136 research outputs found
Identification of novel genes involved in phosphate accumulation in Lotus japonicus through Genome Wide Association mapping of root system architecture and anion content
No full text130 Lotus japonicus accessions were used. The names and accession numbers are
listed in S6 Table. Seeds were scarified with sandpaper and then sterilized 14 minutes in 0.05%
sodium hypochlorite. Subsequently, seeds were rinsed and washed 5 times in sterile distilled
water. For the germination, seeds were positioned in imbibed filter paper, in sterile Petri dishes,
and wrapped in aluminium foil. After 3 days at 21°C, young seedling were transferred to square
plates (12 x 12 cm) containing growth medium. Both media used in this
study were based on Long-Ashton solution (with two levels of phosphate concentration -20 or
750 μM, LP or HP, respectively) with 0.8% MES buffer (Duchefa Biochemie,
Haarlem, The Netherlands), 0.8% agarose (to minimize phosphate contamination), and adjusted
to pH 5.7 with 1M KOH. After adding the medium, plates were dried, closed, overnight in a
sterile laminar flow hood. Two accessions, with four replicates per each accession, were placed
on each plate. Each plate was replicated, with mirrored position of each accession to minimize
any positional growth effects. Plates were placed vertically, and plants grown under long-day
conditions (21°C, 16 h light/8 h dark cycle) with white light bulbs emitting 50 μmol/m 2 /s and
roots were exposed to light. Every day at the same time, the racks were transported to the image
acquisition room where images of each plate were acquired with eight Epson V600 CCD flatbed
color image scanners (Seiko Epson) and then immediately returned to the growth chamber.</p
Mobile technologies: expectancy, usage and acceptance of clinical staff
No full textAlbrecht U-V, Illiger K, Jungnickel T, von Jan U. Mobile technologies: expectancy, usage and acceptance of clinical staff. In: Lovis C, Séroussi B, Hasman A, Pape-Haugaard L, Saka O, Kjær Andersen S, eds. e-Health – For Continuity of Care. Studies in Health Technology and Informatics. Vol 205. Amsterdam: IOS Press; 2014: 1221
Draft Guideline for the Development of Evidence Based Medicine-Related Apps
No full textGonnermann A, von Jan U, Albrecht U-V. Draft Guideline for the Development of Evidence Based Medicine-Related Apps. In: Cornet R, Stoicu-Tivadar L, Hörbst A, Parra Calderón CL, Kjær Andersen S, Hercigonja-Szekeres M, eds. Digital Healthcare Empowering Europeans. Studies in Health Technology and Informatics. Vol 210. Amsterdam: IOS Press; 2015: 637-641.Evidence based recommendations can significantly aid decision processes in medicine and mobile apps are starting to enter this domain. Considering the rapid access to and quick processing of information made possible by such apps, it is especially important to ensure the quality and structure of the provided data and to also keep the limitations of the information sources in mind. A draft guideline meant for implementing appropriate standards for such apps is outlined in this contribution
Narrative threads: ethnographic tourism, Romani tourist tales, and fiber art
No full textThis thesis examines the need for the ethnographer to process their own emotions and experiences as part of the ethnographic experience. Specifically, it argues for the credibility of artistic expression resulting from fieldwork.
Drawing on the author’s experience during the 2012 inaugural "Romani Music, Culture, and Human Rights" study abroad program at the University of Pittsburgh, this thesis offers an analysis of five works of fiber art. Originally perceived by the author as separate from the thesis writing process, they became an integral part of thesis once they were recognized as the non-verbal processing of the my emotional response to events abroad and, therefore, essential components of the research process.
I argue that emotional processing is an integral part of writing an ethnography, for as the ethnographer works through their experiences, their understanding of the events changes, and this in turn impacts the ways in which the ethnographic is perceived and analyzed
Stig Andersen m.fl., FN og U-landene. Internationalt udviklingssamarbejde - organisationer og projekter, København: FN-Forbundet, 1979, 132 s., kr. 30,
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Evaluation of a Diagnostic Reasoning Program (DxR): Exploring Student Perceptions and Addressing Faculty Concerns
No full textAbstract: Clinical reasoning is essentially a problem-solving process, in which medical students must learn to gather and interpret data, generate hypotheses and make decisions. To develop skills in problem-solving it is argued that students need more tools, rather than more answers (Masys, 1989). DxR is a computerised case series, in which students use 'doctor tools' to investigate a patient problem. This report describes a pilot evaluation of DxR in fourth year medicine at the University of Sydney. It addresses faculty concerns regarding the program, explores student perceptions, and looks at the capacity of the program to stimulate and support the development
of clinical reasoning skills. It finally discusses possibilities for using DxR to support learning in medicine.
