13 research outputs found
<i>In-vitro</i> percental release of cortisol from superfused head kidneys of LR and HR sea bass individuals stimulated with ACTH.
No full textValues are expressed as percental secretion relative to basal and presented as means + SD (n = 6). The shaded areas represent the period over which the head kidneys were treated with 10−7 M ACTH.</p
Resting plasma cortisol and ACTH concentrations of LR and HR sea bass.
No full textCortisol (A) and ACTH (B) concentrations are presented as means + SD (n = 6). Means with different letters differ significantly from one another (t-test; P < 0.05).</p
Polyphasic Identification Of A New Thermotolerant Species Of Lactic Acid Bacteria Isolated From Chicken Faeces
No full textpeer reviewedTwo thermotolerant and desiccation tolerant lactic acid bacteria (TDLAB) were pointed out
from twenty isolated strains from soils and dried chicken faeces. Samples were collected in
poultry farms in the vicinity of Dakar, Senegal (West Africa). The two new isolates were called
Sp.4 (Sp.4=CWBI-B534=LMG7278) and Sp.20 (Sp.20=CWBI-B545=LMG7279). They are Grampositive, catalase-negative, facultatively anaerobic, non-motile, and non-spore-forming rods. Both produce D/L lactic acid via homofermentative pathway. Growth of the strains occurred between 15°C and 44°C. The optimum temperature for growth was in 30°C-37°C temperature and pH 3-8 range. Desiccation treatment in glycerol showed 30% survival rates. Complex total fatty acid pattern of the strains showed the presence of C14:0, C16:0, C16:1, C18:0, C18:1, C18:2, C18:3. SDS-PAGE of total protein of both strains placed them in L. plantarum group. AFLP analysis showed a phylogenetic proximity of the two strains with L. plantarum stricto sensu species. Specific amplified 16s rDNA restriction analysis (ARDRA) of the 16S rDNA gene, however, showed that these thermotolerant strains were not L. plantarum. ITS sequencing revealed that Sp.20 (LMG 7279) could be classed into Lactobacillus paraplantarum species since the short sequence of ITS showed 95% of similarity with reference species. Polyphasic identification shows that Sp.4, (the type strain is LMG 7278T) represent a new species within the genus Lactobacillus with only 88%+/-1 ITS sequence similarity with reference species. For which the name Lactobacillus aminata sp. nov. is proposed
Spearman rank correlation coefficient, <i>r</i>, between the concentration of plasma cortisol and ACTH, the relative gene expression of <i>mc2r</i>, <i>cyp11b1</i>, and <i>hsd11b2</i> and cortisol superfusion release rates (<i>n</i> = 6 for plasma; <i>n</i> = 5 for mRNA).
No full textSpearman rank correlation coefficient, r, between the concentration of plasma cortisol and ACTH, the relative gene expression of mc2r, cyp11b1, and hsd11b2 and cortisol superfusion release rates (n = 6 for plasma; n = 5 for mRNA).</p
Primer sequences used in qPCR for sea bass.
No full textPrimer sequences used in qPCR for sea bass.</p
Regulation of divergent cortisol responsiveness in European sea bass, <i>Dicentrarchus labrax</i> L. - Fig 2
No full textIn-vitro release of cortisol from superfused head kidneys of LR (A) and HR (B) sea bass stimulated with ACTH. Values are expressed as cortisol release rate (ng min-1 g-1) and presented as means + standard error of the mean (n = 6). The y-axis is in different scale. The shaded areas represent the period over which the head kidneys were treated with 10−7 M ACTH.</p
Regulation of divergent cortisol responsiveness in European sea bass, <i>Dicentrarchus labrax</i> L. - Fig 4
No full textRelative mRNA transcript levels of mc2r (A), cyp11b1 (B), and hsd11b2 (C) in the head kidneys of LR and HR sea bass. Values are presented as means + SD (n = 5). Means with different letters differ significantly from one another as shown by t-tests (t-tests; P < 0.05).</p
Regulation of divergent cortisol responsiveness in European sea bass, <i>Dicentrarchus labrax</i> L.
