1,721,015 research outputs found
Tubular adenoma of the breast - An immunohistochemical study of ten cases
No full textTen cases of so-called ''tubular'' adenoma and six cases of fibroadenoma of the breast have been investigated with an immunohistochemical technique with the aim of providing both more details on their immunophenotype and of ascertaining the possible relationships between tubular adenoma and fibroadenoma. Smooth Muscle Actin, Cytokeratin 14, GFAP, S-100 Protein and Vimentin immunoreactivity have clearly demonstrated that cells with myoepithelial immunophenotype are one of the major cell components in breast adenomas. Epithelial Membrane Antigen (EMA), Human Milk Pat Globule II (HMFG II), Estrogen and Progesterone receptors have been detected in adluminal epithelial cells exclusively. Furthermore, Smooth Muscle Actin and Vimentin highlighted an abundant myofibroblastic component, intermingled with tubular structures in both tumor types. A low percentage (10-22%) of adluminal cells and of myofibroblasts showed Ki-67 immunoreactivity in tubular adenomas and in fibroadenomas, whereas only rare myoepithelial cells demonstrated Ki-67 positivity in both tumor types. These data seem to indicate that several cell components of both epithelial and mesenchymal origin (epithelial cells, myoepithelial cells, myofibroblasts) are involved in the genesis of tubular adenomas. The morphological and immunohistochemical features of tubular adenomas closely resemble, in some areas of the tumors, those of fibroadenoma. Therefore, they may represent histogenetically related neoplasms with exuberant ductular component in tubular adenomas and predominant stromal component in fibroadenoma
Functionless non chromaffin retroperitoneal paraganglioma causing cauda equina compression
No full textDifferential glycopattern in the horse pelvic and penile urethra epithelium
No full textThe urethra of the male has a dual function acting as a route for urine and semen. Often the male urethra corresponds to the preferntial entrance for pathogenic agents that can affect both the...
Glycan profiles of the male donkey urethra
No full textThe urethra of the male has a dual function, acting as a route foe urine and seme
Expression of heat shock proteins 60, 70, 90 in equine granulosa cells
No full textIt is well known that heat shock proteins (HSPs) are not only mediators of heat stress but also regulators of cell proliferation (1) differentiation (2) and apoptosis (3). During the equine..
EXPRESSION OF L-TYPE VOLTAGE CALCIUM CHANNELS IN EQUINE GRANUOSA CELLS: A MOLECULAR AND FUNCTIONAL STUDY
No full textThe ovary is dependent on pituitary gonadotropins to ensure folliculogenesis which in turn relies on intracellular calcium signalling, induced by follicular stimulating hormone to promote oocyte activation and maturation [1]. The intracellular calcium concentration [Ca2+]I rise is due in part to L-voltage-gated calcium channels (L-VOCCs). The role of L-VOCCs in the ovarian follicular dynamic has been assessed in the granulosa cells (GCs) of several animals [2] but not in the mare, whose follicular growth is primarily controlled by the changing day length that drives the reproductive seasonality. This study aims to evaluate the presence, quantification, and localization of L-VOCCs in equine GCs recovered from follicles of different diameters and their involvement in modulating [Ca2+]i during follicles development. GCs were recovered from follicles of ovaries obtained during the year from a local slaughterhouse. An average of 5±1 mares were analyzed each month. Based on the cyclic periodicity, the number of follicles varied. Follicles were categorized as small, medium, and large [3]. GCs from each type of size-follicle were pooled and processed by a) western blot, to assess the expression and quantify L-VOCCs employing a rabbit affinity-purified primary antibody against the α1 subunit of L-VOCCs and a biotinylated universal secondary antibody (Vectastain Elite ABC Universal Kit, Burlingame, USA). The positive hybridization was revealed by a DAB substrate kit (Vector-Laboratories, Burlingame, USA); b) immunohistochemistry, to localize L-VOCCs by the same primary antibody used in the western blot procedure and a FITC-conjugated secondary antibody; c) fluorescent measurement of L-VOCCs activity and cytosolic Ca2+ dynamics employing Fura-2-AM fluorescent dye and Bay k-8644 and Nifedipine, which are agonist and antagonist, respectively, of the L-VOCCs using a QuantiCell 900 integrated image system (VisiTech International, Sunderland, UK). Data were analyzed by parametric tests. Results showed that all categories of GCs expressed L-VOCCs, which, as aspected, appeared as doublet bands at different molecular weights. Fluorescence immunohistochemistry evidenced positive signals at the surface of GCs. The quantification of the L-VOCC level showed that small follicles increased their expression in February (January vs February, P<0.05), just before the beginning of the equine breeding season. Moreover, it has been found that the used agonist and antagonist of the L-VOCCs induced a significant (P<0.001) change in the [Ca2+]i in follicles of all sizes. Large follicles resulted in a greater change in [Ca2+]I after addition of Bayk K-8644 or Nifedipine when compared to the basal condition. The results obtained indicate the existence of a regulatory system of the function of the L-VOCCs associated with the functional state of the follicle during its growth and maturation
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