23 research outputs found
Root-reducing capacity, rhizosphere acidification, peroxidase and catalase activities and nutrient levels of Citrus taiwanica and C. volkameriana seedlings, under Fe deprivation conditions
No full text– The Fe deficiency created by either deprivation of Fe, low Fe supply or addition of CaCO3/NaHCO3 to Hoagland nutrient solution resulted in significant changes of the physiological and biochemical parameters of the rootstocks C. taiwanica and C. volkameriana. For instance, the lack of Fe and low Fe supply of 3 mM Fe-EDDHA induced an increase in the Fe root-reducing capacity and rhizosphere acidification. Seedlings treated with adequate Fe (20 mM Fe-EDDHA) showed a decline in the Fe-reducing capacity and in rhizosphere acidification. Fe deficiency was associated with a significant decline in peroxidase and catalase activities. Fe deprivation conditions increased the concentration of N, P, Ca, Mg and Mn and reduced the concentration of Fe and Zn in young leaves of both rootstocks. C. volkameriana was found to be more sensitive to Fe chlorosis than C. taiwanica.– Impact de la privation de Fe sur la capacité racinaire de réduction, l’acidification rhizosphérique, l’activité de peroxidase et catalase ainsi que sur le niveau nutritif chez le Citrus taiwanica et le C. volkameriana. Le déficit en fer provoqué par la privation, l’apport à faible dose ou l’addition de CaCO3/NaHCO3 provoque des modifications physiologiques et biochimiques chez les souches du C. taiwanica et du C. volkameriana. Par exemple, le déficit en fer induit une augmentation de la capacité racinaire de réduction du Fe, ainsi qu’une acidification de la rhizosphère. Des plants de semis présentent une diminution de la capacité de réduction du fer lorsqu’ils sont traités avec une quantité suffisante de Fe (20 mM MM Fe-EDDHA). Le déficit en fer est associé à l’activité de la catalase et de la peroxidase. Il est également observé que la privation de Fe augmente la concentration de N, P, Ca, Mg, Mn et diminue celle de Fe et de Zn chez les feuilles jeunes des deux souches. C. volkameriana est plus sensible à la chlorose en fer que C. taiwanica
Nonpharmacological interventions for managing respiratory symptoms in lung cancer
No full textPatients with lung cancer experience significant symptom burden, particularly symptoms of a respiratory nature. Such symptom burden can be distressing for patients and negatively impact their functional status and quality of life. The aim of this review is to evaluate studies of nonpharmacological and noninvasive interventions for the management of respiratory symptoms experienced by patients with lung cancer. In total, 13 studies met the inclusion criteria for this review and included 1383 participants of which 1296 were lung cancer patients. The most frequently assessed and reported symptom was breathlessness (n = 9 studies). Cough and haemoptysis were reported in one study. A variety of outcome measurement tools were used and a broad range of intervention strategies evaluated. Lack of consistency between studies impinged on the ability to combine studies. It is not possible to draw any firm conclusion as to the effectiveness of nonpharmacological interventions for the management of respiratory symptoms in lung cancer. Nonpharmacological interventions may well have an important role to play in the management of some of the respiratory symptoms (or combinations of respiratory symptoms), but more work of higher quality is necessary in the future. © The Author(s) 2012
Comparative Physiological and Proteomic Analysis Reveal Distinct Regulation of Peach Skin Quality Traits by Altitude
No full textThe role of environment in fruit physiology has been established; however, knowledge regarding the effect of altitude in fruit quality traits is still lacking. Here, skin tissue quality characters were analysed in peach fruit (cv. June Gold), harvested in 16 orchards located in low (71.5 m mean) or high (495. m mean) altitutes sites. Data indicated that soluble solids concentration and fruit firmness at commercial harvest stage were unaffected by alitute. Peach grown at high-altitude environment displayed higher levels of pigmentation and specific antioxidant-related activity in their skin at the commercial harvest stage. Skin extracts from distinct developmental stages and growing altitudes exhibited different antioxidant ability against DNA strand-scission. The effects of altitude on skin tissue were further studied using a proteomic approach. Protein expression analysis of the mature fruits depicted altered expression of 42 proteins that are mainly involved in the metabolic pathways of defense, primary metabolism, destination/storage and energy. The majority of these proteins were up-regulated at the low-altitude region. High-altitude environment increased the accumulation of several proteins, including chaperone ClpC, chaperone ClpB, pyruvate dehydrogenase E1, TCP domain class transcription factor and lipoxygenase. We also discuss the altitude-affected protein variations, taking into account their potential role in peach ripening process. This study provides the first characterization of the peach skin proteome and helps to improve our understanding of peach’s response to altitude
Corrections to “Mapping unmet supportive care needs, quality-of-life perceptions and current symptoms in cancer survivors across the Asia-Pacific region: results from the International STEP Study”
No full textIn the original article, there were some errors in the numbers and accompanying text in the Abstract. These have now been corrected to match the numbers in the rest of the Article. Author M.-S. Li's name has been corrected to M. Yi and affiliation 7 has also been corrected to add 'Mahidol University, Bangkok' after 'Faculty of Nursing'.No Full Tex
Genomics Opportunities and Breeding Strategies Towards Improvement of Climate-Smart Traits and Disease Resistance Against Pathogens in Sweet Cherry
No full textAuthor Correction: Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenol
No full textA correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.</jats:p
The impact of sodium nitroprusside and ozone in kiwifruit ripening physiology: A combined gene and protein expression profiling approach
