196,570 research outputs found
The Testament of Orpheus, Aristobulus, and the Derveni Papyrus: Between "Didactic" Hymnography and Alexandrian Exegesis
No full textStarting from the analysis of The Testament of Orpheus as it is preserved by Aristobulus-Eusebius, this essay aims at demonstrating that the version attributed to the Hellenic-Jewish philosopher illustrates a theological and cosmological doctrine similar to the cosmosophia of the pseudo-Aristotelian treatise De mundo. In this paper the Author concludes also that the wider version of the Testament of Orpheus would actually be contemporary to Aristobulus. The Author recommends also to consider Aristobulus as the exegete of a previous/more or less contemporary hymn who attempts to lead the sense of this towards a more explicit ‘Aristotelian’ direction (as it emerges from De mundo) by adopting an exegetical practice largely used in Alexandria but already found in the historicizing allegoresis proposed in the Derveni Papyrus. About this preexisting hymn, Aristobulus (or the source he re-read and commented) has provided further elements that were somehow ascribable to Orpheus, perhaps interpreting the references to the figure of a son (almost certainly a collective or a bequest of a formulary of testamentary origin used in the Psalms and in some readings from Second
Temple Judaism) as allusions to Musaeus, the Orpheus’s son. The passage mentioned by Aristobulus is not very clear (how could Musaeus’s subjection to Orpheus be explained since in other Jewish-Hellenic contexts Musaeus is considered to be Orpheus’s guide and/or father? Moreover, Musaeus is also clearly identified as the son of the shining moon). Instead, it is a fact that in the subsequent Christian tradition, where Musaeus belongings to Orpheus’s lineage appears as more solidified, there emerges an explicit identification of the persona loquens of the hymn with the protos heuretes of Greek poetry, a figure who is traditionally remembered as author of hymns
Valine 114 Replacements in Archaeal Elongation Factor 1A Enhanced Its Ability To Interact with Aminoacyl-tRNA and Kirromycin
No full textValine 114 in the D109AAILVVA sequence of elongation factor 1R from the archaeon Sulfolobus solfataricus (SsEF-1R) was substituted with an acidic (V114E), basic (V114K), or cavity-forming (V114A) residue, and the effects on the biochemical properties of the factor were investigated. This sequence is well-conserved among most of eukaryal and eubacterial counterparts, and in the three-dimensional structure of SsEF-1R, V114 is located in a hydrophobic pocket near the first GDP-binding consensus sequence G13XXXXGK[T,S] [Vitagliano, L., Masullo, M., Sica, F., Zagari, A., and Bocchini, V. (2001) EMBO J. 20, 5305-5311]. These mutants displayed functions absent in the wild-type factor. In fact, although they exhibited a rate in poly(Phe) incorporation almost identical to that of SsEF-1R, V114K and V114A exhibited an affinity for GDP and GTP higher and a capability to bind heterologous aa-tRNA stronger than that elicited by SsEF-1R but similar to that of eubacterial EF-Tu. V114E instead displayed not only a weaker binding capability for aa-tRNA but also a lower affinity for GDP. The intrinsic GTPase activity of V114E was drastically reduced compared to those of SsEF-1R, V114K, and V114A. Interestingly, the decreased intrinsic GTPase activity of V114E was partially restored by kirromycin, an effect already observed for the G13A mutant of SsEF-1R [Masullo, M., Cantiello, P., de Paola, B., Catanzano, F., Arcari, P., and Bocchini, V. (2002) Biochemistry 41, 628-633]. Finally, the V114A substitution showed only a marginal effect on both the thermostability and thermophilicity of SsEF-1R, whereas V114K and V114E replacements strongly destabilized the molecule
A NAD(P)H oxidase isolated from the archaeon Sulfolobus solfataricus is not homologous with another NADH oxidase present in the same microorganism
No full textA NAD(P)H oxidase has been isolated from the ar- chaeon Sulfolobus solfataricus. The enzyme is a ho- modimer with Mr 38,000 per subunit (SsNOX38) contain- ing 1 FAD molecule/subunit. It oxidizes NADH and, less efficiently, NADPH with the formation of hydrogen per- oxide. The enzyme was resistant against chemical and physical denaturating agents. The temperature for its half-denaturation was 93 and 75 °C in the absence or presence, respectively, of 8 M urea. The enzyme did not show any reductase activity. The SsNOX38 encoding gene was cloned and sequenced. It accounted for a prod- uct of 36.5 kDa. The translated amino acid sequence was made of 332 residues containing two putative -fold regions, typical of NAD- and FAD-binding proteins. The primary structure of SsNOX38 did not show any homol- ogy with the N-terminal amino acid sequence of a NADH oxidase previously isolated from S. solfataricus (Ss- NOX35) (Masullo, M., Raimo, G., Dello Russo, A., Boc- chini, V. and Bannister, J. V. (1996) Biotechnol. Appl. Biochem. 23, 47–54). Conversely, it showed 40% sequence identity with a putative thioredoxin reductase from Ba- cillus subtilis, but it did not contain cysteines, which are essential for the activity of the reductase
Response to letter from Madias regarding our article “Admission heart rate and in-hospital course of patients with Takotsubo syndrome”
No full textWe thank you for your stimulating letter [1], commenting on our ar- ticle recently published in the Journal [2].
