1,721,042 research outputs found
Cellular Immunity-Pathogen Interactions in Infectious Diseases
Get PDFAbubakar, Muhammad/0000-0002-0245-0254; ATMACA, HASAN TARIK/0000-0001-8379-4114
Central nervous system and skull malformations associated with Bovine Viral Diarrhea Virus in a calf
No full textOrhan, Ismail Onder/0000-0003-3006-4657; KUMANDAS, ALI/0000-0002-7679-2126; DINCEL, Gungor Cagdas/0000-0002-6985-3197; ATMACA, HASAN TARIK/0000-0001-8379-4114In this study, cranial anomalies, hydranencephaly and cerebellar hypoplasia were reported macroscopically, microscopically and immunohistochemically in a calf. A relationship between Bovine Viral Diarrhea Virus infection and malformations associated with central nervous system lesions were demonstrated
Clinicopathologic diagnosis of cutaneous toxoplasmosis in an Angora cat
No full textATMACA, HASAN TARIK/0000-0001-8379-4114; Suer, Cem/0000-0002-6455-6644This report deals with a case of cutaneous toxoplasmosis in a 2 year-old female Angora cat. Cutaneous lesions were characterized by prescapular ulcers and hyperemic nodules in the skin of the inguinal and dorsosacral regions. A skin biopsy sample was collected from the lesioned area and processed for histopathologic examination and immunoperoxidase test using Toxoplasma gondii and Neospora caninum specific antibodies. Toxoplasma gondii immunopositive reactions were detected in keratinocytes and dermal macrophages while no immunoreactivity was detected for N. caninum. The case of cutaneous toxoplasmosis was further confirmed by PCR analysis using T gondii B1 gene-specific primers. In conclusion, we report the first case of cutaneous toxoplasmosis in Angora cats
Role of oxidative stress in the pathophysiology of Toxoplasma gondii infection
No full textDINCEL, Gungor Cagdas/0000-0002-6985-3197; ATMACA, HASAN TARIK/0000-0001-8379-4114Oxidative stress (OS) plays an essential role in the pathogenesis of common neurodegenerative diseases. We have previously shown that Toxoplasma gondii (T. gondii) induces high nitric oxide (NO) production, glial activation, and apoptosis that altogether cause severe neuropathology in toxoplasma encephalitis (TE). The objective of this study was to investigate the cytotoxic effect of OS and to identify a correlation between the causes of T. gondii induced neuropathology. Expression levels of glutathione reductase (GR), Cu/Zn superoxide dismutase (SOD1), neuron specific enolase (NSE), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were investigated. Results of the study revealed that the levels of GR (P <0.005) and NSE (P<0.001) expression in the brain tissue markedly increased while SOD1 activity decreased (P<0.001) in the infected group compared to the non-infected group. In addition, intense staining for 8-OHdG (P<0.05) was observed both in the nucleus and the cytoplasm of neurons and glial cells that underwent OS. These results were reasonable to suggest that T. gondii-mediated OS might play a pivotal role and a different type of role in the mechanism of neurodegeneration/neuropathology in the process of TE. The results also clearly indicated that increased levels of NO and apoptosis might contribute to OS-related pathogenesis of TE. As a result, OS and expression of NSE might give an idea of the disease progress and may have a critical diagnostic significance for patients with T. gondii infection.Scientific Research Projects Commission of the Gumushane Univesity, Gumushane, Turkey [13.B0421.02.02]This work was funded and supported by the Scientific Research Projects Commission of the Gumushane Univesity, Gumushane, Turkey (Project Code: 13.B0421.02.02)
Protective effect of resveratrol on sodium fluoride-induced oxidative stress, hepatotoxicity and neurotoxicity in rats
No full textDas, Kusal/0000-0002-3641-2117; ATMACA, HASAN TARIK/0000-0001-8379-4114Protective effect of resveratrol on sodium fluoride-induced oxidative stress, hepatotoxicity and neurotoxicity were studied in rats. A total of 28 Wistar albino male rats were used. Four study groups were randomly formed with seven animals in each. The groups were treated for 21 days with distilled water (control group), with water containing 100 ppm fluoride (fluoride group), with resveratrol (12.5 mg/kg i.p., resveratrol group), or with 100 ppm fluoride + 12.5 mg/kg resveratrol i.p. (fluoride + resveratrol group). At the end of the trial, blood samples were collected by cardiac puncture and tissue samples were taken simultaneously. The total antioxidant and oxidant status in plasma and tissues as well as plasma 8-hydroxydeoxyguanosine levels were measured. Histopathological analyses of rat liver and brain tissues were performed in all groups to identify