87,341 research outputs found
Additional data for "Selenium drives a transcriptional adaptive program to block ferroptosis and treat stroke" by Alim et al.
No full textPaper Title: Selenium drives a transcriptional adaptive program to block ferroptosis and treat strokeThese are additional data sets from the related article. 1) Scatter plots for all behavioral data from the paper. GPX4 overexpression scatter plot: (A-B) Forced expression of GPX4 in vivo improves outcome following ICH. Scatterplot of behavioral outcomes in spatial neglect task (n=16; scatter plot) and sensory neglect task (n=16; scatter plot) for up to 14 days post ICH in mice overexpressing GFP or GPX4 in striatum.Selenium treatment scatter plots: (A-B) Scatterplot of behavioral outcomes including spatial neglect task (n=16) and sensory neglect task (n=16) up to 7 days post ICH with or without ICV injection of Se (2.5µM). (C-D) Tat SelPep improves outcomes when given systemically 2h after ICH. Scatterplot of behavioral outcomes in sensory neglect task (n=16) and spatial neglect task (n=16) for up to 14 days post ICH. (E-F) Tat SelPep improves behavioral outcomes with a therapeutic window up to 6 hours. Scatterplot of behavioral outcomes in spatial neglect task ( n=16) and sensory neglect task (n=16) for up to 14 days post ICH in mice given 6 hour post injury IP injections and every day for 7 days of saline, Tat or Tat SelPep (12µg/g). 2) Blots have not been modified with the exceptions of the labels. The file names on the blots correspond to the blots in the figures from the paper.3) Molecular depletion and validation of exon 5-6 (A-C) and exon 4 (D-F) targeted siRNA of GPX4. Raw blot images are included.4) (A-D) Differential Interference Contrast (DIC) time-lapse video examples of primary cortical neurons treated with (A) vehicle control, (B) Erastin (5µM), (C) Hemin (80µM), and (D) Hemin+Se (1µM Se). Yellow time bar shows relative hours passed from time of cotreatment, red arrows indicate primary cortical neurons destined to die. Scale bar 100µm.5) (A-E) ICH modeling in vitro or in vivo shows TUNEL labeling, one feature of ferroptosis. (A-C) HT22 hippocampal neuroblast cells exposed to (A) 100µM Hemin followed by TUNEL labeling, (B)Vehicle control followed by TUNEL labeling, and (C) Hemin without TdT nucleotide treatment followed by TUNEL labeling. White boxes show magnified image and arrows point to TUNEL positive cells. Scale bar 50µM. (D) TUNEL staining (green) of representative brain section from a mouse 7 days after post-saline volume control injection. Inserts represent magnified images with ToPro (blue) and/or TUNEL staining (green). Scale bar 50µM. (E) TUNEL staining (green) of brain section from a mouse 7 days after collagenase injection into the striatum (lCH model). Insert shows ToPro (blue) and/or TUNEL positive cells (green). Scale bar 50µM6) All hubs from WGCNA data are shown based on RNA-seq conducted in the paper. 7) Caspase inhibitor Z-VAD-FMK or Se protect against ER stress
ALIM ieri e oggi
No full textALIM (Archive of the Italian Latinity of the Middle Ages) aims to provide free online access to all the Latin texts produced in Italy during the Middle Ages. For several centuries, in fact, Latin represented the only language in which, in addition to historical documentation, many of the major creations of thought, science, and literature of the Middle Ages were expressed. Even when national languages imposed themselves in written form, Latin never lost its role and prestige as transnational language. Born in the Nineties, the original purpose for ALIM project is to integrate the linguistic information which, for the previous centuries, is made available in the Dictionary of Medieval Italian Latinity, promoted by the U.A.N. (National Academic Union) under the guidance of F. Arnaldi and P. Smiraglia (Latinitatis Italicae Medii Aevi lexicon, Florence 2001). In addition to providing critically reliable texts for the use of philologists, historians of literature, historians of institutions, culture, and science of the Middle Ages, the ALIM project aims to produce useful material for a dictionary of European Medieval Latin. The ultimate goal of the latter project is to collect and unify the lexicons produced by all the European states for their own medieval Latin texts
Order parameter allows classification of planar graphs based on balanced fixed points in the Kuramoto model
No full textPhase balanced states are a highly underexplored class of solutions of the Kuramoto model and other coupled oscillator models on networks. So far, coupled oscillator research focused on phase synchronized solutions. Yet, global constraints on oscillators may forbid synchronized state, rendering phase balanced states as the relevant stable state. If, for example, oscillators are driving the contractions of a fluid filled volume, conservation of fluid volume constrains oscillators to balanced states as characterized by a vanishing Kuramoto order parameter. It has previously been shown that stable, balanced patterns in the Kuramoto model exist on circulant graphs. However, which noncirculant graphs first of all allow for balanced states and what characterizes the balanced states is unknown. Here, we derive rules of how to build noncirculant, planar graphs allowing for balanced states from the simple cycle graph by adding loops or edges to it. We thereby identify different classes of small planar networks allowing for balanced states. Investigating the balanced states’ characteristics, we find that the variance in basin stability scales linearly with the size of the graph for these networks. We introduce the balancing ratio as an order parameter based on the basin stability approach to classify balanced states on networks and evaluate it analytically for a subset of the network classes. Our results offer an analytical description of noncirculant graphs supporting stable, balanced states and may thereby help to understand the topological requirements on oscillator networks under global constraints
Epistolae
No full textEdizione critica a stampa a cura di F. Delle Donne
compilato da ALIM
Edizione in formato TEI XML, livello ALIM2_1, a cura di Jan Ctibor
HomePage del progetto: http://it.alim.unisi.it/il-progetto/
Documentazione: http://alim.unisi.it/documentazion
Epistolae
No full textEdizione critica a stampa a cura di F. Delle Donne
compilato da ALIM
Edizione in formato TEI XML, livello ALIM2_1, a cura di Jan Ctibor
HomePage del progetto: http://it.alim.unisi.it/il-progetto/
Documentazione: http://alim.unisi.it/documentazion
Innocentius IV papa per Iordanum de Terracina
No full textEdizione critica a stampa a cura di F. Delle Donne
compilato da ALIM
Edizione in formato TEI XML, livello ALIM2_1, a cura di Jan Ctibor
HomePage del progetto: http://it.alim.unisi.it/il-progetto/
Documentazione: http://alim.unisi.it/documentazion
Epistolae
No full textEdizione critica a stampa a cura di F. Delle Donne
compilato da ALIM
Edizione in formato TEI XML, livello ALIM2_1, a cura di Jan Ctibor
HomePage del progetto: http://it.alim.unisi.it/il-progetto/
Documentazione: http://alim.unisi.it/documentazion
Caroli II regis epistolae per Iohannem Capuanum
No full textEdizione critica a stampa a cura di F. Delle Donne
compilato da ALIM
Edizione in formato TEI XML, livello ALIM2_1, a cura di Jan Ctibor
HomePage del progetto: http://it.alim.unisi.it/il-progetto/
Documentazione: http://alim.unisi.it/documentazion
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
De uxore cerdonis
No full textEdizione critica a stampa a cura di F. Bertini
compilato da ALIM
Edizione in formato TEI XML, livello ALIM2_1, a cura di Jan Ctibor
HomePage del progetto: http://it.alim.unisi.it/il-progetto/
Documentazione: http://alim.unisi.it/documentazion
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