58 research outputs found
Szacowanie prędkości pojazdów na podstawie obrazu z kamery = Estimating vehicle speed based on image from camera
No full textRajs Arkadiusz, Goździewska-Nowicka Agnieszka, Banaszak-Piechowska Agnieszka, Gospodarczyk Jacek. Szacowanie prędkości pojazdów na podstawie obrazu z kamery = Estimating vehicle speed based on image from camera. Journal of Education, Health and Sport. 2016;6(4):122-126. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.49880
http://ojs.ukw.edu.pl/index.php/johs/article/view/3456
The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).
755 Journal of Education, Health and Sport eISSN 2391-8306 7
© The Author (s) 2016;
This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License
(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial
use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 20.03.2016. Revised 17.04.2016. Accepted: 17.04.2016.
SZACOWANIE PRĘDKOŚCI POJAZDÓW NA PODSTAWIE OBRAZU Z KAMERY
ESTIMATING VEHICLE SPEED BASED ON IMAGE FROM CAMERA
dr inż. Arkadiusz Rajs
Uniwersytet Technologiczno-Przyrodniczy im. Jana i Jędrzeja Śniadeckich w Bydgoszczy, Wydział Telekomunikacji, Informatyki i Elektrotechniki,
Zakład Teletransmisji, Al. Prof. S. Kaliskiego 7, 85-790 Bydgoszcz, [email protected]
dr Agnieszka Goździewska-Nowicka
Uniwersytet Technologiczno-Przyrodniczy im. Jana i Jędrzeja Śniadeckich w Bydgoszczy, Wydział Zarządzania, Katedra Organizacji i Zarządzania,
ul. Fordońska 430, 85-790 Bydgoszcz, [email protected]
dr Agnieszka Banaszak-Piechowska
Uniwersytet Kazimierza Wielkiego w Bydgoszczy
Instytut Fizyki, ul. Weyssenhoffa 11, 85-090 Bydgoszcz, [email protected]
dr inż. Jacek Gospodarczyk
Wyższa Szkoła Gospodarki w Bydgoszczy
Instytut Informatyki i Mechatroniki
Garbary 2,85-229 Bydgoszcz, [email protected]
Streszczenie
Praca prezentuje sposób oszacowania prędkości poruszających się pojazdów.
Opisano metodę opartą na analizie obrazu oraz przedstawiono wyniki pomiarów.
Abstract
This Work presents a way to estimate the speed of the moving vehicles.
Describes a method based on image analysis, and shows the results of the measurements.
Słowa kluczowe: analiza obrazu, prędkość pojazdów, systemy wizyjne.
Key words: image analysis, machine vision systems, vehicle speed
Composite morphological structural element in the edge detecting
No full textRajs Arkadiusz, Aleksiewicz Mariusz, Goździewska-Nowicka Agnieszka, Parczyk Krystyna. Composite morphological structural element in the edge detecting. Journal of Education, Health and Sport. 2016;6(11):299-304. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.167138
http://ojs.ukw.edu.pl/index.php/johs/article/view/3996
The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).
755 Journal of Education, Health and Sport eISSN 2391-8306 7
© The Author (s) 2016;
This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License
(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial
use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 01.11.2016. Revised 12.11.2016. Accepted: 16.11.2016.
COMPOSITE MORPHOLOGICAL STRUCTURAL ELEMENT IN THE EDGE DETECTING
dr inż. Arkadiusz Rajs
Institute of Telecommunications and Computer Science, University of Science and Technology,
Al. Prof. S. Kaliskiego 7, 85-789 Bydgoszcz, [email protected]
dr inż. Mariusz Aleksiewicz
Institute of Telecommunications and Computer Science, University of Science and Technology,
Al. Prof. S. Kaliskiego 7, 85-789 Bydgoszcz,, [email protected]
dr Agnieszka Goździewska-Nowicka
University of Science and Technology, Faculty of Management,
ul. Fordońska 430, 85-790 Bydgoszcz, [email protected]
dr Krystyna Parczyk
WSB University in Toruń, ul. Młodzieżowa 31a, 87-100 Toruń, [email protected]
Summary. Edge detection is an important step in image analysis. Using mathematical morphology and individual structural elements can detect certain types of edges. But much remains undetected. This paper proposes edge detection algorithm based on the structural element made up of many individual structure elements. The experimental results are showing.
