228 research outputs found
Microdomain-specific beta-adrenergic regulation of calcium signaling in tachycardia-induced atrial fibrillation
No full textTo our knowledge, no studies have been performed to investigate microdomain-specific protein kinase A (PKA)-dependent-phosphorylation of ryanodine receptors (RyR) during '-adrenergic modulation in atrial fibrillation (AF). Therefore, atrial myocytes from sham-operated- (SHAM) and rapid atrial paced (RAP; 5 days at 10 Hz) rabbits were stimulated under baseline or after '-adrenergic stimulation (Isoproterenol, 300 nM). Ca2+ transients were measured confocally during field stimulation (1 Hz, 37 °C). PKA-dependent RyR phosphorylation was analyzed by immunostaining and assigned to the nearest membrane. Confocal images were analyzed using ImageJ. Statistical significance (p<0.05) was evaluated with Student's t-test, Mann-Whitney U test, or ANOVA. At global level in atrial RAP cells, amplitude of Ca2+ transients and RyR phosphorylation were significantly reduced at baseline, but normalized after '-adrenergic stimulation. At microdomain level in atrial RAP cells, '-adrenergic rescue of RyR phosphorylation involved equal recruitment of RyR at uncoupled, subsarcolemmal and axial regions. In atrial myocytes, level of PKA-dependent RyR phosphorylation depends on the subcellular location. Atrial remodeling due to rapid pacing causes RyR hypophosphorylation that can be reversed by '-adrenergic stimulation. This mechanism could, at least partly, contribute to the '-adrenergic rescue of Ca2+ transients in AF improving contractility, but could adversely increase the likelihood of arrhythmias
Microdomain-specific beta-adrenergic regulation of calcium signaling in tachycardia-induced atrial fibrillation
No full textTo our knowledge, no studies have been performed to investigate microdomain-specific protein kinase A (PKA)-dependent-phosphorylation of ryanodine receptors (RyR) during '-adrenergic modulation in atrial fibrillation (AF). Therefore, atrial myocytes from sham-operated- (SHAM) and rapid atrial paced (RAP; 5 days at 10 Hz) rabbits were stimulated under baseline or after '-adrenergic stimulation (Isoproterenol, 300 nM). Ca2+ transients were measured confocally during field stimulation (1 Hz, 37 °C). PKA-dependent RyR phosphorylation was analyzed by immunostaining and assigned to the nearest membrane. Confocal images were analyzed using ImageJ. Statistical significance (p<0.05) was evaluated with Student's t-test, Mann-Whitney U test, or ANOVA. At global level in atrial RAP cells, amplitude of Ca2+ transients and RyR phosphorylation were significantly reduced at baseline, but normalized after '-adrenergic stimulation. At microdomain level in atrial RAP cells, '-adrenergic rescue of RyR phosphorylation involved equal recruitment of RyR at uncoupled, subsarcolemmal and axial regions. In atrial myocytes, level of PKA-dependent RyR phosphorylation depends on the subcellular location. Atrial remodeling due to rapid pacing causes RyR hypophosphorylation that can be reversed by '-adrenergic stimulation. This mechanism could, at least partly, contribute to the '-adrenergic rescue of Ca2+ transients in AF improving contractility, but could adversely increase the likelihood of arrhythmias
The role of microdomains in regulating ryanodine receptors (RyRs) and the alterations after myocardial infarction in cardiomyocytes
No full textstatus: Publishe
LATAR DALAM KINDERROMAN “OMA! SCHREIT DER FRIEDER” KARYA GUDRUN MEBS
No full textABSTRAK
NINDIA NOVIANTI, 2020. Latar dalam Kinderroman “Oma! Schreit der Frieder” Karya Gudrun Mebs. Skripsi, Jakarta : Program Studi Pendidikan Bahasa Jerman, Fakultas Bahasa dan Seni, Universitas Negeri Jakarta.
Tujuan dari penelitian ini adalah untuk megetahui latar yang terdapat dalam Kinderroman “Oma! Schreit der Frieder“ karya Gudrun Mebs sebagai salah satu cara untuk menambah pemahaman mahasiswa tentang karya sastra, khususnya dalam bentuk prosa, yaitu Kinderroman. Penelitian ini menggunakan metode kualitatif dengan teknik studi pustaka.
