45,715 research outputs found
The Blotted Line | An Interview with Alexa Huang
No full texthttp://theshakespearestandard.com/blotted-line-interview-alexa-huang-professor-english-theatre-east-asian-languages-literatures-international-affairs-george-washington-university-washington-d-c/ "I am proud to have answered my calling to tell stories and to show others how to listen for silenced voices. Story-telling makes us human because it helps us understand the human condition in different contexts. For all my life, I have been looking for a place to call home, which is why I became interested in how narratives are transformed when they move across boundaries of all kinds.
Peritoneal <i>M. corti</i> labeled with hydrazide Alexa 488.
No full text<p>Nuclear stain is blue. (A) After periodate labeling, <i>M. corti</i> distal cytoplasm (dc) is highly fluorescent. Other areas of the parasite showed low to no fluorescent signal. 400× (B) DIC image of A confirming that hydrazide-Alexa 488 is coupled to molecules in the dc. 400× (C) Parasites labeled with the INFLUX kit show fluorescent signal in the proximal cytoplasm (arrowheads) but not in the dc. 200× (D) DIC image of C confirming that molecules labeled with the INFLUX kit are in the proximal cytoplasm and not in the dc. Inset 1 is showing that hydrazide-Alexa 488 labeled molecules are present in the proximal cytoplasm (large arrowheads), but not in the calcareous corpuscles (small arrowheads) or dc. Inset 1 is a DIC and fluorescence image 2.5 times magnified.</p
The construction of Karen Karnak: The multi-author-function
No full textThis thesis is situated within the comparatively recent developments of Web 2.0 and the emergence of interactive WikiMedia, and explores the mode of authorship within a Read/Write culture compared to that of a Read/Only tradition. The hypothesis of this study is that the role of the audience has become merged with the author, and as such, represents new functions and attributes, distinct from a more conventional concept of authorship, in which the roles of audience and author are more separate. Read/Write and participatory culture, as defined by this study, is focused on collaboration, and includes the influences of D.I.Y. culture, Open-Source practices and the production of text by multiple authors. Multi-authorship presents a re-thinking of several concepts which support the notion of the individual author, since the focus of multi-authorship is not on attribution and ownership of a finished text, but on the continued malleability of a text. Modes of multi-authorship, demonstrated in the use of the pseudonyms Alan Smithee and Karen Eliot, represent declarative authors whose names signify multiple origins, whilst concurrently indicating a distinct body of work. The function of these names form an important context to this study, since primary research involves the construction of an experimental mode of multi-authorship utilising WikiMedia technology and the interaction of thirty nine participants, who are invited to create a body of work under the collective pseudonym Karen Karnak. The data generated by this experiment is analysed using aspects of Michel Foucault's author-function to identify and determine power structures inherent in the WikiMedia context. The interplay of power structures, including concepts such as identity, ownership and the body of work, affect the resulting mode of authorship and contribute to the construction of Karen Karnak, suggesting further areas of research into the emerging multi-author
Alexa Fluor 488 negativity in the <i>L1CAM</i> assay represents <i>L1CAM</i> disruption by indels.
No full textSK-N-BE(2) cells were transfected with a Cas9 nuclease targeted to L1CAM (ex26-1), and the L1CAM protein on the surface of transfected cells was labeled with Alexa Fluor 488. Cells were then FCM-sorted to isolate Alexa Fluor 488-positive and -negative cells. Next, a genomic region spanning the edited site at the L1CAM intron 25–exon 26 boundary was PCR-amplified using gDNAs extracted from the Alexa Fluor 488-positive and -negative cells as templates. Lastly, the amplified PCR products were cloned into a plasmid, and multiple plasmids containing the PCR products as inserts were isolated and sequenced. (A) DNA sequences of PCR products amplified from Alexa Fluor 488-positive cells. Sequences are shown in alignment with a wild-type control derived from parental SK-N-BE(2) cells displayed at the top. A red letter indicates an inserted nucleotide. (B) Representative sequencing chromatogram obtained in the analysis shown in (A). (C) DNA sequences of PCR products amplified from Alexa Fluor 488-negative cells displayed in a manner similar to (A). A green letter indicates a substituted nucleotide. (D) Two representative sequencing chromatograms obtained in the analysis shown in (C). (E) L1CAM genotypes in Alexa Fluor 488-positive and -negative cells determined based on the experimental results shown in (A)–(D). In (A)–(D), the vertical dotted lines in red indicate a genomic site cleaved by ex26-1. WT, wild-type; ins, insertion; del, deletion; subst, substitution. (PDF)</p
Nuclis temàtics per a una geografia crítica: les propostes de D.R. Stoddart i D. Harvey
No full textL�autor introdueix el concepte de geografia crítica i comenta dues reflexions sobre la geografia.
Les aportacions de D. R. Stoddart a Berkeley el 1986 i D. Harvey a Heidelberg el 2004. Llurs
propostes propugnen una geografia crítica compromesa amb el coneixement i amb la solució dels
problemes ambientals i socials del seu temps.The author introduces the concept of critical geography and discusses two reflections on geography.
