1,721,134 research outputs found
Milk thistle impedes the development of carbontetrachloride-induced liver damage in rats through suppression of bcl-2 and regulating caspase pathway
No full textWOS: 000345196900003PubMed: 25305509Aim: The objective of this study was to examine whether MT plays a protective role against the damage in the liver by administering carbontetrachloride (CCl4) to rats. Main method: 28 male Wistar albino (n = 28, 8 weeks old) rats have been used in the study. The rats were distributed into 4 groups according to their live weights. The groups were: (i) negative control (NC): normal water consuming group to which no CCl4 and milk thistle (MT) is administered; (ii) positive control (PC): normal water consuming group to which no Cat is administered but MT is administered; (iii) CCl4 group: normal water consuming and group to which CCl4 is administered (2 ml/kg live weight, ip); and (iv) CCl4 + MT group: CCl4 and MT administered group (2 ml/kg live weight. ip). Caspase-3, caspase-9, bax, and bcl-2 protein syntheses were examined via western blotting. MDA determination in liver tissue was made using spectrophotometer. Key findings: MDA amount has decreased in the CCl4 + MT group in comparison to CCl4 group whereas caspase-3 and caspase-9 has increased and bax and bcl-2 has decreased. Significance: These results show that MT protects the liver against oxidative damage.Firat University Scientific Research Projects Unit (FUBAP) [FF.13.19]The article supported by Firat University Scientific Research Projects Unit (FUBAP) with FF.13.19 project number. Some results of this article were presented at the 22nd National Biology Meeting (2014), Eskisehir, Turkey
Protein expression product alterations in Saccharomyces cerevisiae
No full textWOS: 000399099500009Lemon is very rich in terms of vitamin C and thus it has antioxidant properties. In this study, the effects of lemon juice on cellular development in yeasts and protein expression have been examined. Seven groups were created in this study: 1: Control group; 2: K2Cr2O7 group; 3: 5 mM K2Cr2O7 + lemon juice (LJ) group; 4: 10 mM K2Cr2O7 + LJ group; 5: 15 mM K2Cr2O7 + LJ group; 6: 20 mM K2Cr2O7 + LJ group; 7: 25 mM K2Cr2O7 + LJ group. After sterilization, fruit juice (15%) and K2Cr2O7 were added different concentration to Saccharomyces cerevisiae (S. cerevisiae) cultures and the cultures were developed at 30 degrees C for 1h, 3h, 5h and 24 hours (overnight). S. cerevisiae cell growth was analysed by spectrophotometer, total protein alterations was identified by SDS-PAGE electrophoresis and measured with biuret method. As a results: cell growth and protein expression amount increased in LJ groups to which LJ was taken in comparison to the positive control (K2Cr2O7) group at different growing times (1, 3, 5 and 24 hours) (p<0,05). These results indicated LJ has a protective effect for reduce the oxidative damage and raised cell growing and encourage protein synthesis in S. cerevisiae culture
The effect of orange juice against to H2O2 stress in Saccharomyces cerevisiae
Get PDFAslan, Abdullah (Aksaray, Yazar)In this study, seven groups were composed. i: Control group, ii: H2O2 group, iii: 5 mM H2O2 + orange juice (OJ) group, iv: 10 mM H2O2 + OJ group, v: 15 mM H2O2 + OJ group vi: 20 mM H2O2 + OJ group, vii: 25 mM H2O2 + OJ group. After sterilization, fruit juice (25%) and H2O2 were inserted different concentration to Saccharomyces cerevisiae (S. cerevisiae) cultures and the cultures were developed at 37C for 1h, 3h, 5h and 24 hours (overnight). S. cerevisiae cell growth was determined by spectrophotometer, total protein alteration was identified by SDS-PAGE electrophoresis and calculated with biuret method. With respect to our studies results; cell growth rised in fruit juice groups to which OJ was taken in proportion to the positive control (H2O2) group at different growing times (1, 3, 5 and 24 hours) (p0,05). As a result orange fruit juices has a protective role for decrease the oxidative damage and increased cell growing and stimulating protein synthesis in S. cerevisiae. © Mattioli 1885
The inhbition of chromium effect in Saccharomyces cerevisiae thrive from grapefruit
No full textAslan, Abdullah (Aksaray, Yazar)In this study, seven groups were composed. i: Control group, ii: K2Cr2O7 group, iii: 5 mM K2Cr2O7+ grapefruit juice (GFJ) group, iv: 10 mM K2Cr2O7 + GFJ group, v: 15 mM K2Cr2O7 + GFJ group, vi: 20 mM K2Cr2O7 + GFJ group, vii: 25 mM K2Cr2O7 + GFJ group. After sterilization, fruit juice (25%) and K2Cr2O7 were inserted different concentration to Saccharomyces cerevisiae (S. cerevisiae) cultures and the cultures were developed at 37°C for 1h, 3h, 5h and 24 hours (overnight). S. cerevisiae cell growth was determined by spectrophotometer, total protein changes was detected by SDS-PAGE electrophoresis and reckoned with biuret method. According to our studies results; cell growth rised in GFJ groups to which GFJ was taken in comparison to the positive control (K2Cr2O7) group at different growing times (1, 3, 5 and 24 hours) (p<0,05). As a result GFJ has a protecting for decrease the oxidative damage and increased cell growing and induced protein synthesis in S. cerevisiae culture
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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