1,721,030 research outputs found
A Guide to the Variability of Flavonoids in Brassica oleracea
Flavonoids represent a typical secondary metabolite class present in cruciferous vegetables. Their potential as natural antioxidants has raised considerable scientific interest. Impacts on the human body after food consumption as well as their effect as pharmaceutical supplements are therefore under investigation. Their numerous physiological functions make them a promising tool for breeding purposes. General methods for flavonoid analysis are well established, though new compounds are still being identified. However, differences in environmental circumstances of the studies and analytical methods impede comparability of quantification results. To promote future investigations on flavonoids in cruciferous plants we provide a checklist on best-practice in flavonoid research and specific flavonoid derivatives that are valuable targets for further research, choosing a representative species of scientific interest, Brassica oleracea
Lyperia tristis
<i>3.7. Lyperia tristis</i> <p> Dry material from the voucher (1080 mg) was blended with hot EtOH (10 ml), left to stand for 8 days, and treated as above to give after concentration 67 mg residue. The 1 H NMR data were obtained directly from this extract, re-dissolved in CD 3 OD (see SI). The presence of aucubin (<b>2</b>) and melittoside (<b>3</b>) could be confirmed.</p>Published as part of <i>Gousiadou, Chryssoula, Kokubun, Tetsuo, Albach, Dirk C., Gotfredsen, Charlotte H. & Jensen, Søren Rosendal, 2019, Iridoid glucosides in the genus Sutera (Scrophulariaceae) as chemotaxonomic markers in tribe Limoselleae, pp. 149-155 in Phytochemistry 158</i> on page 154, DOI: 10.1016/j.phytochem.2018.10.021, <a href="http://zenodo.org/record/10481450">http://zenodo.org/record/10481450</a>
Microdon dubius
<i>3.8. Microdon dubius</i> <p> Dry material from the voucher (720 mg) was treated as under 3.7 to give after concentration 47 mg residue. The 1 H NMR data (see SI) were obtained directly from this extract, re-dissolved in CD 3 OD</p>Published as part of <i>Gousiadou, Chryssoula, Kokubun, Tetsuo, Albach, Dirk C., Gotfredsen, Charlotte H. & Jensen, Søren Rosendal, 2019, Iridoid glucosides in the genus Sutera (Scrophulariaceae) as chemotaxonomic markers in tribe Limoselleae, pp. 149-155 in Phytochemistry 158</i> on page 154, DOI: 10.1016/j.phytochem.2018.10.021, <a href="http://zenodo.org/record/10481450">http://zenodo.org/record/10481450</a>
Landscape complexity has limited effects on the genetic structure of two arable plant species, Adonis aestivalis and Consolida regalis
The agricultural landscape of central Europe has changed dramatically in recent decades due to intensified cultivation, bringing many of its characteristic species to the brink of extinction. We investigated whether landscape structure affects the genetic structure and diversity of remnant populations of the two arable plant species Adonis aestivalis and Consolida regalis. We used dominant amplified fragment length polymorphism markers (AFLPs) and compared populations from six regions of 5 km² in central Germany. These regions represent two different classes of landscape structural complexity: intensively used, homogeneous landscapes (>95% of area covered by arable land with low extent of field margins) or heterogeneous regions (<60% of area covered by arable land with large extent of field margins). Contrary to expectations, within-population diversity levels did not significantly differ between homogeneous and heterogeneous landscapes. No significant isolation-by-distance was found for either species, regardless of landscape structure, and genetic structures may still mirror more continuous conditions before large-scale restructuring commenced in Germany's arable landscapes from the 1950s onwards. These results suggest that current landscape complexity, as such, is not as important for local-level genetic structure for the species studied. Thus, homogenised and intensively used landscapes may also be important for the conservation of arable plant diversity and should not be neglected
Melanospermum transvaalense
