55 research outputs found
Mapping research on knowledge management in family firms: a bibliometric analysis
No full textPurpose – Given the growing interest in the topic of knowledge management (KM) in family firms (FFs) and the subsequent increasing number of papers published, this study aims to review the field to identify and analyze the main themes and trends.
Design/methodology/approach – This study applies bibliometric techniques to a sample of 146 papers published from 2007 to 2023 and their 8,126 unique cited references. Bibliometric coupling is performed on the sample papers to explore the current intellectual structure of the field of KM in FFs, whereas cocitations analysis is performed to investigate the different literature streams that served as roots for the development of such a field.
Findings – Bibliographic coupling reveals that sample papers can be grouped into four clusters, and, through papers content analysis, the author identifies their core themes as knowledge sharing, innovation, knowledge-based dynamic capabilities and intellectual capital. Cocitation analysis of the cited references revealed four main clusters that can be considered the literature streams that served as roots for the development of the field, i.e. knowledge-based view, socioemotional wealth, strategic
management and social capital (as a theory and as a resource).
Originality/value – This study contributes to the literature on KM in FFs by extending prior systematic review efforts with bibliometric analyses and combining these results to highlight connections between the main research themes around which scholars have debated (i.e. the clusters identified through bibliometric coupling) and their theoretical foundations (i.e. the clusters identified through cocitation analysis). This study also has practical implications by synthesizing and informing managers about FFs’
advantages and weaknesses in the KM process
Determination of Gymnemic Acid I as a Protein Biosynthesis Inhibitor Using Chemical Proteomics
No full textThe plant Gymnema sylvestre has been used widely in traditional medicine as a remedy for several diseases, and its leaf extract is known to contain a group of bioactive triterpene saponins belonging to the gymnemic acid class. Gymnemic acid I (1) is one of the main components among this group of secondary metabolites and is endowed with an interesting bioactivity profile. Since there is a lack of information about its specific biological targets, the full interactome of 1 was investigated through a quantitative chemical proteomic approach, based on stable-isotope dimethyl labeling. The ribosome complex was found to be the main partner of compound 1, and a full validation of the proteomics results was achieved by orthogonal approaches. Further biochemical and biological investigations revealed an inhibitory effect of 1 on the ribosome machinery
Functional Proteomics discloses the biological targets of natural compounds
No full textFunctional Proteomics discloses the biological targets of natural compound
Multi-target profile of oleocanthal, an extra-virgin olive oil component
No full textThis review is a brief but comprehensive overview on the multi-target profile of oleocanthal, an extra-virgin olive oil (EVOO) phenol corresponding to the (-)-decarboxymethylligstroside aglycone, speculating about its potential in the prevention and/or treatment of various diseases, such as neurodegeneration, inflammation and cancer
Chemical proteomics of natural compounds useful for the development of nutraceutics.
No full textChemical proteomics of natural compounds useful for the development of nutraceutics
Multi-target profile of oleocanthal, an extra-virgin olive oil component
No full textThis review is a brief but comprehensive overview on the multi-target profile of oleocanthal, an extra-virgin olive oil (EVOO) phenol corresponding to the (-)-decarboxymethylligstroside aglycone, speculating about its potential in the prevention and/or treatment of various diseases, such as neurodegeneration, inflammation and cancer
The essential player in adipogenesis GRP78 is a novel KCTD15 interactor
No full textKCTD15 is a member of the K+ Channel Tetramerization Domain family, implicated in crucial physio-pathological processes. Recent evidences suggest that KCTD15 is an obesity-linked protein in humans and its Drosophila homologue is involved in food uptake. KCTD15 molecular mechanism in these processes is still unknown. To fill this gap, KCTD15 was biophysically characterized showing a folded, pentameric region endowed with a remarkable thermal stability. Notably, the C-terminal domain significantly contributes to the stabilization of the BTB N-terminal domain. The availability of large amount of stable recombinant protein also made possible a functional proteomic approach in 3T3-L1 cells to search for novel KCTD15 interactors. These investigations led to the discovery that GRP78 is a KCTD15 partner in all the adipogenesis phases. Our data clearly prove the physical interaction of the two proteins and also indicate that GRP78 plays an active role in the stabilization of KCTD15. Furthermore, the presence in Drosophila of a GRP78 homologue corroborates the physiological role played by the complex KCTD15-GRP78 in the adipogenesis process and indicates that it is evolutionarily conserved. Present results also suggest that KCTD15 may be a new target for obesity control
Proteasome as a new target for bio-inspired benzo[k,l]xanthene lignans
No full textMass spectrometry-based chemical proteomics is a powerful tool in the target discovery of small molecules. Here we present the application of this approach to define the target profile of bio-inspired synthetic benzo[k,l]xanthene lignans, endowed with interesting biological properties. Proteasome has been identified as a new main interactor for this class of compounds. A combination of molecular docking and in vitro and in cell fluorescence assays gave insights on the molecular mechanism of interaction, highlighting the the attitude of these lignans to inhibit the proteasome
An investigation of direct and indirect demand for transport and logistic services in transition countries: the case of Albania
No full textProteomic analysis of Zn depletion/repletion in the hormone-secreting thyroid follicular cell line FRTL-5
No full textZinc deficiency predisposes to a wide spectrum of chronic diseases. The human Zn proteome was predicted to represent about 10% of the total human proteome, reflecting the broad array of metabolic functions in which this micronutrient is known to participate. In the thyroid, Zn was reported to regulate cellular homeostasis, with a yet elusive mechanism. The Fischer Rat Thyroid Cell Line FRTL-5 cell model, derived from a Fischer rat thyroid and displaying a follicular cell phenotype, was used to investigate a possible causal relationship between intracellular Zn levels and thyroid function. A proteomic approach was applied to compare proteins expressed in Zn deficiency, obtained by treating cells with the Zn-specific chelator N,N,N′,N′-tetrakis (2-pyridylmethyl) ethylene-diamine (TPEN), with Zn repleted cells. Quantitative proteomic analysis of whole cell protein extracts was performed using stable isotope dimethyl labelling coupled to nano-ultra performance liquid chromatography-mass spectrometry (UPLC-MS). TPEN treatment led to almost undetectable intracellular Zn, while decreasing thyroglobulin secretion. Subsequent addition of ZnSO4 fully reversed these phenotypes. Comparative proteomic analysis of Zn depleted/repleted cells identified 108 proteins modulated by either treatment. Biological process enrichment analysis identified functions involved in calcium release and the regulation of translation as the most strongly regulated processes in Zn depleted cells
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