244 research outputs found
On-chip Microdialysis System with Flow-through Glucose Sensing Capabilities
No full textThe published version of this article is available at http://www.journalofdst.org/May2007/pdf/VOL-1-3-ORG3-HSIEH.pd
Non-Invasive Glucose Monitoring: A Novel Approach
No full textBackground: The main concern in noninvasive (NI) glucose measurement is achieving high accuracy readings, although no blood (or other fluid) is involved in the process. Using methods based on different physical properties of a measured object can ensure the independence of each of the readings and therefore improve the validity of the end result. By using a combination of (three) independent technologies—ultrasonic, electromagnetic, and thermal—GlucoTrack™ presents a unique approach for a real-time, truly NI blood glucose spot measurement. Methods: Clinical trials were performed in two stages. Stage 1 was an initial method validation and performance verification of the device. In this stage, 50 type 1 and 2 diabetic patients, as well as healthy subjects, were evaluated with GlucoTrack against Ascensia Elite® (Bayer). In the second stage, 85 additional diabetic subjects were evaluated in half and full daytime sessions using a GlucoTrack comparison with HemoCue® (Glucose 201+). Results: A total of 135 subjects were tested during the trial period, producing 793 data pairs. Using Clarke error grid analysis, 92% of the readings fell in the clinically acceptable zones A and B, with 50% in the A zone. Mean and median relative absolute differences were 29.9 and 19.9%, respectively. Conclusions: Integrating several modalities for NI assessment of glucose level enables more accurate readings, while a possible aberration in one modality is bypassed by the others. The present generation of GlucoTrack gives promising results; however, further improvement of the accuracy of the device is needed.The published version of this article is available at http://www.journalofdst.org/amember/plugins/protect/new_rewrite/login.php?v=-any&url=/March2009/Articles/VOL-3-2-SYM4-HARMAN-BOEHM.pdf%3
Hieracium murorum subsp. cophogonium Bornm. & Zahn
No full textHieracium murorum subsp. cophogonium Bornm. & Zahn in Zahn (1925: 161). Ind. loc.: “Frontière bâloise-badoise: Hohr[sic!] M i hr près Zell (A. Krafft). Trouvé aussi par Bornmüller à Tr i bsdorf, près Weimar, dans la Thuringe.” Lectotype (designated here by Gottschlich): — GERMANY. Baden-Wuerttemberg: Hohe M i hr, 11 June 1923, A. Krafft (BREG!). — Remaining syntype: GERMANY. Thuringia: Weimar, Tr i bsdorf, westl. bei d. Bahnbrücke, 8 June 1923, J. Bornmüller (B barcode B 10 0460350!). Remarks: —Although Bornmüller is co-author of the taxon, in the protologue the collection of Krafft is cited first and is here selected as lectotype.Published as part of Vogt, Robert & Gottschlich, Günter, 2023, Type material in the Hieracium (Compositae: Cichorieae) collection of Joseph Bornmüller, pp. 81-126 in Phytotaxa 613 (2) on page 102, DOI: 10.11646/phytotaxa.613.2.1, http://zenodo.org/record/834553
European enlargement and the economic crisis : impact and lasting effects
No full textThis working paper by Rebecca Zahn looks at the effects of the economic crisis on the enlarged European Union and the European Social Model. Starting from an analysis of the well-known Viking and Laval decisions of the European Court of Justice from 2007 and 2008, the author of the report sees increasing tensions between EU member states over “social dumping”, austerity packages and growing inequality between workers. This development results in citizens questioning the benefits of further European integration and threatens the very existence of the European social model
Three hundred eighty thousand year long stable isotope and faunal records from the Red Sea : influence of global sea level change on hydrography
No full textStable isotope and faunal records from the central Red Sea show high-amplitude oscillations for the past 380,000 years. Positive δ18O anomalies indicate periods of significant salt buildup during periods of lowered sea level when water mass exchange with the Arabian Sea was reduced due to a reduced geometry of the Bab el Mandeb Strait. Salinities as high as 53‰ and 55‰ are inferred from pteropod and benthic foraminifera δ18O, respectively, for the last glacial maximum. During this period all planktonic foraminifera vanished from this part of the Red Sea. Environmental conditions improved rapidly after 13 ka as salinities decreased due to rising sea level. The foraminiferal fauna started to reappear and was fully reestablished between 9 ka and 8 ka. Spectral analysis of the planktonic δ18O record documents highest variance in the orbital eccentricity, obliquity, and precession bands, indicating a dominant influence of climatically - driven sea level change on environmental conditions in the Red Sea. Variance in the precession band is enhanced compared to the global mean marine climate record (SPECMAP), suggesting an additional influence of the Indian monsoon system on Red Sea climates
Hieracium lachenalii var. pseudoperscissum Zahn 1934
