1,721,218 research outputs found
Parathyroid hormone-related peptide and parathyroid hormone-related peptide receptor in breast cancer mcf7 cells
No full textThe aim of this work was to clarify the nature and the affinity state of the PTHrP receptor in breast cancer MCF7 cells compared to the classical PTH/PTHrP receptor expressed in the SaOS-2 osteosarcoma cell line.Quantitative equilibrium ligand binding assays and competitive binding assays using 125I-PTHrP-1-34Tyrosine demonstrated that PTHrP bound with high affinity in both cell lines, MCF7 and SaOS-2. However, KD values were not the same in both cell types. Ligand-receptor labelling followed by SDS-PAGE analysis revealed the presence of a single 85,000 Dalton band in both cell lines, which was thought to be the PTH/PTHrP receptor, since ligand binding was specifically inhibited by excess of unlabelled ligand.The substitution at position 23 of PTHrP caused an increase in the KD values for equilibrium ligand binding from 2.0 nM to 2.75 nM in MCF7 cells and from 5.0 nM to 5.4 nM in SaOS-2 cells. However, when both amino acids at positions 5 and 23 (His5 and Phe23) in the PTHrP molecule were substituted for those in PTH (Ile5 and Trp23), the affinity of the PTHrP receptor decreased to 4.1nM in MCF7 cells similar to that for PTH (4.25 nM) in MCF7 cells.The PTHrP receptor expression in MCF7 cells was increased following exposure to EGF or E2, whereas 1,25 DHCC inhibited the receptor expression in MCF7 cells, while DEX had no effect. In contrast, 1,25 DHCC and E2 treatment increased PTH/PTHrP receptor expression in SaOS-2 cells whereas EGF did not, while DEX inhibited the PTH/PTHrP receptor expression in SaOS-2 cells. This suggests that receptor expression is differently regulated in the two cell lines.The results suggest that the PTHrP receptor in MCF7 cells has many characteristics which distinguish from the receptor in SaOS-2 cells. The control of both PTHrP receptor expression and peptide production also differs between these two cell lines. These data show for the first time that there are higher affinity receptors for PTHrP in MCF7 breast cancer cells.</p
Correction to:PTH1 receptor agonists for fracture risk: a systematic review and network meta-analysis (Osteoporosis International, (2025), 10.1007/s00198-025-07440-1)
Get PDFThe original online version of this article was revised: In this article, the author Olivier Bruyère's name was missing; the order in which the authors appeared in the author list was incorrectly given as: Charlotte Beaudart 1,2 · Nicola Veronese 1,3 · Jonathan Douxfils 4,5,6 · Jotheeswaran Amuthavalli Thiyagarajan 7 · Francesco Bolzetta 8 · Paolo Albanese 8 · Gianpaolo Voltan 8 · Majed Alokail 9 · Nicholas C. Harvey 1,10 · Nicholas R. Fuggle 1,10 · René Rizzoli 1,11 · Jean‑Yves Reginster 1,9 where it should have been: Charlotte Beaudart 1,2, Nicola Veronese 1,3, Jonathan Douxfils 4,5,6, Jotheeswaran Amuthavalli Thiyagarajan 7, Francesco Bolzetta 8, Paolo Albanese 8, Gianpaolo Voltan 8, Majed Alokail 9, Nicholas C. Harvey 1,10, Nicholas R. Fuggle 1,10, Olivier Bruyère 1,11, René Rizzoli 1,12, Jean-Yves Reginster 1,9 In this article, the affiliation “Research Unit in Public Health, Epidemiology and Health Economics, University of Liege, Liege, Belgium” for Olivier Bruyère was missing. The original article has been corrected.</p
Effects of probiotics in patients with diabetes mellitus type 2 : study protocol for a randomized, double-blind, placebo-controlled trial
Get PDFBackground:
Low grade chronic inflammation is observed in patients with type 2 diabetes mellitus (T2DM). Endotoxin derived from gut bacteria may act as a potent inflammatory stimulant. Probiotics, which are believed to contain health promoting live microorganisms, may influence circulating endotoxin levels. Ingestion of live probiotic cultures may alter gut microbiota in a beneficial manner to reduce inflammation; no information is available whether or not they do so in patients with T2DM. Therefore, the aim of this study is to characterize the beneficial effects of probiotics on circulating endotoxin levels and other biomarkers related to systemic low-grade inflammation in patients with T2DM.
Methods:
One hundred and twenty consenting adult Saudi T2DM patients (naïve or newly diagnosed and without co-morbidities) will be enrolled in this clinical trial and randomized to receive daily placebo or probiotics (Ecologic®Barrier) for 26 weeks in a double-blind manner. Inflammatory and metabolic markers will be measured and fecal samples analyzed. Measurements/samples will be obtained at baseline and after 4, 8, 12/13 and 26 weeks of treatment.
Discussion:
It is expected that the probiotic product will induce beneficial changes in gut microbiota, reduce the systemic inflammatory state through altering systemic endotoxin levels and, as such, reduce the systemic inflammatory response observed in T2DM subjects.
Trial registration: ClinicalTrials.gov Identifier: NCT0176551
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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