5 research outputs found
ANTI-COLON CANCER EFFECT OF ORIGANUM MAJORANA ESSENTIAL OIL
No full textPlants have been shown to be an excellent source of new drugs, including anticancer agents. Origanum majorana commonly known as marjoram is a plant that is known to possess different therapeutic values including antioxidant andantimicrobial activities.
Our research team has previously tested the ethanolic extract of O. majorana on triple negative breast cancer and published the findings. The ethanolic extract promoted mitotic arrest at G2/M phase, induced apoptosis as well as inhibition of migration and metastasis. The promising potential of the ethanolic extract encouraged us to test the effects of O. majorana essential oil on human colon cancer cell lines.
We demonstrated that O. majorana essential oil inhibited the proliferation of HT- 29 and Caco-2 colon cancer cell lines in a time- and dose- dependent manner. Colony forming assay illustrated that O. majorana essential oil reduced the ability of HT-29 to form colonies, and when established colonies were treated with the essential oil, it showed that the treatment was able to reduce colonies’ size at lowconcentrations while at higher concentrations, the oil was able to completely eliminate the already formed colonies. Moreover, the essential oil, induced cell death and minimal cell cycle arrest at G1 phase. Annexin V staining revealed induction of apoptosis in HT-29 cells treated with the essential oil. Western blot assessment further confirmed apoptosis for being the main programmed cell death mechanism triggered by the plant’s essential oil. Blotting for survivin, which is a protein that belongs to the inhibitor of apoptosis protein (IAP) family, levels indicate that O. majorana essential oil exerts its cytotoxic anti-cancer effect at least partially through the down-regulation of survivin. These preliminary results make O. majorana oil a promising alternative candidate against colon cancer
Origanum majorana ethanolic extract promotes colorectal cancer cell death by triggering abortive autophagy and activation of the extrinsic apoptotic pathway
No full textColorectal cancer is considered as the third leading cause of cancer death. In the present study, we investigated the potential anticancer effect and the molecular mechanism of Origanum majorana ethanolic extract (OME) against human colorectal cancer cells. We showed that OME exhibited strong anti-proliferative activity in a concentration-and time-dependent manner against two human colorectal cancer cell lines (HT-29 and Caco-2). OME inhibited cell viability, colony growth and induced mitotic arrest of HT-29 cells. Also, OME induced DNA damage, triggered abortive autophagy and activated a caspase 3 and 7-dependent extrinsic apoptotic pathway, most likely through activation of the TNFα pathway. Time-course analysis revealed that DNA damage occurred concomitantly with abortive autophagy after 4 h post-OME treatment while apoptosis was activated only 24 h later. Blockade of autophagy initiation, by 3-methyladenine, partially rescued OME-induced cell death. Cell viability arose from 37% in control group to 67% in group pre-treated with 3-MA before addition of OME. Inhibition of apoptosis, however, had a minimal effect on cell viability; it rose from 37% in control group to 43% in group pre-treated with Z-VAD-FMK. We also found that OME downregulated survivin in HT-29 cells. Our findings provide a strong evidence that O. majorana extract possesses strong anti-colon cancer potential, at least, through induction of autophagy and apoptosis. These finding provide the basis for therapeutic potential of O. majorana in the treatment of colon cancer. © 2019 Benhalilou, Alsamri, Alneyadi, Athamneh, Alrashedi, Altamimi, Al Dhaheri, Eid and Iratni
Update on Vitamin B12 Deficiency
No full textAbstract: Vitamin B12 (cobalamin) deficiency is a common cause of megaloblastic anemia, a variety of neuropsychiatric symptoms and elevated serum homocysteine levels, particularly in the elderly. There are a number of risk factors for vitamin B12 deficiency, including long-term use of metformin and proton pump inhibitors. No major medical organization, including the U. Preventive Services Task Force, has published guidance on screening for vitamin B12 deficiency in asymptomatic or low-risk adults, but high-risk patients, such as B. those with malabsorption disorders, can order a detection.