<i>3.6. Melanospermum transvaalense</i> <p> Dry whole plants (11 g) were blended with hot EtOH (100 ml), left to stand for 11 days, and treated as above to give after concentration 1.90 g residue. Chromatography gave: sugars (140 mg), aucubin (<b>2</b>, 10 mg), impure geniposidic acid (<b>7</b>, 20 mg), impure mussaenosidic acid (<b>6</b>, 10 mg), bartsioside (<b>1</b>, 25 mg), plantarenaloside (<b>5</b>, 380 mg) and verbascoside (<b>15</b>, 40 mg).</p>Published as part of <i>Gousiadou, Chryssoula, Kokubun, Tetsuo, Albach, Dirk C., Gotfredsen, Charlotte H. & Jensen, Søren Rosendal, 2019, Iridoid glucosides in the genus Sutera (Scrophulariaceae) as chemotaxonomic markers in tribe Limoselleae, pp. 149-155 in Phytochemistry 158</i> on page 153, DOI: 10.1016/j.phytochem.2018.10.021, <a href="http://zenodo.org/record/10481450">http://zenodo.org/record/10481450</a>
Sutera foetida
<i>3.4. Sutera foetida</i> <p> Fresh plants (81 g) were blended with EtOH (300 ml) and treated as above to give after concentration 2.23 g residue. Chromatography gave: sugars (1.1 g), a fraction with mainly cachineside I (<b>9</b>, 14 mg), secologanoside (<b>14</b>, 18 mg), sinapoyl glucoside (<b>20</b>, 24 mg), 5-deoxystansioside (<b>8</b>, 230 mg), 7-deoxyloganic acid (<b>10</b>, 35 mg), verbascoside (<b>15</b>, 65 mg) and a fraction (90 mg) which was further separated with repeated C 18 HPLC first with linear gradient of MeOH–H 2 O, 40:60–100:0 over 20 min, <i>t</i> R 17.3 min, then with MeCN-H 2 O, 35:65, isocratic, <i>t</i> R 12.3 min, to give pure suterolide (<b>21</b>, 2.3 mg).</p>Published as part of <i>Gousiadou, Chryssoula, Kokubun, Tetsuo, Albach, Dirk C., Gotfredsen, Charlotte H. & Jensen, Søren Rosendal, 2019, Iridoid glucosides in the genus Sutera (Scrophulariaceae) as chemotaxonomic markers in tribe Limoselleae, pp. 149-155 in Phytochemistry 158</i> on page 153, DOI: 10.1016/j.phytochem.2018.10.021, <a href="http://zenodo.org/record/10481450">http://zenodo.org/record/10481450</a>
Lyperia (Sutera) antirrhinoides
<i>3.3. Lyperia (Sutera) antirrhinoides</i> <p> Frozen plants (100 g) were blended with EtOH (300 ml) filtered and taken to dryness, and partitioned between H 2 O and Et 2 O. The aqueous phase was concentrated (1.6 g) and subjected to C 18 reverse-phase chromatography (Lobar size C), eluting with MeOH–H 2 O mixtures. Aucubin (<b>2</b>) and melittoside (<b>3</b>) was eluted with 25:1 while 8- <i>O-</i> acetylharpagide (<b>4</b>) was eluted with 3:1. Unfortunately the amounts of the isolated compounds were not recorded. NMR spectra of the compounds are given in SI.</p>Published as part of <i>Gousiadou, Chryssoula, Kokubun, Tetsuo, Albach, Dirk C., Gotfredsen, Charlotte H. & Jensen, Søren Rosendal, 2019, Iridoid glucosides in the genus Sutera (Scrophulariaceae) as chemotaxonomic markers in tribe Limoselleae, pp. 149-155 in Phytochemistry 158</i> on page 153, DOI: 10.1016/j.phytochem.2018.10.021, <a href="http://zenodo.org/record/10481450">http://zenodo.org/record/10481450</a>
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Sutera cordata
<i>3.5. Sutera cordata</i> <p> Fresh plants (43 g) were blended with EtOH (200 ml) and treated as above to give after concentration 1.57 g residue. Chromatography gave: a fraction containing mainly secologanoside (<b>14</b>, 520 mg), a fraction with mainly secologanic acid (<b>13</b>, 70 mg), a mixture (1:1) of sweroside (<b>12</b>) and villoside (<b>11</b>) (33 mg), echinacoside (<b>16</b>, 22 mg), followed by fractions (total 300 mg) which were repeatedly chromatographed over C 18 HPLC (MeCN-H 2 O) to yield: tubuloside A (<b>18</b>, MeCN–H 2 O 15:85, 2 mg) and 2′′′′-acetyl angoroside A (<b>19</b>, MeCN–H 2 O 21:79, 6 mg), both in a semi-pure state. Villoside (<b>11</b>, 5.9 mg) and sweroside (<b>12</b>, 6.0 mg) were separated by HPLC with MeCN-H 2 O (20:80). Pure samples of (<b>13</b>, 20.8 mg) and (<b>14</b>, 5.2 mg) were also obtained with MeCN-H 2 O gradient (17:83 → 85:15, v/v, over 15 min).</p>Published as part of <i>Gousiadou, Chryssoula, Kokubun, Tetsuo, Albach, Dirk C., Gotfredsen, Charlotte H. & Jensen, Søren Rosendal, 2019, Iridoid glucosides in the genus Sutera (Scrophulariaceae) as chemotaxonomic markers in tribe Limoselleae, pp. 149-155 in Phytochemistry 158</i> on page 153, DOI: 10.1016/j.phytochem.2018.10.021, <a href="http://zenodo.org/record/10481450">http://zenodo.org/record/10481450</a>
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