Fokus penelitian ini yaitu latar dalam Kinderroman “Oma! Schreit der Frieder“ karya Gudrun Mebs. Data dalam penelitian ini adalah kalimat yang menggambarkan latar dalam Kinderroman, yang dianalisis berdasarkan jenis latar menurut teori Claus Gigl. Bedasarkan hasil analisis ditemukan 6 jenis latar yang dalam Kinderroman Oma! Schreit der Frieder karya Gudrun Mebs, yaitu Handlungsraum sebanyak 34 data, Stimmungsraum 30 data, Lebensraum 9 data, Gedankenraum 6 data, Symbolraum 4 data dan Kontrastraum 1 data. Latar yang paling banyak ditemukan adalah Handlungsraum, karena pengarang ingin lebih menunjukan suatu peristiwa sebagai tempat atau lokasi kegiatan tokoh beraktivitas.
Berdasarkan analisis tersebut, penelitian ini diharapkan dapat digunakan sebagai materi tambahan untuk membantu pemahaman mahasiswa Program Studi Pendidikan Bahasa Jerman mengenai latar terutama dalam Kinderroman.
Kata Kunci: Latar, Kinderroman, Oma! Schreit der Frieder, Gudrun Mebs
ABSTRACT
NINDIA NOVIANTI, 2020. The Setting in Kinderroman "Oma! Schreit der Frieder" by Gudrun Mebs. Thesis, Jakarta: German Language Education Study Program, Faculty of Language and Art, Jakarta State University.
The purpose of this study is to find out the setting contained in the "Oma! Schreit der Frieder" by Gudrun Mebs as one way to increase students' understanding of literary works, especially in the form of prose, namely Kinderroman. This study uses qualitative methods with literature study techniques.
The focus of this research is the setting in the Kniderroman "Oma! Schreit der Frieder" by Gudrun Mebs. The data in this study are sentences that describe the setting in Kinderroman, which are analyzed based on the type of setting according to Claus Gigl's theory. Based on the results of the analysis found 6 types of background in Oma! Schreit der Frieder by Gudrun Mebs, namely Handlungsraum with 34 data, Stimmungsraum 30 data, Lebensraum 9 data, Gedankenraum 6 data, Symbolraum 4 data and Kontrastraum 1 data. The most common setting is Handlungsraum, because the author wants to show an event more as a place or location for activities of a character.
Based on this analysis, this research is expected to be used as additional material to help students understand the German Language Study Program regarding background, especially in Kinderroman.
Keywords: Setting, Kinderroman, Oma! Schreit der Frieder, Gudrun Mebs
On the Representation of ‘Worst Case Scenarios’ in Kafka’s Short Stories “Metamorphosis” and “A Hunger Artist’’
No full textIn occasione del centenario della morte di Franz Kafka, il presente documento affronta la questione del perché questo autore appartenga ancora agli scrittori più affascinanti del mondo di lingua tedesca. Oltre agli aspetti generali della ricezione letteraria della sua opera, vengono esaminate questioni fondamentali riguardanti il rapporto tra rappresentazione letteraria e realtà. In questo contesto, l'attenzione si concentra su due dei suoi racconti più letti, “Metamorfosi" (Die Verwandlung) e “Il digiunatore" (Ein Hungerkünstler). L'approccio teorico è integrato da una sezione più storica e descrittiva, in cui vengono presentate la biografia e le opere di Kafka. Dopo una valutazione degli approcci interpretativi alle due opere selezionate per la nostra analisi, ci concentriamo in particolare sulla descrizione progressiva delle catastrofi. La sequenza degli eventi negativi per i protagonisti descritti nei racconti di Kafka viene sistematicamente registrata ed elaborata per fornire ulteriori argomenti che caratterizzano Kafka oggi, a cento anni dalla sua morte, come un importante autore moderno.On the occasion of the 100th anniversary of Franz Kafka's death, this document presents some reasons why this author is still one of the most fascinating writers in the German-speaking world. The document examines the general principles of Franz Kafka's literary work together with some fundamental questions such as the relationship between literary representation and reality in two of his most-read stories, “Metamorphosis” and “A Hunger Artist”. Some important aspects of literary reception are considered and the theoretical approach is complemented by a more historical and descriptive section, in which Kafka's biography and his works (literary history) are presented. The focus is on the evaluation and interpretative approaches to the two works selected for our analysis, in particular on the progressive description of catastrophes (literary criticism). The sequence of negative events for the protagonists described in Kafka's stories are systematically recorded and processed to provide additional arguments that characterize Kafka today, one hundred years after his death, as an important modern author
Repolarization variability and early afterdepolarizations in long QT syndrome type 2: is labile calcium the common denominator?
