1,720,992 research outputs found
Functional analysis of the African swine fever virus MGF360 and MGF505 proteins, and its application to vaccine development
Get PDFAfrican swine fever virus (ASFV) is a deadly haemorrhagic pathogen of swine. In recent years, ASFV has spread beyond Africa and continues to ravage European and Asian pig herds, posing a severe socio-economic threat. No vaccines are currently available leaving preventive and extermination measures as the only course of action. The virus’ dsDNA genome is up to 190kb in length, and contains 5 clusters of paralogous genes known as Multigene family (MGF) genes close to both termini. Genes of MGF360 and MGF505 clusters have been implicated in the subversion of the innate immune system, and their absence is known to attenuate virulent isolates, however their specific mechanism of action is unknown. Here I have characterised the functions of MGF360-10L, 11L, 12L, 13L, 14L, MGF505-1R, 2R and 3R, by performing in silico and in vitro analysis. I revealed that these MGF genes inhibit the interferon signalling pathways at the level of the transcription factors or below. MGF360-12L and MGF505-1R were demonstrated to both contain a functional novel Cullin-RING-E3 ubiquitin ligase (CRL) recruitment motif, which was indispensable for the inhibitory action of MGF505- 1R on the interferon pathway. Moreover MGF505-1R reduced levels of endogenous p300, a cofactor essential in the transcription of type-I Interferon and other genes. Mutagenesis of the CRL motif restored p300 levels, indicating that MGF505-1R targets the p300 cofactor for CRL driven ubiquitination and subsequent proteasomal degradation. I applied the knowledge gained here to the rational development of vaccine candidates. Two profiles of MGF genes were deleted from the virulent Georgia07/1, and the impact on ex vivo ISG expression, and in vivo attenuation in pigs was assessed. This revealed the specific genes potentially key for attenuation. Overall, I have advanced knowledge on understanding the molecular mechanisms of the ASFV MGF360 and MGF505 proteins and identified two of those with critical roles during virus infection
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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