textWe describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-micron-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)2 antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: (1) reduction of background fluorescence, (2) higher resolution of morphologic details, (3) possibility of studying undecalcified bone marrow specimens and (4) possibility of coupling histochemistry with immunofluorescence on consecutive sections. Moreover, in a case of lymphoplasmacytoid lymphoma specific immunostaining for membrane IgM was obtained on GMA sections
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Early onset of type 1 diabetes in a child with congenital cytomegalovirus (CMV) infection. A one year follow-up.
No full textAn eco-friendly enantioselective access to (R)-Naringenin as inhibitor of proinflammatory cytokine release.
No full text(R/S)-Naringenin is a flavanone, well-known for its beneficial health-related properties, such as anti-inflammatory activity. The preparative enantioselective chromatographic resolution of commercial (R/S)-Naringenin was performed on Chiralpak® AD-H column (500 x 50 mm I.D., dp = 20 μm) using methanol as eluent. The developed method is in accordance with the principles of green chemistry, since the environmental impact was lowered through the recycle of eluent. In this way, g-amounts of each enantiomer were recovered with high purity (chemical purity > 99%, enantiomeric excess > 94%). The racemic and enantiomeric Naringenin were subjected to an exhaustive in vitro investigation of anti-inflammatory activity, aimed at evaluating the relevance of chirality. The assay on cultured human peripheral blood mononuclear cells activated by Phytohemagglutinin A revealed that (R)-Naringenin is the most effective in inhibiting the T-cell proliferation, showed to be still active at lowest concentration. Moreover, (R)-Naringenin significantly reduced pro-inflammatory cytokines levels such as TNF-α and, with less potency, IL-6. These results evidenced the anti-inflammatory potential of Naringenin and the highest capacity of (R)-Naringenin to inhibit both in vitro PBMC proliferation and cytokines secretion at non toxic doses. Thus, (R)-Naringenin is a promising candidate for in vivo investigation
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Diabete mellito ad insorgenza precoce in un bimbo con infezione congenita da cytomegalovirus (CMV).
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