1,720,954 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Tuning Post-Translational Modifications in Mammalian Cells: Exploring Media Optimization and Gene Editing Strategies
No full textThe production of biologics has revolutionized the treatment of numerous diseases, offering targeted high-efficacy therapeutics for conditions ranging from cancer to autoimmune disorders. These biologics are typically produced using mammalian cells, requiring carefully optimized manufacturing processes to ensure high yields and product quality attributes that meet regulatory standards.
However, the manufacturing of these complex therapeutics is challenged by the need for finely tuned cell culture conditions to ensure optimal product yield and quality. Small changes in cell culture conditions can significantly impact cell metabolism and protein quality attributes, potentially rendering the final product unsuitable for therapeutic use.
This thesis looks to address the challenge of modulating product quality attributes by employing gene editing techniques to develop cell lines tailored for producing recombinant proteins with desirable quality attributes and refining cell culture media formulations to control the availability of key components in media.
First, as a model case, we demonstrate the impact of product quality on therapeutic efficacy using butyrylcholinesterase (HuBChE), a human enzyme of significant value as a medical countermeasure against organophosphate poisoning. Given the scarcity of HuBChE in human plasma, recombinant production in mammalian cells is necessary to meet therapeutic demands. Through gene editing, we established a mammalian production platform that generates recombinant HuBChE with product quality attributes closely resembling the native human enzyme.
Next, we explored the regulation of metal availability in cell culture media, focusing on copper as a critical trace element that affects cell growth and protein PTMs. Using copper-specific chelating agents, we developed copper-buffered systems to maintain stable and bioavailable copper levels throughout the culture process. This controlled environment mitigates the adverse effects of copper fluctuations, supporting consistent cell performance and ensuring predictable quality attributes in the produced therapeutics.
Next, we seek to elucidate solubility interactions between different amino acids in aqueous solutions. By studying combinations of two amino acids in water, we identified negative, neutral, and positive interactions between amino acids. These experiments were also extended to see how amino acid solubilities were affected as a function of salt concentrations in solution as well, to ultimately guide more efficient cell growth media design
Tuning Post-Translational Modifications in Mammalian Cells: Exploring Media Optimization and Gene Editing Strategies
No full textThe production of biologics has revolutionized the treatment of numerous diseases, offering targeted high-efficacy therapeutics for conditions ranging from cancer to autoimmune disorders. These biologics are typically produced using mammalian cells, requiring carefully optimized manufacturing processes to ensure high yields and product quality attributes that meet regulatory standards.
However, the manufacturing of these complex therapeutics is challenged by the need for finely tuned cell culture conditions to ensure optimal product yield and quality. Small changes in cell culture conditions can significantly impact cell metabolism and protein quality attributes, potentially rendering the final product unsuitable for therapeutic use.
This thesis looks to address the challenge of modulating product quality attributes by employing gene editing techniques to develop cell lines tailored for producing recombinant proteins with desirable quality attributes and refining cell culture media formulations to control the availability of key components in media.
First, as a model case, we demonstrate the impact of product quality on therapeutic efficacy using butyrylcholinesterase (HuBChE), a human enzyme of significant value as a medical countermeasure against organophosphate poisoning. Given the scarcity of HuBChE in human plasma, recombinant production in mammalian cells is necessary to meet therapeutic demands. Through gene editing, we established a mammalian production platform that generates recombinant HuBChE with product quality attributes closely resembling the native human enzyme.
Next, we explored the regulation of metal availability in cell culture media, focusing on copper as a critical trace element that affects cell growth and protein PTMs. Using copper-specific chelating agents, we developed copper-buffered systems to maintain stable and bioavailable copper levels throughout the culture process. This controlled environment mitigates the adverse effects of copper fluctuations, supporting consistent cell performance and ensuring predictable quality attributes in the produced therapeutics.
Next, we seek to elucidate solubility interactions between different amino acids in aqueous solutions. By studying combinations of two amino acids in water, we identified negative, neutral, and positive interactions between amino acids. These experiments were also extended to see how amino acid solubilities were affected as a function of salt concentrations in solution as well, to ultimately guide more efficient cell growth media design
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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