294 research outputs found
My Early Days with Ari Helenius: Detergents and Viruses.
No full textThis Transit Authority commentary is an account of how Ari Helenius started his research on membranes, viruses and detergents
Microsoft Powerapps -ohjelman soveltuvuus matka- ja kululaskutuksen mobiilisovelluksen luomiseen : toiminnallinen toteutus Digia Oyj:lle
No full textTässä opinnäytetyössä tarkastellaan mobiilisovelluksen luomista Microsoft PowerApps -virtuaalialustalla, joka ei vaadi aiempaa ohjelmointiosaamista. Tutkimus on toteutettu luomalla matka- ja kululaskujen syöttöön tarkoitettu mobiilisovellus. Matka- ja kululaskut syötetään mobiilisovelluksella web service -rajapinnan yli Microsoft D365 Business Central -toiminnanohjausjärjestelmän tietokantaan jatkokäsittelyä varten.
Työssä tutkimus on tehty toiminnallisena työnä hyödyntäen kirjallisuus- ja verkkomateriaalia, asiantuntijahaastatteluita ja vertailuanalyysia. Opinnäytetyön laatija on työskennellyt asiantuntijatehtävissä 2018 alkaen työn toimeksiantajayrityksessä, Digia Oyj:ssä.
Haastattelut on tehty opinnäytetyön kohdeyrityksen työntekijöille. Niissä tarkennetaan PowerApps-sovelluksen tarpeita ja sen tuomia mahdollisuuksia, ja verrataan niitä työssä tehtyihin havaintoihin. Vertailuanalyysi toteutuu luomalla vastaava mobiilisovellus suoraan toiminnanohjausjärjestelmän käyttöliittymään.
Tutkimuksen tuloksina syntyi matka- ja kululaskujen sovellus Microsoft PowerAppsilla ja Microsoft Business Centralilla toteutettuna. Näiden sovellusten kehitysten pohjalta on kirjattu päätelmät teknillisestä ja kaupallisesta näkökulmasta, vertailu asiakkaan ja sovellustoimittajan hyödyistä sekä sovelluksen jatkokehitysideat.In this thesis is inspected creating a mobile software with Microsoft PowerApps virtual platform which requires no prior programming experience. Study is done by creating a mobile software for feeding travel expense and bill of service entries. The entries are inserted over web service API to Microsoft D365 Business Central ERP’s database for further handling.
Research has been done through a practical implementation by utilizing book and web material, specialist interviews and comparison analysis. The author of the thesis has worked as a specialist from 2018 onwards in the case company, Digia Plc.
Interviews are done for the case company’s employees. Questions are to pinpoint needs and opportunities of PowerApps and to compare them to observations done by the author. Comparison analysis is done by creating a similar software UI in the ERP.
As a result of the research, a software for travel expense and bill of service was made with Microsoft PowerApps and Microsoft D365 Business Central. Conclusions have been written on the basis of the software development from technical and commercial aspects, comparison of customer’s and vendor’s benefits and further development ideas for the software
Virus entry at a glance
No full textThe goal of a virus particle is to transport its genome in a replication-competent form from an infected cell to an uninfected cell. To enter a new host cell, the majority of viruses take advantage of the endocytic mechanisms of the cell and wait until reaching endocytic vacuoles or other cytoplasmic compartments before penetrating into the cytosol. After penetration, viruses and viral capsids exploit the cytoplasmic transport systems of the cell, moving to sites of replication within the cytosol (most RNA viruses) or the nucleus (most DNA viruses). The final step in the entry process generally involves uncoating of the viral genome.After numerous entry studies with a variety of viruses in different cell systems, the overall picture of entry of animal virus into the host cell is becoming increasingly complete. There are essentially six locations for penetration. These are: (1) the plasma membrane, (2) the early endosome, (3) the maturing endosome, (4) the late endosome, (5) the macropinosome and (6) the endoplasmic reticulum (ER). Endolysosomes, amphisomes and lysosomes also remain possible penetration sites, but evidence is lacking. Some viruses can use more than one pathway, for example, by engaging different receptors.Although the molecular details of virus–cell surface interactions are complex and highly variable, the number of pathways that allow the viruses to reach their sites of penetration seem to be limited to a handful of endocytic mechanisms. The subsequent trafficking steps involve key organelles in the endocytic network that are utilized by endogenous cargo. Only a few viruses seem to be capable of penetrating directly through the cell surface by fusing their envelope with the plasma membrane. Thus, the ‘trail map’ that is available to incoming viruses is complex, but far from incomprehensible.In this Cell Science at a Glance article, we describe the stepwise entry program of animal viruses of different families. As illustrated in the poster, the process can be broken down into five discrete steps – attachment, signaling, endocytosis, penetration and uncoating. A more detailed description can be found in previous reviews (Damm and Pelkmans, 2006; Greber, 2002; Grove and Marsh, 2011; Gruenberg, 2009; Marsh and Helenius, 2006; Mercer and Helenius, 2009; Mercer et al., 2010; Schelhaas, 2010; Sieczkarski and Whittaker, 2002)
Folding, assembly and secretion of tick-borne encephalitis virus envelope proteins in mammalian cells
No full textComparison of the cellular requirements for macropinocytosis, VV, IAV CME-independent, and HPV-16 endocytosis, and phagocytosis.
No full text<p>Depicted are characteristics and required factors for macropinocytosis, phagocytosis, and endocytic entry of VV, IAV, and HPV-16. Tick marks indicate that a factor is required, crosses indicate no requirement, both indicate a cell type/system dependend requirement, and n.d. = not determined. Marked with an asterisk (*) are differences between macropinocytosis and HPV-16. The data is from this manuscript and (Mercer and Helenius, 2008, Mercer and Helenius 2009, de Vries et al., 2011, Niedergang and Chavrier, 2005).</p
Plant-based production of Cyanovirin-N, a cyanobacterial protein with anti-HIV properties
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