The initial laboratory evaluation of a patient suspected of having vitamin B12 deficiency should include a complete blood count and serum vitamin B12 level. can be used to confirm deficiency in asymptomatic, high-risk patients with low normal vitamin B12 levels. Because crystalline formulations are better absorbed than natural vitamin B12, patients over the age of 50 and strict vegetarians should consume vitamin B12-fortified foods and vitamin B12 supplements rather than attempting to obtain vitamin B12 from dietary sources only
Administering vitamin B12 to patients with elevated serum homocysteine levels has not been shown to decrease cardiovascular outcomes in high-risk patients or alter cognitive decline in patients with mild to moderate Alzheimer's disease.
Keywords: vitamin B12 deficiency, medical organization, decrease cardiovascular, moderate Alzheimer's disease.
Title: Update on Vitamin B12 Deficiency
Author: Mohammed Saleh AlQahtani, Sami saleh almalki, MOHAMMED ALI AL MUJRI, FAISAL FAHAD ALMOTAIRI, Mohammed shatwi alqahtani,Thabit Ehsan Arnous, Mohammed Shaye alqhtani, Bader Ali Hazazi, Kholoud Rabah Alrashedi, Ghadeer Mohd alonazi
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Vol. 10, Issue 4, October 2022 - December 2022
Page No: 97-103
Research Publish Journals
Website: www.researchpublish.com
Published Date: 29-December-2022
DOI: https://doi.org/10.5281/zenodo.7492164
Paper Download Link (Source)
https://www.researchpublish.com/papers/update-on-vitamin-b12-deficiencyInternational Journal of Life Sciences Research, ISSN 2348-313X (Print), ISSN 2348-3148 (online), Research Publish Journals, Website: www.researchpublish.co
ATTITUDES AND BEHAVIOUR REGARDING DEEP DENTIN CARIES REMOVAL AMONG DENTAL HAIL STUDENT
No full textThe main objective of the study was to assess the attitude and behavior of the dental students in Hail region about the deep dental caries removal. The study was conducted through an online survey among the different dental students. A questionnaire was prepared online and was distributed to about hundred dental students out of which only 50 students responded to the survey. With the help of the questionnaire we were able to assess the students’ knowledge and attitude towards deep dental caries removal. Majority of the students selected hardness as a criteria for assessing the excavation. So most of them preferred the stepwise carries excavation. Higher percentage of the dental students were familiar with the deep dental caries removal treatments. The growing interest among Different researchers about the evaluation of knowledge about the deep dentin caries removal indicates the importance of this subject. Thus more comprehensive study is need to be carried out on different aspects of the deep dentin caries removal.
Keywords: Deep dentin caries removal, excavation, stepwise carries excavation
Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells
No full textColorectal cancer (CRC) is the third most common type of cancer in terms of incidence and mortality worldwide. Here we have investigated the anti-colon cancer potential of Origanum majorana essential oil (OMEO) and its underlying mechanisms of action. We showed that OMEO significantly inhibited the cellular viability and colony growth of human HT-29 colorectal cancer cells. OMEO induced protective autophagy, associated with downregulation of the mTOR/p70S6K pathway, and activated caspase-8 and caspase-9-dependent apoptosis. Blockade of autophagy with 3-methyladenine (3-MA) and chloroquine (CQ), two autophagy inhibitors, potentiated the OMEO-induced apoptotic cell death. Inversely, inhibition of apoptosis with the pan-caspase inhibitor, Z-VAD-FMK, significantly reduced cell death, suggesting that apoptosis represents the main mechanism of OMEO-induced cell death. Mechanistically, we found that OMEO induces protective autophagy and apoptotic cells death via the activation of the p38 MAPK signaling pathway. Pharmacological inhibition of p38 MAPK by the p38 inhibitors SB 202190 and SB 203580 not only significantly decreased apoptotic cell death, but also reduced the autophagy level in OMEO treated HT-29 cells. Strikingly, we found that OMEO also induces p38 MAPK-mediated caspase-dependent cleavage of p70S6K, a protein reported to be overexpressed in colon cancer and associated with drug resistance. Our findings suggest that OMEO inhibits colon cancer through p38 MAPK-mediated protective autophagy and apoptosis associated with caspase-dependent cleavage of p70S6K. To the best of our knowledge, this study is the first to report on the implications of the p38 MAPK signaling pathway in targeting p70S6K to caspase cleavage