The contributions of D. R. Stoddart at Berkeley in 1986 and D. Harvey at Heidelberg in
2004. Their proposals advocate a critical geography committed with the knowledge and the solution
of environmental and social problems of his time
Staining of oocysts with a mouse anti-<i>Cryptosporidium</i> Alexa Fluor 488 monoclonal antibody.
No full text(A to E) Representation of an Alexa 488 stained sample containing 104 oocysts. (A) A representative plot of oocysts purified from mouse intestine. The oval Oocyst gate encompasses the expected location of the C. parvum oocysts on the plot of SSC-A versus FSC-A based on a positive control of purified oocysts. Histograms showing the distribution of events positive for Alexa Fluor 488 emission on the ungated sample (B) and the Oocyst gate (D). (C) A pseudo-coloured dot plot of FSC-H versus Alexa 488 with the recommended gate for Alexa 488+ oocysts. (E) Overlay of Alexa 488+ oocysts from panel C (blue) and negative events (red) of the ungated sample.</p
FIGURE 1. Alexa duckeana G.S in Alexa duckeana (Leguminosae-Papilionoideae): a new species from the Brazilian Amazon
No full textFIGURE 1. Alexa duckeana G.S. da Silva & Mansano. A. Flowering branch. B. Detail of leaflet blade base. C. Inflorescence with flowers in anthesis. D. Calyx with lobe details. E. Flower with the petals and stamens removed, showing the gynoecium. F. The external face of a petal. G. Stamen with detail of the filament insertion on the anther (dorsifixed). H. Gynoecium showing the indumentum of the ovary. I. Fruit demonstrating the persistent calyx. (A–E and I drawn from: Silva G.S. 528; F-H and J from: Silva G.S. 533). Illustrated by Marcus Falcão.Published as part of <i>Silva, Guilherme Sousa Da, Torke, Benjamin M. & Mansano, Vidal De Freitas, 2023, Alexa duckeana (Leguminosae-Papilionoideae): a new species from the Brazilian Amazon, pp. 255-265 in Phytotaxa 629 (3)</i> on page 258, DOI: 10.11646/phytotaxa.629.3.7, <a href="http://zenodo.org/record/10281745">http://zenodo.org/record/10281745</a>
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Alexa Fluor 488 positivity in the <i>L1CAM</i> correction assay represents the reversion of <i>L1CAM</i> mut-2 to a wild-type sequence.
No full textThe SK-N-BE(2)-derived mut-2 reporter clone was transfected with sgRNA #4 and #6 coupled with Cas9 (H840A) and Donor-L1CAM to correct the L1CAM mutation via TPN. The L1CAM protein on the surface of transfected cells was labeled with Alexa Fluor 488, and cells were subjected to FCM-based sorting to isolate Alexa Fluor 488-positive and -negative populations. PCR was then performed to amplify a genomic region spanning the mut-2 site within L1CAM exon 14 in the Alexa Fluor 488-positive and -negative populations. The amplified PCR products were cloned into a plasmid, and multiple plasmids containing the PCR products as inserts were isolated and sequenced. (A) DNA sequences of PCR products amplified from Alexa Fluor 488-positive cells. Sequences are shown in alignment with a wild-type control derived from parental SK-N-BE(2) cells displayed at the top. Arbitrary numbers placed above the aligned sequences indicate the relative positions of nucleotides. Blue shading indicates a wild-type sequence resulting from mut-2 reversion. Green letters indicate substituted nucleotides. (B) Representative sequencing chromatogram obtained in the analysis shown in (A). (C) DNA sequences of PCR products amplified from Alexa Fluor 488-negative cells displayed in a manner similar to (A). Red shading indicates the mut-2 nonsense mutation. (D) Representative sequencing chromatogram obtained in the analysis shown in (C). (E) L1CAM genotypes in Alexa Fluor 488-positive and -negative cells determined based on the experimental results shown in (A)–(D). 1-bp substitutions shown in (A) and (C), probably introduced during genome editing or by PCR errors, are not considered in genotyping L1CAM, because they are located within an intronic sequence distant from the exon–intron boundary. In (A)–(D), the vertical dotted lines in red indicate a genomic site nicked by Cas9 (H840A) coupled with sgRNA #4 or #6. WT, wild-type; mut, mutant. (PDF)</p
Polymicrobial biofilm of <i>A</i>. <i>vaginae</i> and <i>G</i>. <i>vaginalis</i> in different panes.
No full text<p>Confocal laser scanning image with 400 x magnification of polymicrobial biofilm in different panes, A: vaginal epithelial cells DAPI in blue, B: all bacteria, BacUni-1 PNA-probe with Alexa Fluor 555 in yellow, C: <i>A</i>. <i>vaginae</i> specific PNA-probe AtoITM1 with Alexa Fluor 488 in green, D: <i>G</i>. <i>vaginalis</i> specific PNA-probe Gard162 with Alexa Fluor 647 in red (superimposed image can be seen in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136658#pone.0136658.g003" target="_blank">Fig 3A</a>).</p
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