Key words: mathematical morphology, edge detectio
Flexible SERS platform based on PET/ITO/Ag for detection of pesticides
No full texthttps://kent-islandora.s3.us-east-2.amazonaws.com/node/14392/83903-thumbnail.jpgSurface-Enhanced Raman Spectroscopy (SERS) is a sensitive, non-destructive, rapid and powerful detection technique used for wide range of biological systems and chemicals analytes in low concentration and also as a diagnostic tool for environmental and biomedical analysis [1].To enhance the Raman signal the special SERS platform, usually made of silicon, glass or another brittle material, or metallic nanoparticles are required [2].
Due to extensive use of those chemical substances for plant fungicide and insecticide protection the detection and identification of pesticides on the surface of fruits and vegetables is a crucial issue. We show a new type of elastic SERS platform made of poly(ethylene terephthalate) (PET) covered with a layer of indium tin oxide (ITO). This composite is subjected to dielectric barrier discharge (DBD) that develops the active surface of the PET/ITO foil. To enhance the Raman signal, a modified composite was covered with a thin layer of silver using the physical vapor deposition (PVD) technique. The SERS platform was used for measurements popular pesticides, i.e., Thiram and Carbaryl and to quantitative analysis of those pesticides from fruits. The presented SERS platform exhibits excellent enhancement and reproducibility of the Raman signal, which enables the trace analysis of these pesticides in the range up to their maximum residues limit. [3].
REFERENCES
[1] Pilot R, Signorini R, Durante C, Bhamidipati DM, Fabris L., Biosensors (2019); 9, 57
[2] Mosier-Boss PA., Nanomaterials (2017);7,142
[3] Nowicka AB, Czaplicka M, Kowlaska AA, Szymborski T, Kamińska A., Biosensors (2019); 9,</p
Detection of circulating tumor cells (CTCs) by SERS-based immunomagnetic optofluidic device
No full texthttps://kent-islandora.s3.us-east-2.amazonaws.com/node/14388/83895-thumbnail.jpgThe isolation and characterization of circulating tumor cells (CTCs) has great potential for non-invasive biopsy. In this study, a surface-enhanced Raman spectroscopy (SERS) method was developed using magnetic nanoparticles and a solid SERS-active substrate integrated with an external field-assisted microfluidic device to efficiently isolate CTCs from blood samples. A new SERS substrate was used, developed by physically modifying the surface with a femtosecond laser, sputtering the active SERS layer and chemically modifying the surface with anti-EpCAM antibodies. Magnetic nanoparticles (Fe2O3) were coated with SERS active metal and then modified with para-mercaptobenzoic acid (p-MBA), which acts simultaneously as a Raman reporter and a linker with anti-EpCAM antibodies. The sensitive immune recognition of tumor cells is aided by the introduction of a controlled external magnetic field into the microfluidic chip. The integration of the SERS-active platform and p-MBA labeled immuno-Ag@Fe2O3 nanostructures with the microfluidic device ensures lower demand for samples and analytes, precise operation, increases the reproducibility of spectral responses and enables miniaturization and portability of the presented approach. We used four target tumor cell lines with relatively large (human prostate metastatic adenocarcinoma cells (LNCaP)), medium (adenocarcinomic human alveolar basal epithelial cells (A549)), weak (human prostate tumor line (PC3)) and no expression of EpCAM (tumor cells) cervical cancer (HeLa)) to estimate the detection limits on the basis of constructed calibration curves blood samples from lung cancer patients were used to validate the developed method.[1]
M. Czaplicka, K. Niciński, A. Nowicka, T. Szymborski i A. Kamińska, Cancers, 2020, 12 (3315), 1-21.
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Turystyczne wykorzystanie krajobrazu kulturowego w gminie Koronowo = Tourist use of the cultural landscape in Gmina Koronowo
No full textNowicka Ewa, Podgórski Zbigniew, Gonia Alicja. Turystyczne wykorzystanie krajobrazu kulturowego w gminie Koronowo = Tourist use of the cultural landscape in Gmina Koronowo. Journal of Education, Health and Sport. 2015;5(5):413-426. ISSN 2391-8306. DOI 10.5281/zenodo.18035
http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%285%29%3A413-426
https://pbn.nauka.gov.pl/works/562072
http://dx.doi.org/10.5281/zenodo.18035
Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011 – 2014 http://journal.rsw.edu.pl/index.php/JHS/issue/archive
Deklaracja.
