1,721,017 research outputs found
Evaluation of inflammation-associated C-reactive protein,IL-1B and VTD levels in patients with hyperthyroidism
Full text linkHipertiroidi, metabolizma hızını kontrol eden tiroid hormonlarının vücutta normalden fazla üretildiği bir sağlık sorunudur. Bu durum, metabolizmanın hızlı çalışmasına yol açar. Kalp atış hızı, vücut ısısı ve sindirim sistemi gibi birçok vücut fonksiyonu hızlanır. Hipertiroidi, kadınlarda daha sık görülür ve tedavi edilmediğinde ciddi hayati tehlike oluşturabilir. Hipertiroidi, tiroit bezinin aşırı miktarda tiroit hormonu üretmesiyle ortaya çıkan bir durumdur. Bu çalışmada hipertroidi hastaları ile kontrol grubunda inflamasyonla ilişkili C-reaktif protein (CRP) düzeyleri, D vitamini ve IL1β düzeyleri karşılaştırılmıştır. 2022-2023 yıllarında Düzce Üniversitesi Tıp Fakültesi İç Hastalıkları polikliniğine başvuran hipertroidi hastalarından alınan serum numuneleri kullanılarak veriler toplanmıştır. Analiz sonuçlarına göre hipertroidi hastalarında CRP düzeyleri kontrol grubu hastalarına oranının %42,01, d vitamini düzeyinde ise %78,03 olduğu tespit edilmiştir. İnflamasyonla ilişkili testlerde hipertroidi hastaları ve kontrol grubu test sonuçlarında istatistiksel anlamda herhangi bir ilişki tespit edilmemiştir (p<0,01).Hyperthyroidism is a health condition in which thyroid hormones, which control the metabolic rate, are produced more than normal in the body. This leads to a fast metabolism. Many body functions such as heart rate, body temperature and the digestive system are accelerated. Hyperthyroidism is more common in women and can be seriously life-threatening if left untreated. Hyperthyroidism is a condition that occurs when the thyroid gland produces excessive amounts of thyroid hormone. In this study, inflammation-related C-reactive protein (CRP) levels, vitamin D and IL1β levels were compared in hypertroid patients and control group. Data were collected using serum samples obtained from hypertroid patients who applied to Düzce University Faculty of Medicine Internal Medicine outpatient clinic in 2022-2023. According to the results of the analysis, it was determined that the ratio of CRP levels in hypertroid patients to control group patients was 42.01% and 78.03% in vitamin d level. There was no statistical correlation between the test results of hypertroid patients and control group in inflammation-related tests (p<0.01)
Investigation of the effect of quercetin on cell death pathways in HCT-116 and HT-29 colon cancer cell line
Full text linkKanser çok basamaklı, uzun süreli bir süreçtir ve sağlıksız bir hücrenin kontrolsüz bölünüp büyümesinin ardından tümör üreten hücre ölümünün yokluğu ile karakterizedir. Kolorektal kanserler dünyada en sık görülen kanserlerdendir Literatüre kalın bağırsağın iç yüzeyindeki hücrelerin kontrolsüz bölünmesiyle karakterize, yaygın gastrointestinal malign tümör olarak geçmiştir. Ülkemizde oluşan kanser türleri arasında ilk beşte yer alır ve görülme sıklığı giderek artmaktadır. Kanser araştırmalarındaki önemli zorluklardan biri sağlıklı hücreler bozulmadan, kanser hücrelerinin etkili bir şekilde nasıl öldürüleceğidir ve bu noktada kanserli hücreleri tedavi etmek için ölüm yolaklarının bilinmesi kritik bir önem taşır. Son yıllarda keşfedilmiş olan ferroptoz, demir bağımlılığı ile ortaya çıkan yeni bir programlanmış hücre ölümü türü olarak bilinir ve kanser hücreleri büyümeyi sağlamak için normal hücrelere kıyasla daha fazla demir talebi sergilediğinden kanser hücrelerinde ferroptozun indüklenmesinin çoklu kemoterapötiklerin direncini tersine çevirdiği ve kanser direncini azalttığı doğrulanmıştır. Bu çalışmalar ferroptozun tömör oluşumunda, gelişmesinde önemli bir düzenleyici olarak rol oynadığını göstermiştir. Flavonoidlerden kuersetin kanser önleme, ilerlemesini durdurma ve geleneksel kanser tedavilerinin yan etkilerini azaltılmasında başarılı olduğu bilinen metabolittir. Kuersetinin kolon kanserini engelleyici etkilerinin hücre ölüm yolakları üzerine etkilerinin ortaya çıkarılabilmesi için yapılan bu çalışmada kuersetin molekülünün kolon kanseri hücre hatlarında kemoterapik ajan ile kombine kullanımlarına ve tek kullanımlarının hücre canlılığı üzerindeki