1,720,959 research outputs found
In vitro induction of organogenesis and genetic transformation of cannabis (Cannabis sativa)
No full textVzpostavili smo uspešen protokol za gensko transformacijo konoplje (Cannabis sativa
L.). Za določitev protokola za adventivno regeneracijo konoplje smo izvedli več
poskusov. Najprej smo preizkusili različne regenerativne sposobnosti treh tipov
izsečkov (listnih pecljev, listnih izsečkov in internodijev) ter njihov potencial za tvorbo
kalusa. Raziskan je bil tudi učinek osnovnih gojišč DKW (Driver in Kuniyuki, 1984) in
MS (Murashige in Skoog, 1962). Predmet raziskave je bil tudi vpliv svetlobnega režima
na regenerativni potencial listnih pecljev in organogenetski potencial šestih genotipov
konoplje za uporabo v medicinske namene ter ene industrijske sorte konoplje.
Proučevan je bil tudi vpliv različnih koncentracij fitohormonov, kot so tidiazuron
(TDZ), 6-benzilaminopurin (BAP) in 1-naftalenocetna kislina (NAA), da bi našli najbolj
obetavno kombinacijo za razvoj kalusa in poganjkov. Listni peclji genotipa BF.KEM1.2
so imeli večji potencial za razvoj kalusa in poganjkov v primerjavi z drugima dvema
tipoma izsečkov. Vzpostavljena je bila adventivna regeneracija netransformiranih
poganjkov s 5 % uspešnostjo iz listnih pecljev genotipa BF.KEM3 na gojišču DKW z
0,2 mg/l TDZ, 0,5 mg/l NAA in 0,1 μM/l peptida fitosulfokin (PSK). Prisotnost svetlobe
je pozitivno vplivala na razvoj poganjkov in kalusa. Nadalje smo listne peclje iz
genotipa BF.KEM3 transformirali s kokultivacijo z bakterijo Agrobacterium
tumefaciens, ki je vsebovala plazmid pCAMBIA1302-ZsGreen, ki vsebuje v svoji regiji
T-DNA selekcijski gen hptII in reporterski gen ZsGreen. Prisotnost reporterskega gena
ZsGreen smo preverili z detekcijo zelene fluorescence in verižno reakcijo s polimerazo
(PCR) ter ga potrdili v 95 % pregledanih transformiranih kalusnih skupkih, prisotnost
gena hptII pa smo potrdili s PCR pri 85 % pregledanih transformiranih kalusnih skupkih.We have established a successful protocol for the genetic transformation of cannabis (C.
sativa L.). More stages of research were performed to establish the protocol for
adventitious regeneration of cannabis. We have first tested the different regenerative
potentials of three explant types (petioles, leaf cuttings, and internodes), and their
potential to produce callus. The effect of the DKW (Driver in Kuniyuki, 1984), and MS
(Murashige in Skoog, 1962) basic media was also explored. The influence of the light
regime on the regenerative potential of petioles, and the organogenetic potential of 6
medicinal genotypes one industrial cultivar of cannabis was also the subject of research.
The different concentrations of phytohormones thidiazurone (TDZ), 6-
benzylaminopurine (BAP), and 1-naphtaleneacetic acid (NAA), were also investigated
to find the most promising combination for callus production and shoot development.
Petioles from the BF.KEM1.2 genotype, showed the highest potential for callus and
shoots development compared with the other two explant types. Adventitious
regeneration of non-transformed shoots was also successfully established, with a 5%
rate of success, from the petioles of the BF.KEM3 genotype, on the DKW medium
supplemented with 0,2 mg/l of TDZ, 0,5 mg/l of NAA, and 0,1 μM/l of the
phytosulfokine (PSK) peptide. The presence of light positively influenced the shoots
and callus development. Furtherly, petioles from the genotype BF.KEM3 were
transformed by co-cultivation with Agrobacterium tumefaciens, which was carrying the
pCAMBIA1302-ZsGreen plasmid, which contains in its T-DNA region selection gene
hptII and reporter gene ZsGreen. We have confirmed the presence of the ZsGreen
reporter gene in 95 % of the examined transformed calli and the presence of the hptII
gene was confirmed in 85 % of the examined transformed calli
Transparency and Disclosure Practices in Selected Post-Transition Economies
Get PDFThe paper uses the methodology introduced by Standard & Poor’s to assess the level of transparency and disclosure exercised by the recently privatized companies from three Balkan countries: Croatia, Serbia and Macedonia. The procedure involves a thorough examination of the corporate websites to check for the presence of a list of 98 disclosure items. The results show that although the disclosure practices are gradually being improved, they still lag behind those of the older market economies. Additionally, the companies from the financial sector score considerably better than the average and the disclosure of management and governance data are the weakest point in each of the analysed countries. Companies with large shareholders owning sizable stakes are less prone to disclose. Preparation and publishing of classical annual reports is a rare practice and the companies seem to be more comfortable with disclosing data which are already made publicly available through other institutions. We find the reasons for this behaviour in the lack of relevance of the stock market information for the future of the companies and their management, which, in turn, has negative consequences for the development of the same stock markets. Keywords: corporate governance, transparency, disclosure, post-transition, privatization, Macedonia, Serbia, Croatia
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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