76,518 research outputs found
Burmese amber fossils bridge the gap in the Cretaceous record of polypod ferns
No full textpublisher: Elsevier articletitle: Burmese amber fossils bridge the gap in the Cretaceous record of polypod ferns journaltitle: Perspectives in Plant Ecology, Evolution and Systematics articlelink: http://dx.doi.org/10.1016/j.ppees.2016.01.003 content_type: article copyright: Copyright © 2016 Elsevier GmbH. All rights reserved.Copyright © 2016 Elsevier GmbH. All rights reserved. This document is the authors' final accepted version of the journal article. You are advised to consult the publisher's version if you wish to cite from it.NHM Repositor
Amber lexicon in Wiktor Kozłowski’s <i>Słownik leśny, bartny, bursztyniarski i orylski</i>
No full textThe aim of this article is to examine the processes of nomination and ways of enriching the stock of special lexis concerning amber on the basis of the names collected in Wiktor Kozłowski’s Słownik leśny, bartny, bursztyniarski i orylski (Dictionary of Forestry, Beekeeping, Amber and Oryls, published 1846-1847). The author recorded vocabulary of varying territorial (mainly from central Poland) and socio-lectal range. We can arrange the recorded names semantically into three overarching groups: I. names pertaining to amber itself; II. names of people professionally involved in amber mining; III. names related to amber mining.This is the professional lexis used by amber workers when extracting amber from the ground. In the collection we find mainly nomenclatural names of objects. It contains lexemes taken from the general language and from dialects, as well as – conventionally enriching the stock of special lexis – word-forming neologisms, neosemantisms and borrowings.Celem artykułu jest zbadanie procesów nominacji i sposobów wzbogacania zasobu leksyki specjalnej dotyczącej bursztynu na podstawie nazw zgromadzonych w Słowniku leśnym, bartnym, bursztyniarskimi orylskim (wyd. 1846-1847) Wiktora Kozłowskiego. Autor notował słownictwo o różnym zasięgu terytorialnym (głównie z Polski centralnej) i socjolektalnym. Utrwalone w słowniku nazwy możemy uporządkować pod względem semantycznym w trzy nadrzędne grupy: I. nazwy dotyczące samego bursztynu; II. nazwy osób zajmujących się zawodowo wydobyciem bursztynu; III. nazwy związane z wydobyciem bursztynu. Jest to leksyka profesjonalna, używana przez bursztynników przy wydobywaniu żywicy z ziemi. W zbiorze odnajdujemy przede wszystkim nazwy nomenklaturowe obiektów. Znajdują się w nim leksemy zaczerpnięte z języka ogólnego oraz z gwar, a także – konwencjonalnie wzbogacające zasób leksyki specjalnej – zapożyczenia, neologizmy słowotwórcze oraz neosemantyzmy
ozbayb/amber: AMBER v1.0 release for multiphoton fiber bundle imaging
No full textAMBER V1.0
<p><strong>Artefactual Multiphoton Bundle Effect Removal</strong></p>
Description
<p>This release is a collection of functions and an example of a main program that is intended for the use of removing an artifact caused by performing multiphoton imaging through a coherent imaging fiber bundle. Some of the code may need to be modified to be compatible with specific file types for the image stacks. Also required is a "Flat" image, which is a multiphoton image of a flat fluorescent sample through the fiber-bundle acquired under the same conditions as the actual image data stacks to be fixed. The individual image stacks may be time series or Z-stacks and may be a field-of-view smaller than the full surface of the fiber-bundle. A simple rigid registration code allows the centroid locations identified in the flat image to be registered onto the image data.</p>
List of functions:
<ul>
<li>centerPadCrop: Performs a simple central crop</li>
<li>filterValSeries: Temporal/axial filter on each core independently</li>
<li>getCentroidValues: Extracts the values of the cores at the identified centroids</li>
<li>getCorrectorValues: Acquires the correction factors for each core from the flat image</li>
<li>getFiberCentroids: Finds the centroid pixel coordinates for each fiber core</li>
<li>getParameter: Extract specific data from a *.txt file containing imaging metadata</li>
<li>gridFiberCores: Interpolates the core centroids into a uniform pixel grid</li>
<li>makeFiberImage: Uses the centroid locations and values to recreate the fiber images</li>
<li>rigidAlignFiber: Performs a rigid registration of the flat fiber centroids to the actual image field</li>
<li>writeFiberImages: Writes the processed image files to *.tif with metadata (using Bioformats toolbox)</li>
</ul>
<p>Author: Baris N. Ozbay, University of Colorado Denver, Department of Bioengineering</p>
pFAR plasmids: New Eukaryotic Expression Vectors for Gene Therapy, devoid of Antibiotic Resistance Markers
No full textEfficient production of eukaryotic expression vectors requires the selection of plasmid-containing bacteria. To avoid the risk of dissemination of antibiotic resistance markers, we developed a new system to produce a family of plasmids Free of Antibiotic Resistance genes, called pFARs. The strategy is based on the suppression of a chromosomal nonsense mutation by a plasmid-borne function. The amber mutation was introduced into the Escherichia coli thyA gene that encodes a thymidylate synthase required for dTMP synthesis, resulting in thymidine auxotrophy. In parallel, a small plasmid vector that carries an amber suppressor t-RNA gene was entirely synthesised. The introduction of pFAR plasmids into an optimised thyA mutant restored normal growth to the auxotrophic strain, and led to an efficient production of monomeric supercoiled plasmids, as required for clinical trials. Luciferase activities measured after intramuscular injection and electrotransfer of LUC-encoding pFAR vector were similar to those obtained with a commercial vector containing the same expression cassette. Interestingly, whereas luciferase activities decreased within three weeks after intradermal electrotransfer of conventional expression vectors, sustained levels were observed with the pFAR derivative. Thus, pFAR plasmids represent a novel family of biosafe eukaryotic expression vectors, suitable for gene therapy