etkilerinin belirlenerek hücre ölüm mekanizmalarından apoptoz ve ferroptoz üzerindeki potansiyel etkilerinin değerlendirilmesi amaçlandı. Çalışmamızda öncelikle kuersetin ve kemoterapik ajan sitotoksik konsantrasyonları 3-(4,5-dimetiltiazol-2-il)- 2,5-difenil tetrazolyum bromür (MTT) testi ile HT-29 ve HCT-116 hücrelerinde 14 belirlendi. Daha sonra sitotoksik kuersetin ve kemoterapik ajan ile 24 saat inkübe edilen HCT-116 ve HT-29 hücreleri Apoptotik Proteaz Aktive Edici Faktör-1 (APAF-1), İnsan Kaspaz-3 Proteini (CASP-3), Uzun Zincirli Yağ Asidi-KoA Ligaz (ACSL4) ve Glutatyon Peroksidaz 4 (GPx4) seviyeleri enzime bağlı immünosorbent testi yöntemiyle (ELİSA) belirlendi. Çalışmada kuersetin molekülünün her iki kolon kanseri hücresinde tek başına APAF-1 ve CASP-3 biyobelirteçlerini kontrol grubuna göre arttırarak apoptotik yolağı aktive ettiği belirlenmiştir. GPx4 ve ACSL4 biyobelirteçlerinde ise kombine uygulamanın ACSL4'ü arttırıp GPx4'ü azaltarak ferroptotik yolağı indükleyip kanserli hücrede sinerjik etki yaptığı belirlenmiştir. Yapılan bu çalışma sonucundaki veriler kolon kanserine karşı kombine veya diğer alternatif yaklaşımlarına temel veri sağlayıp kanser tedavisi için ferroptoz sinyal yolağının daha detaylı araştırılmasında etken maddenin kullanımına yönelik çalışmalara katkı sağlayabilir.Cancer is a multi-step and long-term process and is characterized by the uncontrolled division and growth of an unhealthy cell followed by the absence of tumor-producing cell death. Colorectal cancers are among the most common cancers in the world. It is one of the first five types of cancer in our country and its incidence is increasing. One of the major challenges in cancer research is how to kill cancer cells effectively before healthy cells are destroyed, and at this point, knowing the death pathways is critical to treating cancerous cells. Discovered in recent years, ferroptosis is known as a new type of programmed cell death induced by iron dependence, and it has been confirmed that induction of ferroptosis in cancer cells reverses the resistance of multiple chemotherapeutics and reduces cancer resistance, as cancer cells exhibit greater demand for iron than normal cells to promote growth. These studies have shown that ferroptosis plays an important role as an important regulator in tumor formation and development. Quercetin, one of the flavonoids, is a metabolite known to be successful in preventing cancer, stopping its progression, and reducing the side effects of conventional cancer treatments. In this study, which was conducted to reveal the effects of quercetin inhibitory effects on colon cancer, on cell death pathways, it was aimed to evaluate the potential effects of quercetin molecule on apoptosis and ferroptosis by determining the effects of quercetin molecule in combination with a chemotherapy agent in colon cancer cell lines and the effects of single use on cell viability. In our study, first of all, quercetin and chemotherapeutic agent cytotoxic concentrations were determined in HT- 29 and HCT-116 cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test. HCT-116 and HT-29 cells, which were then incubated for 24 hours with cytotoxic quercetin and chemotherapeutic agent, Apoptotic Protease Activating 16 Factor-1(APAF-1), Human Caspase-3 Protein (CASP-3), Long Chain Fatty Acid-CoA Ligase (ACSL4) and Glutathione Peroxidase 4 (GPx4) levels were determined by enzyme-linked immunosorbent assay method (ELISA). In the study, it was determined that the quercetin molecule alone activates the apoptotic pathway by increasing APAF-1 and CASP-3 biomarkers in both colon cancer cells compared to the control group. In GPx4 and ACSL4 biomarkers, it was determined that the combined application had a synergistic effect on cancer cells by increasing ACSL4 and decreasing GPx4, inducing the ferroptotic pathway. The data from this study can provide basic data for combined or other alternative approaches against colon cancer. Further investigation of the ferroptosis signaling pathway for cancer treatment may contribute to studies on the use of the active substance
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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