1,721,244 research outputs found
Morphology and Permeability of Exfoliated PVAc-MMT Nanocomposite Films Cast From Soap-Free Emulsion-Polymerized Latices
From Erasmus to Maius:The History of Codex Vaticanus in New Testament Textual Scholarship
An-Ting Yi writes an extensive scholarly history of the famous Codex Vaticanus over the course of 350 years. Currently regarded as one of the most essential pillars to reconstruct the Greek New Testament text, the perceptions of this manuscript have changed profoundly in history, from an inferior witness to the most valuable foundation. This work uncovers how it became Codex Vaticanus par excellence in New Testament textual scholarship
Fabrication of PEO17?OPV3 Templated Titania Nano-Hollow Rods and Their Aggregating Microspheres
Crosslinkable Poly(vinyl acetate)/Clay Nanocomposite Films Cast from Soap-Free Emulsion-Polymerized Latices
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Identification of white spot syndrome virus latency-associated genes
近年來,在一般草蝦養殖池之草蝦白點症病毒檢測結果顯示,約90%為白點症病毒帶原蝦。潛伏性病毒感染屬於一種病毒與寄主長期共存的關係,病毒潛伏狀態的維持與少數病毒基因之表現有關。本研究目的在於鑑定並確定蝦白點症病毒潛伏性相關基因的存在。針對白點症病毒532個開放譯讀區進行反轉錄酶-聚合酶鏈反應,發現在白點症病毒潛伏感染時期,草蝦體內確實有白點症病毒基因wssv194、wssv313與wssv369轉錄表現,其時序表現量呈現漸增之現象。而蛋白質序列預測分析顯示,此三個白點症病毒基因產物包含多種基因調節蛋白質之弁鄔夆炾魽A推測其可能參與病毒於潛伏時期的基因轉錄調控。For recent years, the detection of white spot syndrome virus (WSSV) of general cultured black tiger shrimps in Taiwan reveals that as high as 90% shrimps are WSSV carriers. Latent viral infection generates a long-term relationship between virus and its host. Under the latent state, few viral genes are expressed, which establishes the latency stage. The study examines the transcriptional expression of WSSV 532 predictive open reading frames and further identifies the WSSV latent-associated genes by the reverse transcriptase- DNA polymerase chain reaction. It was found that wssv194, wssv313 and wssv369 express in the WSSV carried Penaues monodom, and the expression level increases with the infection time. The protein sequence analysis reveals that some predictive functional motifs of gene regulatory protein exist in the WSSV latency-associated genes. These three latency-associated genes might possibly be involved in the transcriptional regulation of WSSV latent stage.前言…1
材料與方法…10
一、 人工感染試驗與草蝦檢體之收取…10
二、 檢體DNA之萃取與白點症病毒檢測…10
三、 檢體RNA之萃取…11
四、 反轉錄酶-聚合酶鏈反應…12
五、 快速增殖cDNA末端…13
1. 5’端(轉錄起始點)快速增殖cDNA末端…13
2. 3’端快速增殖cDNA末端…15
結果…16
蝦白點症病毒潛伏性感染相關基因之轉錄表現…16
蝦白點症病毒潛伏性感染相關基因之轉錄本分析…16
蝦白點症病毒潛伏性感染相關基因之胺基酸序列分析…18
討論…21
蝦白點症病毒潛伏性感染相關基因之轉錄表現…21
蝦白點症病毒潛伏性感染相關基因之轉錄調控探討…23
蝦白點症病毒潛伏性感染相關基因弁鈺敦Q…28
結論…32
參考文獻…33
圖表…48
附錄…60
附錄一、潛伏性相關基因於不同病毒分離株之ORF對照表…60
附錄二、本論文所使用之引子序列一覽表…6
From Erasmus to Maius
An-Ting Yi writes an extensive scholarly history of the famous Codex Vaticanus over the course of 350 years. Currently regarded as one of the most essential pillars to reconstruct the Greek New Testament text, the perceptions of this manuscript have changed profoundly in history, from an inferior witness to the most valuable foundation. This work uncovers how it became Codex Vaticanus par excellence in New Testament textual scholarship
Variations on the Author
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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