1,721,075 research outputs found

    Flavobacterium granuli sp nov., isolated from granules used in a wastewater treatment plant

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    A Gram-negative, rod-shaped, non-spore-forming bacterium (designated strain Kw05(T)) was isolated from granules used in the wastewater treatment plant of a beer-brewing factory in Kwang-Ju, Republic of Korea. On the basis of 16S rRNA gene sequence similarity, strain Kw05(T) was shown to belong to the family Flavobacteriaceae, and was most closely related to Flavobacterium limicola (96-6%), Flavobacterium hibernum (96-3%), Flavobacterium hydatis (96-1%) and Flavobacterium xinjiangense (96-1%). The G+C content of the genomic DNA of strain Kw05(T) was 36-2 mol%, within the range of 32-37 mol% for the genus Flavobacterium. Chemotaxonomic data (major menaquinone MK-6; major fatty acids iso-C-15: 0, isoC(17: 0) 3-OH, iso-C-15:0 3-OH and iso-C-17:1 omega 9C) supported the classification of strain Kw05(T) within the genus Flavobacterium. Kw05(T) therefore represents a novel species, for which the name Flavobacterium granuli sp. nov. is proposed. The type strain is Kw05(T) ( = KCTC 12201(T)= IAM 15099(T))

    Phenylobacterium koreense sp nov., isolated from South Korea

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    T A Gram-negative, aerobic, rod-shaped, non-spore-forming bacterium, Slu-01(T) , was isolated from activated sludge from a wastewater treatment plant in Daejeon, South Korea. After 3 days on R2A medium, strain Slu-01(T) developed colourless colonies (0(.)-7-1(.)2 mm). The non-motile rods (0(.)7-1(.)0 x 1(.)0-2(.)0 mu m) were slightly curved and occurred singly and in pairs. No filamentous cells were found. On the basis of 16S rRNA gene sequence similarity, strain SlU-01(T) was shown to belong to the family Caulobacteraceae and was most closely related to Phenylobacterium immobile (96(.)6 %) and Phenylobacterium lituiforme (96(.)5 %). The genomic DNA G + C content of strain SlU-01(T) was 68(.)1 mol%, within the range of 67(.)3-68(.)4 mol% for the genus Phenylobacterium. Chemotaxonomic data (major ubiquinone, Q-10; major fatty acids C(18:1)omega 7c, C-16:0, C-15:0 and C-17:0) and 16S rRNA gene sequence analysis supported the affiliation of strain SlU-01(T) to the genus Phenylobacterium. However, DNA-DNA hybridization data and phenotypic properties showed that strain Slu-01(T) could be distinguished from the two other Phenylobacterium species with validly published names. Thus, it is suggested that strain Slu-01(T) represents a novel species of the genus Phenylobacterium, for which the name Phenylobacterium koreense is proposed. The type strain is SlU-01(T) (=KCTC 12206(T) = IAM 15119(T))

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Methylobacterium jeotgali sp nov., a nonpigmented, facultatively methylotrophic bacterium isolated from jeotgal, a traditional Korean fermented seafood

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    A novel facultatively methylotrophic, Gram-negative, rod-shaped bacterium, designated strain S2R03-9(T), was isolated from jeotgal, a traditional Korean fermented seafood. The organism was strictly aerobic, motile by means of a single polar flagellum, non-sporulating and catalase- and oxidase-positive. Strain S2RO3-9(T) grew in the presence of 0-1 % (w/v) NaCl and at pH 6.0-10.0, with optimum growth in the absence of NaCl and at pH 7.0. It grew at temperatures in the range 20.0-30.0 degrees C, with optimum growth at 30 degrees C. Colonies grown on R2A medium were nonpigmented, opaque and creamy white. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it was most closely related to Methylobacterium organophilum JCM 2833(T) (96.6% similarity) and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 95.0%. The DNA G + C content was 64.9 mol%. The phylogenetic analysis, the phenotypic assessment and the chemotaxonomic data (major ubiquinone, Q-10; major fatty acids, C-18:1 and C-18:0) showed that S2RO3-9(T) represents a novel species within the genus Methylobacterium in the class Alphaproteobacteria, for which the name Methylobacterium jeotgali sp. nov. is proposed. The type strain is S2RO3-9(T) (= KCTC 12671(T) = LMG 23639(T))