Tracking heart rate (HR) changes after admission represents an at- tractive matter of investigation. Indeed, it can be presumed that persis- tently elevated HR in patients with Takotsubo syndrome (TTS) during hospital stay may represent an unfavorable indicator, similarly to per- sistent ST-segment elevation [3]. Unfortunately, some limitations apply [1] and beta-blockers administration in the early phases could represent a main confounder in a population initially treated mostly as having acute coronary syndrome. Nevertheless, this is an interesting issue to be addressed in future prospective studies.
We observed 11 patients (5%) presenting with HR b 60 beats per minute, and none had developed bradycardia after admission. However, time from symptoms onset to presentation largely varies, and it cannot be excluded that, as described in animal models of subarachnoid hem- orrhage [4], bradycardia at hospital arrival may represent a following step in the pathogenic pathway of TTS.
⁎ Correspondingauthorat:ViadiGrottarossa1035/1039,00189Rome,Italy. E-mail address: [email protected] (L. Arcari).
1 Drs. Arcari and Limite contributed equally to this letter.
https://doi.org/10.1016/j.ijcard.2018.09.077
0167-5273/© 2018 Elsevier B.V. All rights reserved.
Finally, a more accurate and close monitoring of the autonomic sym- pathetic nervous system activity is needed to deeply understand the mechanisms involved in TTS pathophysiology, and surely the suggested new available technology [5] would be helpful. However, for this purpose, the long delay with which TTS patients usually come to medical attention after the onset of symptoms remains source of problems, preventing us from witnessing the very early events and leaving us to record mainly later clinical manifestations. Unfortunately, no technology can shade light on this “blind phase” of TTS
Gesù come "(il) figlio dell'/d’uomo". "Parole-azioni", "veridizioni storiche" e flussi di trasmissione tra l’Apocalisse di Giovanni e i sinottici.
No full textLo studio è dedicato all’analisi dei rapporti tra (alcuni de)i detti sul figlio dell’uomo presenti nei Vangeli sinottici e le visioni che hanno come protagonista un personaggio designato come tale nell’Apocalisse di Giovanni. L’indagine, che in parte continua quella proposta nel volume Visioni del figlio dell’uomo nel Libro delle Parabole e nell’Apocalisse (Morcelliana, Brescia 2012), ma che si concentra sullo specifico dei rapporti tra i vangeli sinottici e l’Apocalisse (aspetto che nella monografia del 2012 era soltanto accennato per lasciare spazio ad altri flussi di trasmissione protocristiani), mette a frutto la prospettiva metodologica di M. Foucault tesa a valorizzare le differenze tra le cosiddette “parole- azioni” e “resoconti aleturgici” o “racconti di veridizione storicizzata” nei contesti religiosi e più generalmente culturali tra antichità e tardo-antichità. Proprio partendo dalle riflessioni di Foucault sui discorsi, l’articolo tenta di valorizzare la possibile preistoria dei materiali protocristiani che mettono al centro della loro ricostruzione memoriale di Gesù la figura del figlio dell’/d’uomo danielico, risalendo alle pratiche visionarie che stanno dietro all’Apocalisse, e postulando una loro anteriorità o maggiore aderenza alle prassi dei primissimi gruppi di credenti in Gesù (almeno nella trasmissione di specifici flussi sul figlio dell’/d’uomo) rispetto ai resoconti storicizzanti dei sinottici. Lo studio cerca anche di testare i risultati emersi dall’indagine monografica condotta dall'autore nel 2012 sulla base di alcune nuove ricerche, in particolare le ultime condotte da L.W. Hurtado e quelle di M. Vinzent
Memorie, flussi di trasmissione, coabitazioni
No full textStarting from the recent book by A. Destro and M. Pesce, Il racconto e la scrittura (Rome, Carocci, 2015), this article endeavors to explain the differences and contradictions between the Gospels in the light of the fact that their authors were dependent on information dating back to groups of followers of Jesus who were located in different parts of the Land of Israel and who did not have contact with one another
Neuronal aspects and plasticity of inferior olivary complex and nucleus dentatus
No full textIn primary or physiological ageing, the brain undergoes a 10% weight reduction. This datum was confirmed in vivo by radiological investigation and post mortem by morphological research (Grandi and Coll. 1990, 1990, 1991, 1991), that underscores the same reduction in the medulla oblongata and in the cerebellum. Morphological and cytohistological aspects of primary ageing have been considered for the inferior olivary nucleus and for the dentate nucleus in 41 male subjects, 32 belonging to the decades 7th, 8th, 9th, and 9 to the decades from the 3rd to the 6th. From the two latter structures, separated along standard cut surfaces, serial histological slices were carried out, and histochemical reactions were accomplished, together with immunohistochemistry, for synaptophysin, NF-2F11, NSE, S-100, GFAP, chromogranin. As the weight progressively decreased from the 7th to the 9th decade, it was noted neuronal loss, both in the inferior olivary nucleus, and in the dentate nucleus. Furthermore, the loss appeared to be followed by substitutive astroglial proliferation. Immunocytochemical reaction for synaptophysin revealed peculiar aspects. A consistently regular result of such method concerned the two nuclei studied, in primary ageing, thus allowing the interpretation of the perfect structure of synaptic vesicles as an indirect argument for neuronal plasticity
The organization of lymphatic and blood vessels in the Peyer's patches of the small intestine and colon
No full textThe migration of lymphocytes in the interfollicular lymphatic vessels of the intestinal Peyer's patches
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