33,028 research outputs found
Stinklepus Stinkleby Takes A Bath
No full textThis book was donated to BU by its author Stephanie Angelini, 2005 College of Communication alumna. All e-book versions of this work display best on the widest margin settings and the largest font setting. You may download the free Kindle reading software here: http://is.gd/yg9P5k
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The author has permitted us to make the book available to the BU community only. To download the files, please click on the appropriate lock icon and log in with your BU credentials. Thank you.Meet Stinklepus Stinkleby, in this beautifully illustrated bedtime story. He sniffles, snuffles, and snorts. He looks like mixture of a bunny, a bear, a kitty, and even a racoon, but to his Doggie-Mommie, Doggie-Sister, and Doggie-Daddy, he is 100% dog. He likes to eat, he likes to sleep, and he loves to chase his toys. But one thing he does not like at all, ever, or under any circumstances, is taking a bath!
When he overhears his family say, "Stinklepus Stinkleby, you stink!", he knows that bath time is near. But if they want to wash this stinky little dog, they'll have to catch him first.
A great read for bath time, bedtime, or anytime
Ugo Maria Ugazio, Il problema della morte nella filosofia di Heidegger
No full textAngelini Antoine. Ugo Maria Ugazio, Il problema della morte nella filosofia di Heidegger. In: Revue Philosophique de Louvain. Quatrième série, tome 80, n°48, 1982. pp. 704-705
Handheld-Impedance-Measurement System with seven-decade capability and potentiostatic function
No full textThis paper describes design and test of a new impedance-measurement system for nonlinear devices that exhibits a seven-decade range and works down to a frequency of 0.01 Hz. The system is specifically designed for electrochemical measurements, but the proposed architecture can be employed in many other fields where flexible signal generation and analysis are required. The system employs an unconventional signal generator based on two pulsewidth modulation (PWM) oscillators and an autocalibration system that allows uncertainties of less than 3% to be obtained over a range of 1 kΩ to 100 GΩ. A synchronous demodulation processing allows the noise superimposed to the low-amplitude input signals to be made negligibl
Maria Bersani
No full textLa voce illustra la biografia e l'apporto letterario dato da Maria Bersani alla letteratura per l'infanziaThe headword explains the biography and the contribution of the author Maria Bersani to the children's literatur
Exposure-Tolerant Imaging Solution forCultural Heritage Monitoring
No full textThis paper describes a simple and cheap solution specifically designed for monitoring the degradation of thin coatings employed for metal protection. The proposed solution employs a commercial photocamera and a frequency-domain-based approach that is capable of highlighting the surface uniformity changes due to initial corrosion. Even though the proposed solution is specifically designed to monitor the long-time performance of protective coatings employed for the restoration of silver artifacts, it can be successfully used also for assessing the conservation state of other ancient metallic works of art. The proposed solution is made tolerant to exposure changes by using a procedure for sensor nonlinearity identification and correction, does not require a precise lighting control, and employs only free open-source software, so that its overall cost is very low and can be used also by not specifically trained operator
Título: Vida de la venerable, extatica y virgen, Maria Vitoria Angelini Romana, congregante de la Virgen Santissima de los Dolores
No full textLista de personas insignes de la Congregacion de la Virgen Santissima de los Dolores y diversos HimnosExiste un estado (A) con errores en el cuaderno 4[adorno tipográfico]\b3-4\sExiste un estado (B) con un retrato diferente de Maria Vittoria Angelini no firmadoSign.: 2*\p4\s, 3[adorno tipográfico]-4[adorno tipográfico]\p4\s, 5[adorno tipográfico]\p2\s, A-T\p4\s, []\p4\s, V-Z\p4\s, 2A-2E\p4\s, 2F\p8\s, 2G-2Z\p4\s, 3A-3P\p4\s, [cristus]\p4\s, 2[cristus] ¶\p1\s, ¶\b2-4\sError de paginación, de página 464 pasa a 455Cada parte con portada propiaAmbas portadas a dos tintas, roja y negraTexto con apostillas marginales y reclamosLetras iniciales enmarcadas y viñetas xilográficasLas hojas de grabados calcográficos: "Randon sculp. Massiliae"Contiene : Primera parte, Vida de ... Ana Juliana Gonzaga de Cleves, Archiduquessa de Austria ..., de la ... tercera regla de los Siervos de Maria / recopilada de diversos autores por ... Narcisso Galindo, religioso de los Siervos de Maria ... ; Segunda parte, Vida de ... Maria Vitoria Angelini Romana, Congregante de la Virgen Santissima de los Dolores / compuesta en italiano por ... Juan Bautista Pacichelli ..., traducida en castellano por ... Narciso Galindo, religioso servita ..
