129 research outputs found
A novel microtubule de-stabilizing complementarity-determining region C36L1 peptide displays antitumor activity against melanoma in vitro and in vivo
No full textIntercalation of polypyrrole into graphite oxide
No full textIn this paper we report on the insertion of polypyrrole into layered graphite oxide. This was achieved by using the exfoliating and re-stacking properties of the host. The resulting intercalated product was characterized by powder X-ray diffraction, FT-IR spectroscopy, thermogravimetric analysis and scanning electron microscopy. (c) 2006 Elsevier B.V. All rights reserved.PT: J; CR: *ACS, 1996, ACS SYM SER, P622 BISSESSUR R, 2001, CHEM COMMUN 0907, P1598 BISSESSUR R, 2003, J MATER CHEM, V13, P44 BISSESSUR R, 2004, J MATER SCI, V39, P119 CASSAGNEAU T, 1998, ADV MATER, V10, P877 CASSAGNEAU T, 2000, LANGMUIR, V16, P7318 DING RF, 2003, POLYM DEGRAD STABIL, V81, P473 HUMMERS WS, 1958, J AM CHEM SOC, V80, P1339 KANATZIDIS MG, 1993, CHEM MATER, V5, P595 KOVTYUKHOVA NI, 1999, CHEM MATER, V11, P771 LIU ZH, 2002, LANGMUIR, V18, P4926 MATSUO Y, 1997, CARBON, V35, P113 MATSUO Y, 1998, CHEM MATER, V10, P2266 MATSUO Y, 2002, J MATER CHEM, V12, P1592 NAKAJIMA T, 1988, CARBON, V26, P357 WANG L, 1995, CHEM MATER, V7, P1753 WU CG, 1989, POLYM MATER SCI ENG, V61, P969 XIAO M, 2002, POLYMER, V43, P2245 XIAO P, 2000, CARBON, V38, P623; NR: 19; TC: 0; J9: SYNTHET METAL; PG: 5; GA: 093UXSource type: Electronic(1
The Ig VH complementarity-determining region 3-containing Rb9 peptide, inhibits melanoma cells migration and invasion by interactions with Hsp90 and an adhesion G-protein coupled receptor
No full textThe present work aims at investigating the mechanism of action of the Rb9 peptide, which contains the VHCDR 3 sequence of anti-sodium-dependent phosphate transport protein 2B (NaPi2B) monoclonal antibody RebMab200 and displayed antitumor properties. Short peptides corresponding to the hypervariable complementarity-determining regions (CDRs) of immunoglobulins have been associated with antimicrobial, antiviral, immunomodulatory and antitumor activities regardless of the specificity of the antibody. We have shown that the CDR derived peptide Rb9 induced substrate hyperadherence, inhibition of cell migration and matrix invasion in melanoma and other tumor cell lines. Rb9 also inhibited metastasis of murine melanoma in a syngeneic mouse model. We found that Rb9 binds to and interferes with Hsp90 chaperone activity causing attenuation of FAK-Src signaling and downregulation of active Rac1 in B16F10-Nex2 melanoma cells. The peptide also bound to an adhesion G-protein coupled receptor, triggering a concentration-dependent synthesis of cAMP and activation of PKA and VASP signaling as well as IP-3 dependent Ca2+ release. Hsp90 is highly expressed on the cell surface of melanoma cells, and synthetic agents that target Hsp90 are promising cancer therapeutic drugs. Based on their remarkable antitumor effects, the CDR-H3-derived peptides from RebMab200, and particularly the highly soluble and stable Rb9, are novel candidates to be further studied as potential antitumor drugs, selectively acting on cancer cell motility and invasion
Anti-tumor activities of peptides corresponding to conserved complementary determining regions from different immunoglobulins
No full textShort synthetic peptides corresponding to sequences of complementarity-determining regions (CDRs) from different immunoglobulin families have been shown to induce antimicrobial, antiviral and antitumor activities regardless of the specificity of the original monoclonal antibody (mAb). Presently, we studied the in vitro and in vivo antitumor activity of synthetic peptides derived from conserved CDR sequences of different immunoglobulins against human tumor cell lines and murine B16F10-Nex2 melanoma aiming at the discovery of candidate molecules for cancer therapy. Four light-and heavy-chain CDR peptide sequences from different antibodies (C36-L1, HA9-H2, 1-H2 and Mg16-H2) showed cytotoxic activity against murine melanoma and a panel of human tumor cell lineages in vitro. Importantly, theyalso exerted anti-metastatic activity using a syngeneic melanoma model in mice. Other peptides (D07-H3, MN20v1, MS2-H3) were also protective against metastatic melanoma, without showing significant cytotoxicity against tumor cells in vitro. in this case, we suggest that these peptides may act as immune adjuvants in vivo. As observed, peptides induced nitric oxide production in bone-marrow macrophages showing that innate immune cells can also be modulated by these CDR peptides. the present screening supports the search in immunoglobulins of rather frequent CDR sequences that are endowed with specific antitumor properties and may be candidates to be developed as anti-cancer drugs. (C) 2014 Elsevier Inc. All rights reserved.Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Expt Oncol Unit UNONEX, São Paulo, SP, BrazilUniv Parma, Dept Biomed Biotechnol & Translat Sci, Microbiol & Virol Unit, I-43121 Parma, ItalyRecepta Biopharma, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Expt Oncol Unit UNONEX, São Paulo, SP, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 2010/51423-
A protective effect of dietary calcium against acute waterborne cadmium uptake in rainbow trout
No full textThe present study examined the interactions between elevated dietary calcium (as ionic Ca2+ in the form of CaCl2 x 2H2O) and acute waterborne Cd exposure (50 microg/l as CdNO3 for 3 h) on whole body uptake and internal distribution of newly accumulated Cd, Ca2+, and Na+ in juvenile rainbow trout (Oncorhynchus mykiss). Fish were fed with three diets 20 (control), 30 and 60 mg Ca2+/g food: for 7 days before fluxes were measured with radiotracers over a 3h period. The two elevated Ca2+ diets reduced the whole body uptake of both Ca2+ and Cd by >50% and similarly reduced the internalization of both newly accumulated metals in most tissues, effects which reflect the shared branchial uptake route for Ca2+ and Cd. As the Ca2+ concentrations of the fluid phases of the stomach and intestinal contents were greatly elevated by the experimental diets, increased gastrointestinal Ca2+ uptake likely caused the down-regulation of the branchial Ca2+ (and Cd) uptake pathway. Waterborne Na+ uptake and internal distribution were not affected. While plasma Ca2+ surged after the first two feedings of the 60 mg Ca2+/g diet, internal homeostasis was quickly restored. Total Ca2+, Na+, and Cl- levels in tissues were not affected by diets. While dietary Ca2+ protected against waterborne Cd uptake, it did not protect against the relative inhibition of waterborne Ca2+ uptake caused by waterborne Cd. Acute exposure to 50 microg/l Cd reduced the uptake and internalization of newly accumulated Ca2+ (but not Na+) by 70% or more, regardless of diet. Since elevated dietary Ca2+ reduces waterborne Cd uptake, fish eating a Ca(2+)-rich invertebrate diet may be more protected against waterborne Cd toxicity in a field situation.LR: 20061115; PUBM: Print; JID: 8500246; 0 (Calcium, Dietary); 0 (Water Pollutants, Chemical); 7440-23-5 (Sodium); 7440-43-9 (Cadmium); 2003/05/23 [received]; 2003/11/28 [revised]; 2003/12/01 [accepted]; ppublishSource type: Electronic(1
Correction to: Trans-ancestral dissection of urate- and gout-associated major loci SLC2A9 and ABCG2 reveals primate-specific regulatory effects
No full text© 2020, The Author(s), under exclusive licence to The Japan Society of Human Genetics. The original version of this article unfortunately contained a mistake. One of members of Eurogout consortium wasn’t included. The corrected version is given below. Eurogout consortium A. Abhishek, M. Andres, T. Crisan, N. Dalbeth, M. Doherty, L. Jacobsson, M. Janssen, T.L. Jansen, L.A. Joosten, M. Kapetanovic, F. Lioté, H. Matsuo, G. McCarthy, T. Merriman, F. Perez-Ruiz, P.L. Riches, P. Richette, P.C. Robinson, E. Roddy, B. Stiburkova, A. So, L.K. Stamp, A.K. Tausche, R. Torres-Jiminez, T. Uhlig
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Performing the Author-Translator Across Shores: Japanese Refractions of World and Latin American Literature in the 20th Century
No full textThis dissertation intervenes in current scholarly debates surrounding East-West literary exchanges and the role that Western literary and academic cultural networks play in the mediation of transcultural literary relationships between the Global South and non-Western cultural spaces. It does so by analyzing the translations and intertextualizations of Japanese literature in Latin American literature during the 20th century, and vice versa.