any changes. In the fluoride group, the total oxidant levels increased in plasma, liver and brain and total antioxidant levels decreased, as did the plasma 8-hydroxy-deoxyguanosine levels. These changes were prevented by co-administration of resveratrol. In addition, fluoride-associated severe histopathological changes in brain and liver tissues were not observed in the fluoride + resveratrol group. Consequently, these data suggested that resveratrol had beneficial effects in alleviating fluoride-induced oxidative stress. (C) 2014 Elsevier Ltd. All rights reserved.Kirikkale University Research FundKirikkale University [2011/44]Financial support for this Project (Project No: 2011/44) was provided by the Kirikkale University Research Fund
Left ventricular mass in a Simmental calf: cavernous haemangioma
No full textDINCEL, Gungor Cagdas/0000-0002-6985-3197; Terzi, Osman Safa/0000-0002-7877-8897; ATMACA, HASAN TARIK/0000-0001-8379-4114
Reactivated and clinical Toxoplasma gondii infection in young lambs: Clinical, serological and pathological evidences
No full textBabur, Cahit/0000-0002-6524-3260; ATMACA, HASAN TARIK/0000-0001-8379-4114In this study, fatal toxoplasmosis with severe neurological clinical signs was evaluated in 20 lambs of a sheep flock containing 90 Akkaraman sheep, 60 lambs and 3 rams. The clinical signs in infected lambs (n = 20) included incoordination, head shaking, tremors, shaking up, difficulty walking and subsequent death. No incidence of abortion observed in pregnant ewes. Two lambs with severe clinical signs were euthanized and necropsied following clinical and hematological examinations. Blood samples were also collected from the mothers of dead lambs and rams for Sabin-Feldman dye test to detect Toxoplasma gondii-specific antibodies. T. gondii infection was further confirmed by PCR analysis using T. gondii B1 gene specific primers. The histopathological findings included non-purulent myositis with T. gondii-like tissue cysts in the heart and skeletal musculature, severe necrotic vasculitis and multifocal necroses in the brain, liver and lungs. T. gondii immunoreactivity was present in the lungs, liver and spleen as well as tissue cyst-like structures. In differential diagnosis, Neospora caninum infection was excluded by immunohistochemical and PCR analyses. According to current literature, there has been no previous report on clinical toxoplasmosis in newborn lambs or goat kids, and the number of reports on clinical toxoplasmosis in small ruminants is limited to two adult goats with fatality following systemic toxoplasmosis. Our study indicated that toxoplasmosis occurs in lambs with severe neurological signs and subsequent death. (C) 2012 Elsevier B.V. All rights reserved.Kirikkale University Research CouncilKirikkale University [21/2006]This study is financially supported by Kirikkale University Research Council Project with 21/2006 project number. The clinical toxoplasmosis cases were presented as oral presentation at XXIVth World Buiatrics Congress, 2008 Hungary and at the 4th national Veterinary Pathology Congress, 2008, Antalya, Turkey
Evaluation of oxidative stress, hematological and biochemical parameters during Toxoplasma gondii infection in gerbils
No full textKABAKCI, Ruhi/0000-0001-9131-0933; ATMACA, HASAN TARIK/0000-0001-8379-4114The aim of the present study was to investigate the alterations of oxidative stress, hematological and biochemical parameters in experimental infection caused by Toxoplasma gondii in gerbil. A total of 16 gerbil, 8 of which were control and 8 was infection group, were used in the study. The gerbils were infected by intraperitoneal inoculation of 5000 T. gondii RH strain tachyzoites. In group of, the gerbil were sacrificed at 7th day after inoculation. At the end of this period, blood samples collected and erythrocyte malondialdehyde (MDA) concentrations, superoxide dismutase (SOD), catalase (CAT) activities, plasma aspartat aminotransferase (AST) and alanine aminotranspherase (ALT) activities, total protein, albumin, globulin were determined. Besides, hematological parameters were analysed in whole blood. Aspartat aminotransferase and ALT activities and MDA concentrations and neutrophil percentage and total leukocyte counts increased significantly in infected group when compared to control. In infected group, SOD activities, albumin concentrations and lymhocyte percentage decreased when compared to control. The results of this study suggest that oxidative stress, hematological and biochemical alterations may contribute to the pathogenesis of toxoplasmosis in gerbils
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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