1,720,968 research outputs found
Çoklu ilaç dirençlı tüberküloza karşı antıtüberküloz ilaçları ile birlikte nar suyunun kombınasyonu ve bu karışımın direnç ve efflux pompa sistem genlerine etkisinin incelenmesı.
No full textTEZ11209Tez (Doktora) -- Çukurova Üniversitesi, Adana, 2018.Kaynakça (s. 135-210) var.xxii, 211 s. : res. (bzs. rnk.), tablo ; 29 cm.Bu çalışmada amaç, taze nar suyunun (TNS=FPJ) tek başına ve şu ana kadar tedavide kullanılanılmakta olan antitüberküloz ilaçlarından Rifampin (R) ve Isoniazid (INH)’ ile kombinasyonlarının MDR-TB klinik izolatları üzerine antibakteriyel aktivitesini araştırmaktır .Dört adet taze nar suyu konsantrasyonu ile ilaca dirençli izolatlara karşı sırasıyla; (FPJ) (% 5,% 10,% 15 ve% 20), R ve INH ile kombinasyon halinde (1.0 ug / ml) ve (0.1ug / ml) doz aralığında değerlendirilmiştir. Ayrıca, çalışmamızda yüksek performanslı sıvı kromatografisi (HPLC) kullanılarak FPJ'nin tek tek fenolik bileşikleri araştırılarak; FPJ'de toplam polifenoller (TP), toplam flavonoid (TF), toplam antosiyanin içeriği (TAC) ve antioksidan kapasite de değerlendirilmiştir. Çalışmamızda, INH ve R direnci ile ilişkili dirençli genler ile altı efflux pompa genlerinin MDR-TB klinik izolatlarındaki ilaç direnci seviyelerine katkısı da değerlendirilmiştir. MDR-TB izolatlarında KatG'nin ekspresyon seviyesi 24/27 (%88.88) 'de azalırken; MDR-TB izolatlarında sırasıyla 8 /27 (% 29.62) ve 4/27 (% 14.81), inhA, oksiR-ahpC ve rpoB genleri 24 /27'de (% 88.88) yüksek düzeyde eksprese edilmiş veya artmıştır. Ayrıca, efflux pompa genleri drrA, drrB, efpA, Rv2459, Rv1634 ve Rv1250, INH/R ile FPJ kombinasyonu etkisinde, yüksek düzeyde eksprese edilerek MDR-TB izolatlarının genel direncine katkı gösterdiği görülmüştür.The aim of present study was to investigate the effect of fresh pomegranate juice (FPJ) on the antibacterial activity of anti-tuberculosis drugs (Rifampin (R) and Isoniazid (INH)) against MDR-TB clinical isolates. Four concentrations of fresh pomegranate juice (FPJ) (5%, 10%, 15%, and 20%) were evaluated in combination with R and INH at a dose range of (1.0 ?g/ml), and (0.1?g/ml), respectively. Moreover, this study investigated individual phenolic compounds of FPJ by using highperformance liquid chromatography (HPLC). The total polyphenols (TP), total flavonoid (TF), total anthocyanins content (TAC), and the antioxidant capacity were also assessed in FPJ. Additionally,we evaluated the contribution of resistant genes associated with INH and R resistance plus six putative drug efflux pump genes to the drug resistance levels in MDR-TB clinical isolates. While the expression level of katG was down-regulated in 24/27 (88.88%) MDR-TB isolates, inhA, oxyR-ahpC, and rpoB appeared to be either overexpressed or up-regulated in 8/ 27 (29.62%), 4/27 (14.81 %), and 24/ 27 (88.88%), respectively in MDR-TB isolates. Moreover, the efflux pump genes drrA, drrB, efpA, Rv2459, Rv1634, and Rv1250 were overexpressed under INH/RIF plus FPJ stressBu çalışma Ç.Ü. Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir. Proje No: FDK-2017-8223
Identifying the developmental stages and optimizing the sample preparation for anatomical study of Orthosiphon stamineus
No full textThis study focuses on the characterization of morphological and anatomical traits of Orthosiphon stamineus which belongs to the Lamiaceae family. Orthosiphon stamineus, better known as "Misai Kucing" or "cats whiskers" by the locals, contained active phenolics compounds such as flavanoids. Despite its wide usage as a medicinal plant, information regarding Orthosiphon stamineus specific developmental stages is relatively scarce. Furthermore, to date, no anatomical data of this plant is available. Therefore, this study aims to systematically identify the developmental stages and its anatomy which may provide more insight to its medical application. The result showed some distinct morphological and anatomical characteristics. In the morphological study, it was observed that Orthosiphon stamineus is a herbal shrub with well-developed creeping rootstock. The leaves are simple, green, and arranged in opposite pairs. The stem is approximately 54 cm in height at the oldest stage (62- 64 days). In order to proceed with the anatomical study of Orthosiphon stamineus, the cross sections of different developmental stages of leaves and stems were examined. Several critical steps prior to viewing the prepared slides, which include dehydration, sectioning and staining, were optimized accordingly
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Cladosporium Cladosporioides from the Perspectives of Medical and Biotechnological Approaches
Get PDFFungi are important natural product sources that have enormous potential for the production of novel compounds for use in pharmacology, agricultural applications and industry. Compared with other natural sources such as plants, fungi are highly diverse but understudied. However, research on Cladosporium cladosporioides revealed the existence of bioactive products such as p-methylbenzoic acid, ergosterol peroxide (EP) and calphostin C as well as enzymes including pectin methylesterase (PME), polygalacturonase (PG) and chlorpyrifos hydrolase. p-Methylbenzoic acid has ability to synthesise 1,5-benzodiazepine and its derivatives, polyethylene terephthalate and eicosapentaenoic acid. EP has anticancer, antiangiogenic, antibacterial, anti-oxidative and immunosuppressive properties. Calphostin C inhibits protein kinase C (PKC) by inactivating both PKC-epsilon and PKC-alpha. In addition, calphostin C stimulates apoptosis in WEHI-231 cells and vascular smooth muscle cells. Based on the stimulation of endoplasmic reticulum stress in some types of cancer, calphostin C has also been evaluated as a potential photodynamic therapeutic agent. Methylesterase (PME) and PG have garnered attention because of their usage in the food processing industry and significant physiological function in plants. Chlorpyrifos, a human, animal and plant toxin, can be degraded and eliminated by chlorpyrifos hydrolase
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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