    Starkeya koreensis sp nov., isolated from rice straw

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    A facultatively chemolithoautotrophic, Gram-negative, aerobic, highly curved rod-shaped, nonmotile, non-spore-forming bacterium, strain Jip08(T), was isolated from rice straw in Daejeon, South Korea. Strain Jip08(T) produced colourless colonies (1.0-1.5 mm) on R2A medium after 3 days. On the basis of 16S rRNA gene sequence similarity, strain Jip08(T) was shown to belong to the alpha-2 subclass of the Proteobacteria, and was most closely related to Starkeya novella IAM 12100(T) (98.6%), Angulomicrobium tetraedale DSM 5895(T) (98.1%), Angulomicrobium amanitiforme NCIMB 1785(T) (98.0%), Ancylobacter aquaticus ATCC 25396(T) (96.6%) and Methylorhabdus multivorans DM13(T) (95.8%), all of which belong to the family Hyphomicrobiaceae. The genomic DNA G + C content of strain Jip08(T) was 69 mol%. Chemotaxonomic data [major ubiquinone, Q-10; major fatty acids, C-18:1 isomer (summed feature of C-18:1 omega 7c/omega 9t/omega 12t), C-19:0 cyclo omega 8c and C-16:0], 16S rRNA gene sequence analyses and some morphological and physiological features supported the affiliation of strain Jip08(T) to the genus Starkeya. However, DNA-DNA hybridization data and some phenotypic properties showed that strain Jip08(T) could be distinguished from the single known Starkeya species and represented a novel species, for which the name Starkeya koreensis sp. nov. is proposed. The type strain is Jip08(T) (= KCTC 12212(T) = NBRC 100963(T) = IAM 15215(T))

    Effects of oxidation on fatigue crack initiation and propafation in an advanced disk alloy

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    Understanding the fatigue performance of aeroengine disk alloys is important for both alloy development and life prediction of disk components. In this study, fatigue crack initiation and propagation in coarse grained (CG) and fine grained (FG) Low Solvus, High Refractory (LSHR) alloy developed for disk applications have been assessed at 650 and 725 oC by carrying out three-point bend tests in air and vacuum under a 1-1-1-1 trapezoidal waveform in combination with a surface replication procedure. Optical microscopy, scanning electron microscopy, focussed ion beam (FIB), transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) spectroscopy have been employed to reveal the underlying mechanisms of fatigue crack initiation and early propagation under these fatigue-oxidation conditions. The results show that FG LSHR possesses a better fatigue life although it exhibits more severe grain boundary oxidation as indicated by more intergranular fracture surfaces. Cracks mainly initiate from bulged grain boundary oxides which consist of outermost Co-rich and Ni-rich oxide complex at the surface and underneath Cr/Ti/Al oxide intrusions along the grain boundaries. Once initiated, cracks propagate mostly by the coalescence of neighboring grain boundary cracks as observed on the replicas, especially in the FG variant. Formation of these grain boundary oxides is closely related to the applied stress and the strain localization at the grain boundary, and is accompanied by ?? dissolution. The distribution of oxides examined by TEM-EDX suggests fatigue failure processes involve repeated cycles of oxide formation ? oxide cracking ? oxide formation at the crack tip ? oxide cracking at the crack tip

    Novel insoluble dye-labeled substrates for screening inulin-degrading microorganisms

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    Inulin hydrogels were used to prepare insoluble colored substrates for screening of inulin-degrading microorganisms. Methycrylated inulin, synthesized with glycidyl methacrylate, was crossed-linked by free radical polymerization producing an insoluble hydrogel. The inulin hydrogels were colored with one of three covalent dyes (Reactive blue 4, Reactive orange 14, Reactive red 120). The efficacy of the colored inulin hydrogels was determined by comparing their performance to insoluble, colored amylose, xylan and HE-cellulose in screening assays. Novel substrates (alone or in combination with other insoluble colored substrates) were successfully used for screening inulin-degrading microorganisms from sludge samples. Selected strains were identified using 16S rDNA gene partial sequencing; all of them belong to the genus Bacillus. (C) 2007 Elsevier B.V. All rights reserved
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