Reviewers: Stig Andersen (Virtual Centre for Health Informatics, Aalborg, DK), David Good (U. Cambridge), Timothy Koschmann (U. Colorado)
Interactive elements: 'Details are provided for obtaining a DxR demonstration CD.'
DxR is distributed by NOVARTIS, although the DxR cases described in this article are not the same as those listed in the
Diagnosis category of their bookshop/website. A Macromedia Director demonstration of DxR can be obtained by contacting Tanaya Patel, Project Director,
DxR Development Group ([email protected]). Alternatively, a demonstration CD, and complimentary catalogue of patient cases and price list can be obtained by calling 800-631-1181 (USA)
Maternal hypothyroidism and adverse outcomes of pregnancy
No full textObjective: Hypothyroidism has been associated with pregnancy complications, but uncertainty prevail regarding the severity and the role of thyroid autoimmunity. This study aimed to evaluate adverse pregnancy outcomes by exposure to maternal hypothyroidism and thyroid autoimmunity. Design: Retrospective cohort study. Patients: 14,744 singleton pregnancies from the North Denmark Region Pregnancy Cohort (2011−2015). Measurements: Maternal thyroid stimulating hormone (TSH), thyroid peroxidase antibodies (TPO-Ab), and thyroglobulin antibodies (Tg-Ab) were retrospectively measured in early pregnancy blood samples (ADVIA Centaur XPT, Siemens Healthineers). Adjusted odds ratio (aOR) with 95% confidence interval (CI) was used to estimate associations between maternal hypothyroidism (TSH cut-offs: 6.0 and 10 mIU/L), thyroid autoimmunity (TPO-Ab cut-off: 60 U/ml, Tg-Ab cut-off: 33 U/ml), and adverse pregnancy outcomes. Results: Pregnancy outcomes were 93.2% live births, 6.5% spontaneous abortions, and 0.3% stillbirths. The frequency of spontaneous abortion was 6.5% when TSH was below 6.0 mIU/L, 6.5% when above 6.0 mIU/L (aOR 1.0 [95% CI: 0.5−2.0]), and 12.5% when above 10 mIU/L (aOR: 2.0 [95% CI: 0.8−5.2]). For outcome of preterm birth, the frequency was 5.4% when TSH was below 6.0 mIU/L, 7.8% when above 6.0 mIU/L (aOR 1.5 [95% CI: 0.7−2.9]), and 11.4% when above 10 mIU/L (aOR: 2.6 [95% CI: 0.9–7.3]). No association was found between thyroid autoantibodies and spontaneous abortion (TPO-Ab: aOR: 1.0 [0.8−1.3], Tg-Ab: 1.0 [0.8−1.2]) or preterm birth (TPO-Ab: aOR: 1.0 [0.8–1.2], Tg-Ab: 0.9 [0.7–1.2]). Conclusion: A high frequency of adverse pregnancy outcomes was seen among pregnancies exposed to maternal TSH above 10 mIU/L, whereas no association with thyroid autoantibodies was seen.</p
Phenotyping revealed tolerance traits and genotypes for acidity and aluminum toxicity in European Vicia faba L
No full textSoil acidity is a global issue; soils with pH <4.5 are widespread in Europe. This acidity adversely affects nutrient availability to plants; pH levels <5.0 lead to aluminum (Al3+) toxicity, a significant problem that hinders root growth and nutrient uptake in faba bean (Vicia faba L.) and its symbiotic relationship with Rhizobium. However, little is known about the specific traits and tolerant genotypes among the European faba beans. This study aimed to identify response traits associated with tolerance to root zone acidity and Al3+ toxicity and potentially tolerant genotypes for future breeding efforts. Germplasm survey was conducted using 165 genotypes in a greenhouse aquaponics system. Data on the root and shoot systems were collected. Subsequently, 12 genotypes were selected for further phenotyping in peat medium, where data on physiological and morphological parameters were recorded along with biochemical responses in four selected genotypes. In the germplasm survey, about 30% of genotypes showed tolerance to acidity and approximately 10% exhibited tolerance to Al3+, while 7% showed tolerance to both. The phenotyping experiment indicated diverse morphological and physiological responses among treatments and genotypes. Acid and Al3+ increased proline concentration. Interaction between genotype and environment was observed for ascorbate peroxidase activity, malondialdehyde, and proline concentrations. Genomic markers associated with acidity and acid+Al3+-toxicity tolerances were identified using GWAS analysis. Four faba bean genotypes with varying levels of tolerance to acidity and Al3+ toxicity were identified.Peer reviewe
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