5,099 research outputs found
The Social Science of Living Alone w/ Dr. Jun Chu
No full textOn today's episode I speak with Dr. Jun Chu, Assistant Professor in the Department of Sociology, Anthropology, and Public Health at UMBC. Dr. Chu shares information about his ongoing research agenda into aging alone.Check out the following links for more information on UMBC, CS3, and our host:The UMBC Center for the Social Sciences ScholarshipThe University of Maryland, Baltimore CountyIan G. Anson, Ph.D.Retrieving the Social Sciences is a production of the UMBC Center for Social Science Scholarship. Our podcast host is Dr. Ian Anson, and our Acting director is Dr. Eric Stokan. Our production intern is Jean Kim. Our theme music was composed and recorded by D’Juan Moreland (UMBC '24). Special thanks to Amy Barnes and Myriam Ralston for production assistance. Make sure to follow us on Twitter, Facebook, Instagram, and YouTube, where you can find full video recordings of recent UMBC events.https://socialscience.umbc.edu/podcast/episode-60
Dolichyl-Phosphate Alpha-N-Acetyl Glucosaminyl Transferase (DPAGT1); A Target Enabling Package
No full textThe ER integral membrane enzyme dolichyl-phosphate alpha-N-acetyl glucosaminyl phosphotransferase (DPAGT1) catalyses the first step in the synthesis of the oligosaccharide-P-P-dolichol unit which provides the glycans structure for N-glycosylation of proteins. Mutations in DPAGT1 cause two muscle weakness conditions, limb-girdle congenital myasthenic syndrome (CMS) and congenital disorder of glycosylation type 1j (CDG1j). DPAGT1 overexpression has also been implicated in oral cancer. We have produced and solved structures of this integral membrane enzyme, DPAGT1 with the V264G mutation found in a patient with CMS, and complexes with a 50 nM inhibitor, tunicamycin. We have developed enzymatic activity and thermostability assays which have allowed us to assess the activity and stability of DPAGT1 mutants and the effect of small molecules. There are > 20 DPAGT1 associated missense variants in patients with CMS and CDG1j. We have mapped these mutations to the structure, and we will used the assays described here to assess how the activity and stability of DPAGT1 is affected by these missense variants
Investigating the interactions of cell surface receptors in brain development
No full textThe development of the central nervous system is a complex process which requires the coordination of neuronal guidance molecules and is poorly understood. Incorrect wiring of the brain network leads to neurological disorders such as autism, ADHD and schizophrenia. Among the guidance molecules, the Type-II dimeric receptor Teneurin and the adhesion GPCR Latrophilin have emerged as ancient interaction partners essential for synapse formation in vertebrates. Recently, the two- protein interaction has found to also incorporate the Fibronectin Leucine Rich Transmembrane (FLRT) receptor, an important adhesion receptor for brain and vascular development. The three- protein interaction is important for mammalian brain development: together they control the radial migration of neurons in the cortex using a mechanism of cell repulsion, whilst in the hippocampus they guide synaptic wiring using a mechanism of cell adhesion. These proteins display the ability to function in a context-dependent manner, however the regulatory mechanisms that result in their different signalling outcomes is not yet known. This thesis shows that the Latrophilin lectin domain binds to the tyrosine-aspartate-rich barrel domain of Teneurin at a highly conserved site, and the structure shows a configuration that is compatible with FLRT-binding. The existence of the ternary complex is validated by showing that Teneurin, Latrophilin and FLRT can be isolated as a complex from cells, and the interaction is supported by real-time interaction analyses on live cells. Furthermore, other previously unrevealed interactions involving different combinations of these proteins are explored. Collectively, this thesis starts to unravel the interactions underlying how these receptors orchestrate different aspects of brain development and provides the foundation for building their intricate interactome
The Localization Hypothesis and Machines
No full textIn a recent article in 'Artificial Life', Chu and Ho suggested that Rosen's central result about the simulability of living systems might be flawed. This argument was later declared ''null and void'' by Louie. In this article the validity of Louie's objections are examined
The Most Arrogant Man in France: Gustave Courbet and the Nineteenth-Century Media Culture
No full textBook Review of The Most Arrogant Man in France: Gustave Courbet and the Nineteenth-Century Media Culture / Petra ten-Doesschate Chu.--ISBN 978-0-691-12679-1. Reviewed by Amy S. Schwarz
Scientist at Work: Amy Chu on Green Chemistry, National Support for Groundbreaking Research with SMC Students, and ‘Thinking Cyclically’
No full textPublic Engagement and the Civic Professional: What's Next?
No full textThe media can be accessed here: http://streaming.osu.edu/knowledgebank/outreach06/100806b.mp4Moderator: Amy Driscoll (Associate Senior Scholar, Carnegie Foundation for the Advancement of Teaching). Panelists: David Mathews (President, Charles F. Kettering Foundation; Former Secretary of Health, Education, and Welfare in the
Ford Administration; Former President,
University of Alabama), Roderick G. Chu (Chancellor Emeritus, Ohio Board of Regents). Abstract: This session will feature a discussion circle of prominent leaders in key sectors who have spent much of their careers integrating civic
responsibility into their professional lives, as well as the organizations they represent. Organizations and individuals are doing their work differently as they commit to sharing in the obligations of a broad and diversified community. Colleges and universities, businesses, nonprofit organizations, and governmental agencies are supporting their employees as they integrate their work with the communities they serve. The ethos of engagement is shaping the educational and work choices of many young people, too. When professionals take on civic work, how is democracy served? How are we changed? What is the impact on institutions, organizations, and
professional cultures and practices? What are the implications for higher education?Outreach Scholarship and Imagining AmericaAmy Driscoll (00:00:00-00:04:46) -- David Mathews (00:04:47-00:19:05) -- Roderick G. Chu (00:19:06-00:30:50) -- Panel Discussion (00:30:51-01:17:19). [Note: The last few seconds of the panel discussion were not recorded.
Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip
No full textThe ongoing global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to active research in its associated diagnostics and medical treatments. While quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most reliable method to detect viral genes of SARS-CoV-2, serological tests for specific antiviral antibodies are also important as they identify false negative qRT-PCR responses, track how effectively the patient's immune system is fighting the infection, and are potentially helpful for plasma transfusion therapies. In this work, based on the principle of localized surface plasmon resonance (LSPR), we develop an opto-microfluidic sensing platform with gold nanospikes, fabricated by electrodeposition, to detect the presence and amount of antibodies specific to the SARS-CoV-2 spike protein in 1 mu L of human plasma diluted in 1 mL of buffer solution, within similar to 30 min. The target antibody concentration can be correlated with the LSPR wavelength peak shift of gold nanospikes caused by the local refractive index change due to the antigen-antibody binding. This label-free microfluidic platform achieves a limit of detection of similar to 0.08 ng/mL (similar to 0.5 pM), falling under the clinical relevant concentration range. We demonstrate that our opto-microfluidic platform offers a promising point-of-care testing tool to complement standard serological assays and make SARS-CoV-2 quantitative diagnostics easier, cheaper, and faster
Mutual disambiguation of recognition errors in a multimodal navigational agent
No full textThesis (M.Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2003.Includes bibliographical references (p. 69-71).by Amy Chu.M.Eng
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