Studies of cytotoxicity on human tumor cells induced by enzymatic oxidation products of polyamines: a new approach in cancer therapy
No full textBackground
The polyamines spermine, spermidine and putrescine are ubiquitous cell components and required for several physiological functions, such as DNA synthesis, cell growth and differentiation. Polyamine concentrations are high in growing tissues such as tumors. Amine oxidases are important because they contribute to the regulation of polyamines’ levels. These enzymes catalyze the oxidative deamination of polyamines to generate the reaction products H2O2 and aldehyde(s), that are able to induce oxidative stress in several cultured human tumor cell lines. Because of the limited success of chemotherapy for tumors, most investigations have oriented on cellular and molecular mechanisms responsible for the onset of multidrug resistance (MDR). Several studies have therefore been performed to overcome the MDR phenotype and to develop innovative chemotherapeutic strategies effective against MDR tumors. Our previous studies using bovine serum amine oxidase (BSAO) have shown that H2O2 and aldehyde(s) were responsible for the cytotoxicity induced in human colon adenocarcinoma cells (LoVo). The treatment of tumors with BSAO could be useful as a new strategy for cancer therapy. In this study, maize polyamine oxidase (ZmPAO), a flavin adenine dinucleotide (FAD)-dependent enzyme, was used due to both its high enzymatic specific activity and the low molecular weight, which are important aspects for the delivery of these enzymes into cancer cells. I investigated on the cytotoxicity and the mechanism of cell death induced by enzymatic oxidation product of polyamine generated by ZmPAO.
Methods
Subconfluent LoVo WT cells, its doxorubicin resistance (MDR) cells, and AGS gastric tumor cells were used to investigate the cytotoxic effect induced by polyamine metabolites. The harvested tumor cells were treated with exogenous spermine and ZmPAO purified from maize shoot at 37°C up to 1 hr. The treated cells were used in the following assays. Cell viability assays were performed by clonogenic assay. After the treatment, cells were incubated at 37°C for 10-17 days until colonies were formed. The colonies were stained and counted. The involvement of apoptotic cell death was assessed by Annexin V-FITC/PI staining and cell cycle, using flow cytometry. An increasing of the sub-G1 peak was detected. In order to determine the involvement of mitochondria in apoptosis, we also examined the loss of mitochondrial membrane potential using JC-1. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations were performed on LoVo cells to examine the morphological changes and the ultrastructure modifications, respectively, after the treatment.
Results and discussion
Spermine and ZmPAO treatment reduced the number of colonies in a spermine dose-dependent manner and a time-dependent manner in LoVo and AGS cells. I also found that the treatment further decreased the cell viability in LoVo MDR cells when compared with LoVo WT cells. Moreover, there was no difference in cell viability after the treatment with spermine and spermidine in both LoVo WT and MDR cells, suggesting that aminoaldehydes derived from spermine and spermidine do not have any cytotoxic effect, it is mainly due to H2O2. Further colony formation assay revealed that cytotoxicity induced by spermine metabolites was completely prevented by the addition of exogenous catalase in both LoVo WT and MDR cells, while it was not affected by the presence of exogenous ALDH. Moreover, LoVo cells exposed to exogenous H2O2 exhibited a slightly higher cytotoxicity than that induced by ZmPAO and spermine. The concentration of H2O2 actually generated by ZmPAO from spermine might be lower than that of H2O2 when directly exposed. Similar results were also obtained in AGS cells, showing that the effect of cell growth inhibition induced by polyamines and ZmPAO was not cell specific.