The critical argument of my project is centered on the figure that I have termed the “author-translator,” writers whose work in translation occurs not in parallel to professional or academic careers, but in direct relation with their endeavors as creators of works of literature. Author-translators approach the task of translation with a different set of considerations, expectations, and goals than those of a different ilk. My contention is that by treating such translators as a distinct category, and by then using the Japanese and Latin American examples of this phenomenon as a case study, we can shed light on the larger processes of literary circulation and world literature canon-formation in contemporary global literature.
The Introduction provides a substantial account of how Latin American literature was read in Japan during the 20th century, while also establishing the theoretical framework of the dissertation in regard to translation studies, postcolonialism, and world literature studies, the three vectors on whose intersection this dissertation lies. Chapter 1 analyzes Mexican poet Octavio Paz’s early stays in India and Japan, and his translation of Matsuo Bashō’s The Narrow Road to the North (1st ed. 1952, 2nd ed.1970). Chapter 2 discusses how Borges has been read in Japan since the 1950s up to the present day, but also pays special attention to Borges’s own transculturation of Japanese literary themes in the form of his tanka, haiku, and short fiction, and how these have been translated into Japanese. Chapter 3 analyzes Terayama Shūji’s 1980s adaptations—first for the stage and later for film—of García Márquez’s novel One Hundred Years of Solitude. Chapter 4 proceeds to the 1990s, tracing Murakami Haruki’s career as an influential translator of American literature in Japan before analyzing his 1992 travel narrative to Mexico, included in his 1998 travel book Distant Frontier, Close Frontier
Persistent contribution of unbound quasiparticles to the pair correlation in the continuum Skyrme-Hartree-Fock-Bogoliubov approach
No full textThe neutron pair correlation in nuclei near the neutron drip-line is investigated using the self-consistent continuum Skyrme-Hartree-Fock-Bogoliubov theory formulated with the coordinate-space Green's function technique. Numerical analysis is performed for even-even N=86 isotones in the Mo-Sn region, where the 3p3/2 and 3p1/2 orbits lying near the Fermi energy are either weakly bound or unbound. The quasiparticle states originating from the l=1 orbits form resonances with large widths, which are due to the low barrier height and the strong continuum coupling caused by the pair potential. Analyzing in detail the pairing properties and roles of the quasiparticle resonances, we found that the l=1 broad quasiparticle resonances persist to feel the pair potential and contribute to the pair correlation even when their widths are comparable with the resonance energy. © 2011 American Physical Society.Articl
How Historical Context Matters for Fourth and Fifth Generation Japanese Americans
No full textJapanese Americans have a longstanding history in the U.S.-- comprising of more than five consecutive generations. Yet generational research on this ethnic group is understudied (Meredith, Wenger, Liu, Harada, & Kahn, 2000; Pang, 2007). By connecting the historical experiences of previous generations of Japanese Americans to the present, findings on how history has impacted this population can be applied in other ethnic multi-generational groups in the United States.
An Ethnic Identity & Generational Status Model was developed by the author that was influenced by Jean Phinney (1990), Handlin (1951), Mannheim (1927), and Matsuo (1992) to support the varied roles that contribute to ethnic identity formation: history, family, education, and society. The purpose of this paper is to highlight the historical influences of fourth and fifth generation Japanese American identity.
40 participants who identify as fourth or fifth generation Japanese American on at least one side of their family through purposive snowball sampling were interviewed using an open ended questionnaire. This study will examine the historical responses from the interview protocol. Therefore, the research question in this paper asks, “How does history influence the ethnic identity of fourth and fifth generation Japanese American adults?
Isolamento e avaliação do potencial citotóxico de derivados fenólicos de Schinus terebinthifolius Raddi (Anacardiaceae)
No full textThe EtOH extract from leaves of S. terebinthifolius was subjected to partition between EtOH:H2O and hexane, CH2Cl2, and EtOAc. The phases obtained were evaluated in vitro against human tumoral cell lines and the EtOAc phase exhibited activity. Chromatographic procedures afforded gallic acid (1), methyl (2) and ethyl (3) gallates, trans-catechin (4), quercitrin (5), and afzelin (6), being the first occurrence of 1, 4 and 6 in S. terebinthifolius.In vitro cytotoxic evaluation of 1 - 6 indicated that gallic acid (1) displayed higher activity than ethyl gallate (3) against HL-60 and HeLa cells, while compounds 2, 4 - 6 were inactive
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