Specyfika i zawartość merytoryczna czasopisma nie ulega zmianie.
Zgodnie z informacją MNiSW z dnia 2 czerwca 2014 r., że w roku 2014 nie będzie przeprowadzana ocena czasopism naukowych; czasopismo o zmienionym tytule otrzymuje tyle samo punktów co na wykazie czasopism naukowych z dnia 31 grudnia 2014 r.
The journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1089. (31.12.2014).
© The Author (s) 2015;
This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License
(http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial
use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 20.03.2015. Revised 18.04.2015. Accepted: 24.05.2015.
Turystyczne wykorzystanie krajobrazu kulturowego w gminie Koronowo
Tourist use of the cultural landscape in Gmina Koronowo
Ewa Nowicka, Zbigniew Podgórski, Alicja Gonia
Instytut Geografii, Wydział Kultury Fizycznej, Zdrowia i Turystyki, Uniwersytet Kazimierza Wielkiego, 85-428 Bydgoszcz, ul. Mińska 15
[email protected]
Słowa kluczowe: krajobraz kulturowy, turystyka, gmina Koronowo, metoda inwentaryzacji krajoznawczej, szlaki turystyczne.
Key words: cultural landscape, tourism, Gmina Koronowo, sightseeing inventory method, hiking trails.
Zarys treści
Gmina Koronowo jest powszechnie uznawana za teren atrakcyjnego krajobrazu, cenną i interesującą przestrzeń turystyczną. Predyspozycje obszaru do uprawiania różnych form turystyki potwierdza wynik oceny atrakcyjności wizualnej krajobrazu.
W artykule scharakteryzowano zasoby krajobrazu kulturowego gminy Koronowo. Uzyskane wyniki wskazują, że niemal każda miejscowość posiada obiekt o dużej wartości historycznej bądź kulturowej oraz walory krajoznawcze. Znaczna ich część znajduje się w Koronowie, z uwagi na wielkość miasta i jego bogatą historię. Powszechna opinia dowodzi, że Zalew Koronowski stanowi największy walor turystyczny gminy Koronowo. Nie ulega wątpliwości, iż jest on najmocniejszym atutem oraz marką turystyczną. Świadczy o tym m.in. wzrost intensywności ruchu turystycznego w sezonie letnim. Na podstawie wyników analizy SWOT stwierdzono, że gmina Koronowo ma nadal nie w pełni wykorzystany potencjał turystyczny. Fakt ten ze względu na przeciętną wielkość presji turystycznej potwierdza potrzebę dalszych działań na rzecz rozwoju turystyki na analizowanym obszarze.
Abstract
The gmina (third tier administrative unit) of Koronowo is widely regarded an area of attractive landscape, as well as valuable and interesting tourist space. Its suitability for various forms of tourism is confirmed by the assessment of visual attractiveness of the landscape. The article describes the resources of the cultural landscape of Gmina Koronowo. The results indicate that almost every locality has an object of high cultural or historical value as well as sightseeing potential. Most of those objects, however, are located in Koronowo itself, given the size of the town and its rich history. The common opinion proves that the Koronowo Reservoir is the biggest tourist value of Gmina Koronowo. Undoubtedly, it is the strongest asset and a tourist brand. This is evidenced by e.g. intensification of tourist traffic in the summer. Based on the results of the SWOT analysis it was found out that Gmina Koronowo is not fully exploiting its tourism potential. Due to the average size of the tourist pressure, this confirms the need for further action to develop tourism in the analysed area.
Translated by Aleksandara Zaparucha.Nowicka Ewa, Podgórski Zbigniew, Gonia Alicja. Turystyczne wykorzystanie krajobrazu kulturowego w gminie Koronowo = Tourist use of the cultural landscape in Gmina Koronowo. Journal of Education, Health and Sport. 2015;5(5):413-426. ISSN 2391-8306. DOI 10.5281/zenodo.18035
http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%285%29%3A413-426
https://pbn.nauka.gov.pl/works/562072
http://dx.doi.org/10.5281/zenodo.1803
Defective interaction between Pol2p and Dpb2p, subunits of DNA polymerase epsilon, contributes to a mutator phenotype in Saccharomyces cerevisiae.