Treatment with ZmPAO and spermine at 42°C showed an increase in cytotoxicity on LoVo and AGS cells when compared with the cells treated at 37°C. Cytotoxicity was higher in LoVo MDR cells than in their WT counterpart, and it is probably due to a synergistic effect of spermine metabolites at 42°C.
Annexin V-FITC and PI staining assay showed that the treatment with ZmPAO and spermine increased the percentage of apoptotic cells population in a spermine dose-dependent manner in LoVo WT. The treatment also increased the cell population at the sub-G1 phase that is characteristic of apoptosis. In both assays, the proportion of apoptotic cells was higher in LoVo MDR than in LoVo WT.
SEM analysis showed a significant number of membrane blebs after the treatment with ZmPAO and spermine in LoVo MDR cells, while LoVo WT cells showed minor morphological changes. TEM observation further revealed that the treatment exhibited the typical ultrastructural features of apoptosis, including vacuolization in the cytoplasm and mitochondrial alterations. In particular, in MDR cells, I found severe vacuolization and cell membrane blebbing.
The involvement of mitochondria in the cytotoxicity was supported by JC-1 assay. It showed that the exposure of LoVo cells to ZmPAO and spermine reduced mitochondrial membrane potential, which was greater in LoVo MDR cells than in WT cells, in agreement with the results of cell viability and apoptosis assays.
We demonstrated that ZmPAO and spermine treatment induced cytotoxicity through an apoptotic pathway, which was more pronounced in LoVo MDR cells compared to LoVo WT ones. Since polyamine levels are up-regulated in tumor sites and the development of MDR tumor cells is recognized as a major problem, the design of a new therapeutic strategy based on the use of this combination in human tumors might be taken into account, making this approach mainly attractive in treating MDR cancer patients
La didattica della rappresentazione. G. Monge, M.J Sganzin, Annibale Angelini, Francesco Paolo Tucci.
No full textL'autrice delinea il ruolo del disegno nel percorso formativo degli ingegneri attraverso quattro approfondimenti monografici su Gasperd Monge, M.J. Sganzin, Annibale Angelini, Francesco Paolo Tucci.
Attraverso tali figure e la loro opera si consolida il ruolo di medium grafico e le specifiche connotazioni assunte dal disegno nel percorso formativo degli ingegneri napoletani in relazione al più ampio contesto culturale "europeo
Frammenti di Futuro. Alessandro Anselmi e la cultura dell'Architettura a Roma
No full textConferenza “Frammenti di Futuro” organizzata dal Dottorato di Ricerca in Architettura - Teorie e Progetto, coordinato dal Professor Antonino Saggio, in occasione del trigesimo della scomparsa di Alessandro Anselmi, avvenuta il 28 gennaio 2013.
Sono intervenuti gli architetti Franco Purini, Antonino Saggio, Lucio Altarelli, Maria Argenti, Valter Bordini, Francesco Cellini, Paolo Grassi, Francesco Montuori, Marcello Pazzaglini, Paolo Portoghesi, Pierluigi Serraino, Mario Spada, Valentino Anselmi, Valerio Palmieri e i dottorandi di ricerca in Architettura Teorie e Progetto Domenico Ferrara, Carla Molinari, Anna Riciputo, Eride Caramia, Gaetano De Francesco, Erika Maresca, Selena Anders, Rosetta Angelini, Leopoldo Russo Ceccotti, Stefano Bigiotti, Raphaela Papaléo Farias a cura di Rosetta Angelini, Eride Caramia, Carla Molinari.
Gli atti della conferenza sono pubblicati nel volume Alessandro Anselmi Frammenti di futuro
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