20 research outputs found
Sortase-Mediated Ligation of Purely Artificial Building Blocks
No full textSortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP–polymer, NP–NP, and polymer–polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction—the conserved peptide LPETG—and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated.Xiaolin Dai and Ulrich Glebe thank Walter-Georg Tillmann, Khosrow Rahimi, Sabrina Mallmann,
Joerg Fettke, Manfred Pinnow, Andreas Bohn, Steffi Grunst, Erik Wischerhoff, and René Kalbitz for help with
analytical measurements. Tayebeh Mirzaei Garakani acknowledges the Alexander von Humboldt-Stiftung for
financial support. Part of this work was performed at the Center for Chemical Polymer Technology (CPT) unit of
DWI, which was supported by the EU and the federal state of North Rhine–Westphalia (grant EFRE 30 00 883 02)
Das Rechenzentrum als Produktionsstätte für IT-Dienstleistungen : Kapazitätswirtschaft in virtualisierten Rechenzentren
No full textMagdeburg, Univ., Fak. für Informatik, Diss., 2009Alexander Pinno
A Capacity Supply Model for Virtualized Servers
No full textThis paper deals with determining the capacity supply for virtualized servers. First, a server is modeled as a queue based on a Markov chain. Then, the effect of server virtualization on the capacity supply will be analyzed with the distribution function of the server load.Markov chain, Weibull distribution, server, capacity supply
A Capacity Supply Model for Virtualized Servers
No full textThis paper deals with determining the capacity supply for virtualized servers. First, a server is modeled as a queue based on a Markov chain. Then, the effect of server virtualization on the capacity supply will be analyzed with the distribution function of the server load