No full textThe progressive ankyloses protein ANK facilitates clathrin- and adaptor-mediated membrane traffic at the trans-Golgi network-to-endosome interface.
No full textThis is a pre-copyedited, author-produced version of an article accepted for publication in Human molecular genetics following peer review. The version of record Wenke Seifert, York Posor, Peter Schu, Gudrun Stenbeck, Stefan Mundlos, Sabine Klaassen, Peter Nürnberg, Volker Haucke, Uwe Kornak, Jirko Kühnisch, The progressive ankylosis protein ANK facilitates clathrin- and adaptor-mediated membrane traffic at the trans-Golgi network-to-endosome interface, Human Molecular Genetics, Volume 25, Issue 17, 1 September 2016, Pages 3836–3848, is available online at: https://doi.org/10.1093/hmg/ddw23
Review of: Gudrun-Axeli Knapp: Im Widerstreit. Feministische Theorie in Bewegung. Wiesbaden: Springer VS 2012.
No full textGudrun-Axeli Knapp hat mit ihrem Sammelband eine beeindruckende und überaus umfangreiche Werkschau aus 25 Jahren feministischer Theoriebildung vorgelegt. Der Band ist in vier thematische Blöcke unterteilt, die um Weiblichkeitskritik, feministische Aneignungen der Kritischen Theorie, Intersektionalität und die Frage nach der Gestalt feministischer (theoretischer) Kritik kreisen. Dabei überzeugt die Autorin nicht nur durch ihre sorgfältigen wie streitbaren Argumentationen und Auseinandersetzungen im Terrain feministischer Theorie; reizvoll gestaltet sich überdies das Nachspüren jener Denkfigur über die Einzelbeiträge des Sammelbands hinweg, die auch den Titel des Bandes prägt: „im Widerstreit“. Damit legt Knapp auch Umrisse einer Programmatik feministischer Kritik vor.With her collection, Gudrun-Axeli Knapp presents an impressive and exceptionally extensive retrospective on 25 years of feminist theorizing. The volume is subdivided into four subject areas, which deal with femininity criticism, feminist appropriations of critical theory, intersectionality, and the question of the nature of feminist (theoretical) criticism. The author does not only impress with her thorough yet also provocative argumentations and examinations of the terrain of feminist theory; but the figure of thought that she traces across all articles and that coins the collection’s title is also very appealing: “in conflict”. In doing so, Knapp does also outline aims and objectives of feminist criticism
Mechanosensitivity of microdomain calcium signalling in the heart
No full textIn cardiac myocytes, calcium (Ca2+) signalling is tightly controlled in dedicated microdomains. At the dyad, i.e. the narrow cleft between t-tubules and junctional sarcoplasmic reticulum (SR), many signalling pathways combine to control Ca2+-induced Ca2+ release during contraction. Local Ca2+ gradients also exist in regions where SR and mitochondria are in close contact to regulate energetic demands. Loss of microdomain structures, or dysregulation of local Ca2+ fluxes in cardiac disease, is often associated with oxidative stress, contractile dysfunction and arrhythmias. Ca2+ signalling at these microdomains is highly mechanosensitive. Recent work has demonstrated that increasing mechanical load triggers rapid local Ca2+ releases that are not reflected by changes in global Ca2+. Key mechanisms involve rapid mechanotransduction with reactive oxygen species or nitric oxide as primary signalling molecules targeting SR or mitochondria microdomains depending on the nature of the mechanical stimulus. This review summarizes the most recent insights in rapid Ca2+ microdomain mechanosensitivity and re-evaluates its (patho)physiological significance in the context of historical data on the macroscopic role of Ca2+ in acute force adaptation and mechanically-induced arrhythmias. We distinguish between preload and afterload mediated effects on local Ca2+ release, and highlight differences between atrial and ventricular myocytes. Finally, we provide an outlook for further investigation in chronic models of abnormal mechanics (eg post-myocardial infarction, atrial fibrillation), to identify the clinical significance of disturbed Ca2+ mechanosensitivity for arrhythmogenesis. (C) 2017 Published by Elsevier Ltd
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