1,726,242 research outputs found
Iron responsive cell surface proteins in Thalassiosira pseudonana as probed by biotinylation and mass spectrometry
We investigated the cell-surface enriched proteome of Thalassiosira pseudonana under growth rate limiting (60-70% µ-max) and replete Fe conditions to better understand transporters that may be involved in Fe uptake. High and low Fe cultures were grown in the presence of 15N-NO3- (>98%) and 14N-NO3- (natural abundance), respectively, enabling relative quantification of proteins. In an effort to identify cell surface proteins, a cell surface labeling and enrichment method was developed and tested. Briefly, cell surface proteins were labeled with a free-amine reactive biotinylation reagent, soluble proteins were removed by membrane lysis and centrifugation, and biotinylated proteins were enriched on a neutravidin resin. Optimal conditions were sought for each of these three processes to increase coverage of cell surface labeled proteins. After elution, extracts were subjected to SDS-PAGE, in-gel tryptic digestion, and separation via liquid chromatography before identification and quantification by tandem mass spectrometry. Identification of cell surface proteins proved problematic due to biotinylation of some intracellular proteins and differential typtic digestion from the presence of the biotin linker arm. We obtained a greater than two-fold increase in abundance of the plasma membrane iron (III) permease (FTR1) under low Fe. A second FTR homolog was identified, indicating the presence of multiple Fe uptake pathways.M.S.Includes bibliographical referencesIncludes vitaby Ashley M. Ne
Predicting Chronic Absence in Jackson Public Schools
Poster Presenter: Ashley M. Perry
Research Team: izzy Thornton and Ashley M. Perry
Abstract: A Multi-Level Model in Support of a Plan to Reduce Chronic Absence in Jackson Public Schools (JPS). UM\u27s Center for Research Evaluation (CERE) is working in partnership with JPS, on a grant funded by the Kellogg Foundation, to explore various areas identified by the district as in need of improvement. This will include chronic absence rates, the effect of quality preK on kindergarten experience, and overall student outcomes
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
British and Jewish: Jewish Women???s Quest for Britishness and Jewish Civil and Political Equality in Britain, 1790-1860
Purpose of the Study:\ud
In my thesis, I examine the role of Jewish women in British acculturation and political\ud
and civil emancipation efforts in the first two-thirds of the nineteenth century. Histories\ud
written in the last hundred years on Jews in nineteenth-century Britain have generally not\ud
included Jewish women. British women's historians have done considerable research on\ud
women's roles and participation in nineteenth-century British politics and society in the\ud
last thirty years, but they too have predominantly ignored Jewish women. Anglo-Jewish\ud
literature scholars have done important research in the past twenty years, using Jewish\ud
women writers and their works to examine gender, religion, and identity in Victorian\ud
Britain. While their research has focused on Jewish women, Anglo-Jewish historians\ud
have yet to incorporate literature scholars??? sources or conclusions into their own works.\ud
By including Anglo-Jewish women and integrating them into the past, my research will\ud
begin to fill the existing gap in the fields of Modern Anglo-Jewish and British history.\ud
Procedure:\ud
To demonstrate that Jewish women in Britain participated in acculturation efforts and in\ud
the political and civil emancipation movement between 1790 and 1860, I particularly\ud
look at middle- and upper-class Anglo-Jewish women writers, such as Grace Aguilar and\ud
Celia and Marion Moss. I draw primarily on these women???s poems, novels, and\ud
travelogues, as well as newspaper articles, letters, journal entries, and other primary\ud
sources of the time, for my research and evidence. I have also used secondary sources,\ud
research done by other historians and literature scholars, to argue and prove my thesis.\ud
Findings:\ud
A large part of being British in 1790 or 1800 was being Anglican, but by 1830 elite\ud
Jewish men and women had gained British identities. They became socially and\ud
culturally indiscernible from Protestant Britons, while remaining Jewish. Like middle and\ud
upper-class Jewish men in Britain, Jewish women became British so they would gain\ud
all the rights and privileges of their class that came with Britishness, specifically\ud
citizenship and acceptance, which no Jews in Europe had at the time.\ud
What mattered more to Britons of the same economic class was wealth and culture, that\ud
Jews had the same amount of wealth and were culturally identical to them, rather than\ud
what religion they practiced. Evidence of that was elite non-Anglican Protestants and\ud
Catholics all having civil and political equality in Britain by 1830. They were considered\ud
British and included in the British polity, but despite their efforts, elite Jewish men and\ud
women were not. In order to be recognized as British by their government, elite Jewish\ud
women, along with Jewish men, took part in the Jewish emancipation movement and its\ud
debates on British identity and citizenship through written and public participation\ud
between 1830 and 1858. As a result of both sexes??? efforts, full British citizenship was\ud
granted to elite Jewish women and men in 1858, while Jewish men additionally gained\ud
full political rights.\ud
Conclusion:\ud
Middle- and upper-class Jewish women and men gained British identities befitting their\ud
economic class between 1790 and 1830 in order to be integrated into British society and\ud
culture and accepted by Christian Britons of their class. Anglo-Jews, both women and\ud
men, fought for inclusion, recognition, and acceptance in Britain between 1830 and 1858,\ud
and were successful in 1858. After all of their acculturation and emancipation efforts,\ud
elite Jews achieved Britishness, and were granted full civil and political rights as a result
Variations on the Author
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
The Relationship Between Leadership Characteristics Probation Officers Possess and the Success of Offenders
Recidivism continues to be a systematic issue within the United States. In recent years, criminal justice agencies have implemented evidence-based practices to help reduce recidivism rate and increase offender success within the communities. However, research suggests that recidivism rates have remained stagnant over the last few decades. The current study employed a linear regression analyses to determine what, if any, relationship exists between the leadership styles of probation officers and offender success (measured by recidivism rates). A significant positive relationship was observed between passive avoidant leadership style and recidivism rate. This result indicates that as level of passive avoidant leadership style increases, so does recidivism rate. The insights gained from this study led to the proposal of implementing a leadership survey instrument tool in the hiring/promotional process amongst U.S. Probation Offices throughout the nation.ProQuest Traditional Publishing Optio
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