The Amber Room and Art under Nazism
No full textDiplomsko delo z naslovom Jantarna soba in umetnost v času nacizma govori o monopolu nad umetnostjo, ki ga je v Nemčiji in njenih osvojenih ozemljih vzpostavila nacistična stranka pod vodstvom Adolfa Hitlerja. Sprva avtorica obnovi potek druge svetovne vojne in predstavi, kaj je nacizem in kdo so bili glavni akterji v nacistični Nemčiji. Pojasni nacistično ideologijo umetnosti in se osredotoči na dogajanje v svetu umetnosti med leti 1939 in 1945. Natančneje se osredotoči na vpliv nacizma na Jude. V delu je razložen nadzor nad umetninami in umetniki, ropanje ter uničevanje umetniških del in prepoved ustvarjanja in razstavljanja neželenih umetnin. Avtorica predstavi najpomembnejše slikarje, kiparje in arhitekte v tistem času ter pojasni, kako so takrat delovali muzeji in kaj je bilo v njih razstavljeno. V drugem delu raziskuje zgodovino Jantarne sobe pred, med in po vojni. Predstavi različne teorije o njenem izginotju in glavne raziskovalce, ki so poskušali ugotoviti, kaj se je zgodilo z Jantarno sobo. Na koncu predstavi povezavo Jantarne sobe z nacistično ideologijo umetnosti.The diploma work, The Amber Room and Art under Nazism talks about the monopoly of art established by the NSDAP under the leadership of Adolf Hitler in Germany and the conquered territories. At first, the author describes the course of the Second World War and presents what Nazism was and who were the main actors in Nazi Germany. She writes about the Nazi ideology of art and focuses on what happened in the art world between 1939 and 1945. The focus is specifically on the impact of Nazism on the Jews. The author explains the control of art and artists, the looting and destruction of works of art, and the prohibition of creating and exhibiting undesirable art. The most important painters, sculptors and architects of that time are presented, and it is explained how museums have worked and what was exhibited in them. In the second part, the author goes into the history of the Amber Room before, during and after the war. She presents the various theories about her disappearance and the main researchers who tried to find out what happened to the Amber Room. Finally, she presents its connection with the National Socialist ideology of art
In the footsteps of amber mining in the Pomeranian province
No full textW artykule przedstawiono na podstawie analizy archiwalnych materiałów kartograficznych oraz literatury polsko- i niemieckojęzycznej z okresu XVI–XXI wieku rozmieszczenie dawnych kopalni bursztynu w województwie pomorskim. Rejestr dawnych wyrobisk opracowano w oparciu o technologię GIS. W efekcie wyznaczono 32 nieznane do tej pory historyczne miejsca wydobywania bursztynu. Na podstawie badania źródeł kartograficznych: XIX i XX-wiecznych pruskich map topograficznych w skali 1:25 000 (Urmesstischblätter, Messtischblätter) i arkuszy mapy geologicznej Prowincji Prusy w skali 1:100 000 (Geologische Karte der Provinz Preußen) uszczegółowiono lokalizację 11 historycznych stanowisk górniczych. W wielu przypadkach z jednym miejscem skojarzonych było więcej kopalni bursztynu. W rezultacie badanie archiwalnych pruskich map topograficznych umożliwiło dokładną lokalizację 35 dawnych kopalni bursztynu. Dodatkowo XIX-wieczne pruskie mapy geologiczne w skali 1:100 000 wizualizują lokalizację 9 kopalni. Analiza materiałów historycznych i przeprowadzone w terenie rozpoznanie wskazuje na relikty dawnego górnictwa bursztynowego szczególnie cenne z punktu widzenia dziedzictwa kulturowego, mało znane, a przy tym dobrze zachowane w terenie. Kompleksy te po zagospodarowaniu mogą stanowić wybitnie cenne zasoby geoturystyczne województwa pomorskiego.Article is based on analysis of archival cartographic materials and Polish–German literature from the period between the 16th and 21th centuries. It presents the sites of old amber mines in the Pomerania Province. In total 32 new and unknown amber mines were found and the study of historical topographic maps made it possible to establish the very exact location of places mentioned in previous works. The analysis of historical materials conducted by the author points to locations of unknown, well-preserved, old extraction sites, which are valuable due to their cultural heritage. After some investment in the local infrastructure, the sites can make attractive spots in the region. The geosite of Bursztynowe Kule’ in Tricity Landscape Park is one of such places were well-preserved old amber mines are to be found. The final result of library research is the GIS ‘Digital map of former amber mines in the Pomerania Province’
The Benefits of Being Economics Professor A (and not Z)
No full textAlphabetic name ordering on multi-authored academic papers, which is the convention in the economics discipline and various other disciplines, is to the advantage of people whose last name initials are placed early in the alphabet. As it turns out, Professor A, who has been a first author more often than Professor Z, will have published more articles and experienced afaster growth rate over the course of her career as a result of reputation and visibility. Moreover, authors know that name ordering matters and indeed take ordering seriously: Several characteristics of an author group composition determine the decision to deviate from the default alphabetic name order to a significant extent.performance measurement, incentives, economists, name ordering
Low-threshold Support Services For Addictions : A Brochure of Addiction-Based Services in Helsinki
No full textThe aim of this product-based thesis was to produce a brochure in the English language for addiction-based services available in Helsinki. The purpose of the brochure is to promote social inclusion for people who comprehend English and need addiction-based support. Four types of addictions are covered in this thesis: alcohol, narcotics, gambling, and video gaming. Part of the objective was to receive low-threshold services working within this field.