No full text<i>Hieracium lachenalii</i> var. <i>pseudoperscissum</i> Zahn (1934: 540). <p>Ind. loc.: “ Thüringen: Wald am Ruppberg bei Zella → Mehlis (Bornmüller)!”</p> <p> <b>Lectotype (designated here by Gottschlich):</b> — GERMANY. Thuringia: a: Thür. Wald, Zella-Mehlis, Ruppberg, Laubwald, 650 m, 20 July 1928, <i>J. Bornmüller</i>; b: Thür. Wald: Zella-Mehlis, Ruppberg, bei der Oberf i rsterei, 20 July 1928, <i>J. Bornmüller</i> (B barcode B 10 0460357!; isolectotype: GERMANY. Thuringia: Thür. Wald: Zella-Mehlis, am Ruppberg, ca. 6–700 m, 20 July 1928, <i>J. Bornmüller</i> (B barcode B 10 0460358!).—Current name: <i>Hieracium lachenalii</i> subsp. <i>lachenalii</i>.</p> <p> <b>Remarks:</b> —The lectotype bears two labels (a+b). On label “a” Zahn noted the determination. Label “b” seems to be a handwritten copy by Bornmüller. Author and type of <i>Hieracium lachenalii</i> have changed, therefore the current name is <i>H. lachenalii</i> Suter (1802: 145) (subsp. <i>lachenalii</i>). The characters of the variety fall into the phenotypic variation of the type.</p>Published as part of <i>Vogt, Robert & Gottschlich, Günter, 2023, Type material in the Hieracium (Compositae: Cichorieae) collection of Joseph Bornmüller, pp. 81-126 in Phytotaxa 613 (2)</i> on page 96, DOI: 10.11646/phytotaxa.613.2.1, <a href="http://zenodo.org/record/8345535">http://zenodo.org/record/8345535</a>
Continuous-flow, electrically-triggered, single cell-level electroporation
No full textElectroporation creates transient openings in the cell membrane, allowing for intracellular delivery of diagnostic and therapeutic substances. The degree of cell membrane permeability during electroporation plays a key role in regulating the size of the delivery payload as well as the overall cell viability. A microfl uidic platform offers the ability to electroporate single cells with impedance detection of membrane permeabilization in a high-throughput, continuous-fl ow manner. We have developed a fl ow-based electroporation microdevice that automatically detects, electroporates, and monitors individual cells for changes in permeability and delivery. We are able to achieve the advantages of electrical monitoring of cell permeabilization, heretofore only achieved with trapped or static cells, while processing the cells in a continuous-fl ow environment. We demonstrate the analysis of membrane permeabilization on individual cells before and after electroporation in a continuous-fl ow environment, which dramatically increases throughput. We have confi rmed cell membrane permeabilization by electrically measuring the changes in cell impedance from electroporation and by optically measuring the intracellular delivery of a fl uorescent probe after systematically varying the electric fi eld strength and duration and correlating the pulse parameters to cell viability. We fi nd a dramatic change in cell impedance and propidium iodide (PI) uptake at a pulse strength threshold of 0.87 kV/cm applied for a duration of 1 ms or longer. The overall cell viability was found to vary in a dose dependent manner with lower viability observed with increasing electric fi eld strength and pulse duration. Cell viability was greater than 83% for all cases except for the most aggressive pulse condition (1 kV/cm for 5 ms), where the viability dropped to 67.1%. These studies can assist in determining critical permeabilization and molecular delivery parameters while preserving viability.Peer reviewe
Speech and debate
No full textMy thesis encompasses all the preparation, processes and self-evaluations involved in directing a full-length play.M.F.A.Includes bibliographical referencesby Claudia Zah
Locally covariant chiral fermions and anomalies
No full textAbstractWe define chiral fermions in the presence of non-trivial gravitational and gauge background fields in the framework of locally covariant field theory. This allows to straightforwardly compute the chiral anomalies on non-compact Lorentzian spacetimes, without recourse to a weak field approximation
Numerical modeling of microfluidic two-phase electrohydrodynamic instability:
No full textOrganic-aqueous liquid (phenol) extraction is one of many standard techniques to efficiently purify DNA directly from cells. Effective dispersion of one fluid phase in the other increases the surface area over which biological component partitioning may occur, and hence enhances DNA extraction efficiency. Electrohydrodynamic (EHD) instability can be harnessed to achieve this goal and has been experimentally demonstrated by Zahn and Reddy (2006). In this work, analysis and simulation are combined to study two-phase EHD instability. In the
problem configuration, the organic (phenol) phase flows into the microchannel in parallel with and sandwiched between two aqueous streams, creating a three-layer planar geometry; the two liquid phases are immiscible. An electric field is applied to induce instability and to break the organic stream into droplets. The Taylor-Melcher leaky-dielectric model is employed to investigate this phenomenon. A linear analysis is carried out with a Chebyshev pseudo-spectral method, whereas a fully nonlinear numerical simulation is implemented using a finite volume, immersed boundary method (IBM). The results from both models compare favorably with each other. The linear analysis reveals basic instability characteristics such as kink and sausage modes. On the other hand, the nonlinear simulation predicts surface deformation in the strongly nonlinear regime pertinent to droplet formation. These numerical tools will be used to investigate the effects of the applied electric field, geometry, and convective flow rate on mixing and dispersion. The eventual objective is to maximize surface area of the organic phase under given experimental conditions for optimized DNA extraction.M.S.Includes bibliographical references (p. 101-104)by Venkat raman Thenkarai Narayana
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