No full textBackground and Aims Despite their importance in many aspects of plant physiology, information about the function of oxidative and, particularly, of nitrosative signalling in fruit biology is limited. This study examined the possible implications of O3 and sodium nitroprusside (SNP) in kiwifruit ripening, and their interacting effects. It also aimed to investigate changes in the kiwifruit proteome in response to SNP and O3 treatments, together with selected transcript analysis, as a way to enhance our understanding of the fruit ripening syndrome. Methods Kiwifruits following harvest were pre-treated with 100∈μm SNP, then cold-stored (0°C, relative humidity 95%) for either 2 or 6 months in the absence or in the presence of O3 (0·3∈μL L-1), and subsequently were allowed to ripen at 20°C. The ripening behaviour of fruit was characterized using several approaches: together with ethylene production, several genes, enzymes and metabolites involved in ethylene biosynthesis were analysed. Kiwifruit proteins were identified using 2-D electrophoresis coupled with nanoliquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Expression patterns of kiwifruit ripening-related genes were also analysed using real-time quantitative reverse transcription-PCR (RT-qPCR). Key Results O3 treatment markedly delayed fruit softening and depressed the ethylene biosynthetic mechanism. Although SNP alone was relatively ineffective in regulating ripening, SNP treatment prior to O3 exposure attenuated the O3-induced ripening inhibition. Proteomic analysis revealed a considerable overlap between proteins affected by both SNP and O3. Consistent with this, the temporal dynamics in the expression of selected kiwifruit ripening-related genes were noticeably different between individual O3 and combined SNP and O3 treatments. Conclusions This study demonstrates that O3-induced ripening inhibition could be reversed by SNP and provides insights into the interaction between oxidative and nitrosative signalling in climacteric fruit ripening. © 2015 The Author 2015. Published by Oxford University Press on behalf of the Annals of Botany Company
Ozone-induced inhibition of kiwifruit ripening is amplified by 1-methylcyclopropene and reversed by exogenous ethylene
No full textBackground: Understanding the mechanisms involved in climacteric fruit ripening is key to improve fruit harvest quality and postharvest performance. Kiwifruit (Actinidia deliciosa cv. 'Hayward') ripening involves a series of metabolic changes regulated by ethylene. Although 1-methylcyclopropene (1-MCP, inhibitor of ethylene action) or ozone (O3) exposure suppresses ethylene-related kiwifruit ripening, how these molecules interact during ripening is unknown. Results: Harvested 'Hayward' kiwifruits were treated with 1-MCP and exposed to ethylene-free cold storage (0 °C, RH 95%) with ambient atmosphere (control) or atmosphere enriched with O3 (0.3 μL L- 1) for up to 6 months. Their subsequent ripening performance at 20 °C (90% RH) was characterized. Treatment with either 1-MCP or O3 inhibited endogenous ethylene biosynthesis and delayed fruit ripening at 20 °C. 1-MCP and O3 in combination severely inhibited kiwifruit ripening, significantly extending fruit storage potential. To characterize ethylene sensitivity of kiwifruit following 1-MCP and O3 treatments, fruit were exposed to exogenous ethylene (100 μL L- 1, 24 h) upon transfer to 20 °C following 4 and 6 months of cold storage. Exogenous ethylene treatment restored ethylene biosynthesis in fruit previously exposed in an O3-enriched atmosphere. Comparative proteomics analysis showed separate kiwifruit ripening responses, unraveled common 1-MCP- and O3-dependent metabolic pathways and identified specific proteins associated with these different ripening behaviors. Protein components that were differentially expressed following exogenous ethylene exposure after 1-MCP or O3 treatment were identified and their protein-protein interaction networks were determined. The expression of several kiwifruit ripening related genes, such as 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1), ethylene receptor (ETR1), lipoxygenase (LOX1), geranylgeranyl diphosphate synthase (GGP1), and expansin (EXP2), was strongly affected by O3, 1-MCP, their combination, and exogenously applied ethylene. Conclusions: Our findings suggest that the combination of 1-MCP and O3 functions as a robust repressive modulator of kiwifruit ripening and provide new insight into the metabolic events underlying ethylene-induced and ethylene-independent ripening outcomes. © 2018 The Author(s)
Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenol
No full textOrtho-phenylphenol (OPP) is a fungicide contained in agro-industrial effluents produced by fruit-packaging plants. Within the frame of developing bio-strategies to detoxify these effluents, an OPP-degrading Sphingomonas haloaromaticamans strain was isolated. Proteins/genes with a putative catabolic role and bacterium adaptation mechanisms during OPP degradation were identified via genomic and proteomic analysis. Transcription analysis of all putative catabolic genes established their role in the metabolism of OPP. The formation of key transformation products was verified by chromatographic analysis. Genomic analysis identified two orthologous operons encoding the ortho-cleavage of benzoic acid (BA) (ben/cat). The second ben/cat operon was located in a 92-kb scaffold along with (i) an operon (opp) comprising genes for the transformation of OPP to BA and 2-hydroxypenta-2,4-dienoate (and genes for its transformation) and (ii) an incomplete biphenyl catabolic operon (bph). Proteomics identified 13 up-regulated catabolic proteins when S. haloaromaticamans was growing on OPP and/or BA. Transcription analysis verified the key role of the catabolic operons located in the 92-kb scaffold, and flanked by transposases, on the transformation of OPP by S. haloaromaticamans. A flavin-dependent monoxygenase (OppA1), one of the most up-regulated proteins in the OPP-growing cells, was isolated via heterologous expression and its catabolic activity was verified in vitro. © 2017 The Author(s)