The new product was created for KRAN rf, which is a non-governmental organisation working in addiction-based care. KRAN rf provide services for people in any stage of the recovery process using Swedish, Finnish, English, and Russian languages.
Data was collected by sending surveys to professionals working in the field of addiction support. The survey was constructed in way to find out the key information that would be published in the brochure. Examples would include, on how to join the service, general contact details, languages used and low-threshold services. Explanations about the services and sectors were also added to help the reader. Once the data was collected it was organised and added to the brochure. The brochure was created on Adobe Photoshop using Envato Elements database to assist in the creation stage.
The final product was a bi-fold brochure, although a z-fold brochure was the initial plan. Three of the four addiction-based services were covered in the final product. All services mentioned in the brochure had services suitable for an English speaker.
The evaluation was conducted on the feedback of the product given by KRAN rf. The planning and implementation stages were also evaluated for the thesis, as to provide a clear report of how the product’s development could be improved or changed
In Vivo Incorporation of Multiple Unnatural Amino Acids
No full textUnnatural amino acid (UAA) incorporation is an invaluable technique that is seeing increased use. THG73 is an amber suppressor tRNA used to incorporate > 100 residues at the UAG, amber stop codon, in Xenopus oocytes. We have found that yeast Phe frameshift suppressors (YFFS) can incorporate UAAs at the CGGG and GGGU quadruplet codons in vitro and in vivo, allowing simultaneous incorporation of three UAAs in the nicotinic acetylcholine receptor (nAChR). The YFFS are more “orthogonal” than the amber suppressor tRNA, THG73, but the frameshift suppressors incorporate UAAs less efficiently than THG73. A library of tRNAs derived from THG73 has produced an amber suppressor that is “orthogonal” and suppresses similarly to THG73. An analogous opal suppressor tRNA allows incorporation of UAAs at the UGA, opal stop codon. The use of the amber, opal, CGGG, and GGGU codons should allow for the simultaneous incorporation of four UAAs in vivo. Bioorthogonal labeling of UAAs is useful for the addition of large fluorophores. We incorporated p-AcPhe at α70 of the nAChR and labeled with biotin and Cy5.5 hydrazide. Biotin and Cy5.5 hydrazide consistently labeled three proteins on oocytes not expressing α70p-AcPhe and isn’t useful for site-specific labeling of ketone containing UAAs in oocytes. We explored the known subunit stoichiometry of the nAChR (2α:β:γ:δ) expressed in oocytes and detected each subunit with the HA tag by Western blot. The α-subunit is present in excess of the other subunits in a ratio of ≈ 3:1, which is expected to be 2:1. UAAs are being sold commercially for detection of protein-protein interactions in eukaryotic cells. The UAAs are heterogeneously incorporated and little is known about the effect on protein function and stability. We heterogeneously incorporated UAAs into the nAChR and detected changes in function by shifts in EC50. Many UAAs altered the function of the nAChR. Incorporation of photo-reactive UAAs allowed for detection of cross-linking by Western blot. Heterogeneously incorporated UAAs also altered the functional nAChR expression on the surface of oocytes. Site-specific and heterogeneous incorporation of multiple UAAs are useful techniques for novel experiments to explore protein function, FRET experiments, cross-linking, and protein expression
The probabilistic termination tool amber
No full textWe describe the Amber tool for proving and refuting the termination of a class of probabilistic while-programs with polynomial arithmetic, in a fully automated manner. Amber combines martingale theory with properties of asymptotic bounding functions and implements relaxed versions of existing probabilistic termination proof rules to prove/disprove (positive) almost sure termination of probabilistic loops. Amber supports programs parametrized by symbolic constants and drawing from common probability distributions. Our experimental comparisons give practical evidence of Amber outperforming existing state-of-the-art tools
- …