No full textMechanisms regulating differences in cortisol responsiveness between low (LR) and high response (HR) individuals have been poorly studied. In this context, we aimed to study key regulatory processes in cortisol dynamics at the head kidneys of LR and HR European sea bass. To do so, resting plasma cortisol and ACTH concentrations were quantified in these fish. Additionally, the head kidneys of these individuals were superfused through an in vitro superfusion system and stimulated with the same amount of ACTH to assess their cortisol biosynthetic capacity. Moreover, the expression of important genes in cortisol regulation was assessed. Results showed that LR fish had lower resting cortisol concentrations than HR, although no differences existed in the circulating levels of ACTH. Additionally, the biosynthetic capacity of HR was higher than that of LR fish when in vitro stimulated with ACTH. At the molecular level, a statistically significant 3.4-fold higher expression of the ACTH receptor, mc2r, and a 2.3-fold, though not significant, higher expression of 11β-hydroxylase (cyp11b1), an enzyme involved in cortisol biosynthesis, was observed in the HR fish. Finally, a statistically significant 1.3-fold lower expression of 11β-hydroxysteroid dehydrogenase 2 (hsd11b2), an enzyme involved in cortisol inactivation, was observed in HR when compared to LR fish. Therefore, it was for the first time indicated that cortisol dynamics can also be regulated at the post-production level in the head kidney. Collectively, our results highlight the crucial role of the interrenal tissue in the regulation of differences in cortisol response between LR and HR sea bass individuals.</div
Multiple Loci Are Associated with White Blood Cell Phenotypes
No full textWhite blood cell (WBC) count is a common clinical measure from complete blood count assays, and it varies widely among healthy individuals. Total WBC count and its constituent subtypes have been shown to be moderately heritable, with the heritability estimates varying across cell types. We studied 19,509 subjects from seven cohorts in a discovery analysis, and 11,823 subjects from ten cohorts for replication analyses, to determine genetic factors influencing variability within the normal hematological range for total WBC count and five WBC subtype measures. Cohort specific data was supplied by the CHARGE, HeamGen, and INGI consortia, as well as independent collaborative studies. We identified and replicated ten associations with total WBC count and five WBC subtypes at seven different genomic loci (total WBC count-6p21 in the HLA region, 17q21 near ORMDL3, and CSF3; neutrophil count-17q21; basophil count-3p21 near RPN1 and C3orf27; lymphocyte count-6p21, 19p13 at EPS15L1; monocyte count-2q31 at ITGA4, 3q21, 8q24 an intergenic region, 9q31 near EDG2), including three previously reported associations and seven novel associations. To investigate functional relationships among variants contributing to variability in the six WBC traits, we utilized gene expression-and pathways-based analyses. We implemented gene-clustering algorithms to evaluate functional connectivity among implicated loci and showed functional relationships across cell types. Gene expression data from whole blood was utilized to show that significant biological consequences can be extracted from our genome-wide analyses, with effect estimates for significant loci from the meta-analyses being highly corellated with the proximal gene expression. In addition, collaborative efforts between the groups contributing to this study and related studies conducted by the COGENT and RIKEN groups allowed for the examination of effect homogeneity for genome-wide significant associations across populations of diverse ancestral backgrounds
Supplemental Material, sj-docx-1-cpt-10.1177_10742484211069422 - Associations of Atrial Fibrillation Patterns With Mortality and Cardiovascular Events: Implications of the MISOAC-AF Trial
No full textSupplemental Material, sj-docx-1-cpt-10.1177_10742484211069422 for Associations of Atrial Fibrillation Patterns With Mortality and Cardiovascular Events: Implications of the MISOAC-AF Trial by Amalia Baroutidou, Anastasios Kartas, Athanasios Samaras, Andreas S. Papazoglou, Eleni Vrana, Dimitrios V. Moysidis, Evangelos Akrivos, Anastasios Papanastasiou, Ioannis Vouloagkas, Michail Botis, Evangelos Liampas, Artemios G. Karagiannidis, Efstratios Karagiannidis, Georgios Efthimiadis, Haralambos Karvounis, Apostolos Tzikas and George Giannakoulas in Journal of Cardiovascular Pharmacology and Therapeutics</p