No full textMost of the prokaryotic and eukaryotic replicative polymerases are multi-subunit complexes. There are several examples indicating that noncatalytic subunits of DNA polymerases may function as fidelity factors during replication process. In this work, we have further investigated the role of Dpb2p, a noncatalytic subunit of DNA polymerase epsilon holoenzyme from Saccharomyces cerevisiae in controlling the level of spontaneous mutagenesis. The data presented indicate that impaired interaction between catalytic Pol2p subunit and Dpb2p is responsible for the observed mutator phenotype in S. cerevisiae strains carrying different mutated alleles of the DPB2 gene. We observed a significant correlation between the decreased level of interaction between different mutated forms of Dpb2p towards a wild-type form of Pol2p and the strength of mutator phenotype that they confer. We propose that structural integrity of the Pol epsilon holoenzyme is essential for genetic stability in S. cerevisiae cells
Dielectrophoresis-Based SERS Sensors for the Detection of Cancer Cells in Microfluidic Chips
No full textThe detection of freely circulating cancer cells (CTCs) is one of the greatest challenges of modern medical diagnostics. For several years, there has been increased attention on the use of surface-enhanced Raman spectroscopy (SERS) for the detection of CTCs. SERS is a non-destructive, accurate and precise technique, and the use of special SERS platforms even enables the amplification of weak signals from biological objects. In the current study, we demonstrate the unique arrangement of the SERS technique combined with the deposition of CTCs cells on the surface of the SERS platform via a dielectrophoretic effect. The appropriate frequencies of an alternating electric field and a selected shape of the electric field can result in the efficient deposition of CTCs on the SERS platform. The geometry of the microfluidic chip, the type of the cancer cells and the positive dielectrophoretic phenomenon resulted in the trapping of CTCs on the surface of the SERS platform. We presented results for two type of breast cancer cells, MCF-7 and MDA-MB-231, deposited from the 0.1 PBS solution. The limit of detection (LOD) is 20 cells/mL, which reflects the clinical potential and usefulness of the developed approach. We also provide a proof-of-concept for these CTCs deposited on the SERS platform from blood plasma
Machine Learning for COVID-19 Determination Using Surface-Enhanced Raman Spectroscopy
No full textThe rapid, low cost, and efficient detection of SARS-CoV-2 virus infection, especially in clinical samples, remains a major challenge. A promising solution to this problem is the combination of a spectroscopic technique: surface-enhanced Raman spectroscopy (SERS) with advanced chemometrics based on machine learning (ML) algorithms. In the present study, we conducted SERS investigations of saliva and nasopharyngeal swabs taken from a cohort of patients (saliva: 175; nasopharyngeal swabs: 114). Obtained SERS spectra were analyzed using a range of classifiers in which random forest (RF) achieved the best results, e.g., for saliva, the precision and recall equals 94.0% and 88.9%, respectively. The results demonstrate that even with a relatively small number of clinical samples, the combination of SERS and shallow machine learning can be used to identify SARS-CoV-2 virus in clinical practice
Photopolymer-Based Composite with Substance Release Capability Manufactured Additively with DLP Method
No full textIn this study, caffeine-loaded photoresin composites with homogeneous structures, suitable for additive manufacturing of transdermal microneedle systems, were obtained. The properties of the composites with varying caffeine concentrations (0.1–0.4% w/w) were investigated for carbon–carbon double bond conversion using Fourier Transform Infrared Spectroscopy, surface wettability and mechanical properties using a static tensile test and nanoindentation, and caffeine release in ethanol using UV-Vis. The caffeine concentration did not affect the final degree of double bond conversion, which was confirmed in tensile tests, where the strength and Young’s modulus of caffeine-loaded samples had comparable values to control ones. Samples with 0.1 and 0.2% caffeine content showed an increase in nanohardness and reduced elastic modulus of 50 MPa and 1.5 MPa, respectively. The good wettability of the samples with water and the increase in surface energy is a favorable aspect for the dedicated application of the obtained composite materials. The amount of caffeine released into the ethanol solution at 1, 3 and 7 days reached a maximum value of 81%, was higher for the lower concentration of caffeine in the sample and increased over time. The conducted research may enhance the potential application of composite materials obtained through the digital light processing method in additive manufacturing
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