1,303 research outputs found
Author response: India and China in Africa: a comparative perspective of the oil industry by Raj Verma
No full textEarlier this month Ian Taylor reviewed India and China in Africa, a new book about Asian engagement in the West African oil industry. Here, the book’s author Raj Verma responds to Taylor’s comments, outlining the rationale and evidence for the framework used in the study. India and China in Africa: A comparative perspective of the oil industry. Raj Verma. London: Routledge. 2017
Single-Molecule Approaches to Stochastic Gene Expression
No full textBoth the transcription of mRNAs from genes and their subsequent translation into proteins are inherently stochastic biochemical events, and this randomness can lead to substantial cell-to-cell variability in mRNA and protein numbers in otherwise identical cells. Recently, a number of studies have greatly enhanced our understanding of stochastic processes in gene expression by utilizing new methods capable of counting individual mRNAs and proteins in cells. In this review, we examine the insights that these studies have yielded in the field of stochastic gene expression. In particular, we discuss how these studies have played in understanding the properties of bursts in gene expression. We also compare the array of different methods that have arisen for single mRNA and protein detection, highlighting their relative strengths and weaknesses. In conclusion, we point out further areas where single-molecule techniques applied to gene expression may lead to new discoveries.National Science Foundation (U.S.) (grant PHY-0548484)National Institutes of Health (U.S.) (grant R01-GM068957)National Institutes of Health (U.S.) (grant R01-GM077183)National Science Foundation (U.S.) (NSF Fellowship DMS-0603392)Burroughs Wellcome Fund (Career Award at the Scientific Interface
Interview with Lakshmi Raj Sharma, Author of The Tailor’s Needle
No full textInterview with Indian writer Lakshmi Raj Sharma, author of 'The Tailor's needle
Figures 19-21 in New records of jumping spiders (Araneae: Salticidae) from Nepal
No full textFigures 19-21. Three views of the female Telamonia dimidiata.Published as part of Magar, Kiran Thapa, Shrestha, Bimal Raj, Gurung, Tek Bahadur, K.C., Rabin Bahadur, Babu, Lamichhane, Ram, Hill, David E. & Thapa, Arjun, 2020, New records of jumping spiders (Araneae: Salticidae) from Nepal, pp. 1-11 in Peckhamia 220 (1) on page 9, DOI: 10.5281/zenodo.717125
Figures 14-15 in New records of jumping spiders (Araneae: Salticidae) from Nepal
No full textFigures 14-15. Two views of the male Phintella vittata.Published as part of Magar, Kiran Thapa, Shrestha, Bimal Raj, Gurung, Tek Bahadur, K.C., Rabin Bahadur, Babu, Lamichhane, Ram, Hill, David E. & Thapa, Arjun, 2020, New records of jumping spiders (Araneae: Salticidae) from Nepal, pp. 1-11 in Peckhamia 220 (1) on page 7, DOI: 10.5281/zenodo.717125
Figure 1 in New records of jumping spiders (Araneae: Salticidae) from Nepal
No full textFigure 1. Map of Chitwan National Park in Nepal, showing where each jumping spider species was found in the study area.Published as part of Magar, Kiran Thapa, Shrestha, Bimal Raj, Gurung, Tek Bahadur, K.C., Rabin Bahadur, Babu, Lamichhane, Ram, Hill, David E. & Thapa, Arjun, 2020, New records of jumping spiders (Araneae: Salticidae) from Nepal, pp. 1-11 in Peckhamia 220 (1) on page 2, DOI: 10.5281/zenodo.717125
FIGURE 2 in A new species of the genus Microhyla (Anura: Microhylidae) from Eastern Nepal
No full textFIGURE 2. Maximum likelihood tree based on DNA sequences of the 16s rRNA gene showing the phylogenetic relationships among 27 species of the genus Microhyla. Numbers present on branches of ML tree are bootstrap support values for Maximum likelihood (above) and Bayesian posterior probabilities (below) respectively. ML and BI values lower than 50% were represented with dash (–). Chaperina fusca was selected as an outgroup. Genbank accession number are presented in parenthesis. JRK refers to field collection number of Janak Raj Khatiwada and species with bold letters refer to Holotype.Published as part of Khatiwada, Janak Raj, Shu, Guo Cheng, Wang, Shou Hong, Thapa, Arjun, Wang, Bin & Jiang, Jianping, 2017, A new species of the genus Microhyla (Anura: Microhylidae) from Eastern Nepal, pp. 221-239 in Zootaxa 4254 (2) on page 224, DOI: 10.11646/zootaxa.4254.2.4, http://zenodo.org/record/54588
Variability in gene expression underlies incomplete penetrance
No full textThe phenotypic differences between individual organisms can often be ascribed to underlying genetic and environmental variation. However, even genetically identical organisms in homogeneous environments vary, indicating that randomness in developmental processes such as gene expression may also generate diversity. To examine the consequences of gene expression variability in multicellular organisms, we studied intestinal specification in the nematode Caenorhabditis elegans in which wild-type cell fate is invariant and controlled by a small transcriptional network. Mutations in elements of this network can have indeterminate effects: some mutant embryos fail to develop intestinal cells, whereas others produce intestinal precursors. By counting transcripts of the genes in this network in individual embryos, we show that the expression of an otherwise redundant gene becomes highly variable in the mutants and that this variation is subjected to a threshold, producing an ON/OFF expression pattern of the master regulatory gene of intestinal differentiation. Our results demonstrate that mutations in developmental networks can expose otherwise buffered stochastic variability in gene expression, leading to pronounced phenotypic variation.National Institutes of Health (U.S.). Pioneer AwardMathematical Sciences Postdoctoral Research Fellowships (DMS-0603392)National Institutes of Health (U.S.). Ruth L. Kirschstein National Research Service Award (5F32GM080966
- …
