122 research outputs found
An, In Vitro, Study of some Pesticides on the Activity of Human Serum Paraoxonase (PON1)
No full textalayli, azize/0000-0002-7568-6970;WOS: 000415540000008Paraoxonase-1 (PON1) is a high density lipoprotein (HDL)-associated enzyme capable of hydrolyzing diverse substrates from organophosphate (OP) toxins to oxidized phospholipids. In this study, the effects of dichlorvos, parathion-methyl, dimethoate, chlorpyrifos methyl, chlorpyrifos ethyl, acetamiprid, mancozeb, lufenuron and trifluralin which are pesticides, were investigated on human serum paraoxonase as in vitro. While trifluralin, mancozeb and methyl parathion enhanced the PON activity, lufenuron, dimetoate and chlorpyrifos methyl and chlorpyrifos ethyl firstly activated and then they inhibited in the different range of enzyme activity. It was determined that acetamidine and dichlorvos were inhibited and the CI50 values of the chemicals caused inhibition were calculated by means of activity percentage-[I] diagrams. The values of CI50 were 38,19 mM and 13,49 mM for PON1 respectively. Changes of PON enzyme's kinetic parameters were most important during pesticide metabolism, because pesticides were risk for human health
Democracy Sausage with Mark Kenny: Saving the Australian dream
No full textMark and Marija discuss Scott Morrison's exit from politics, before housing affordability campaigner Maiy Azize joins us to propose solutions to Australia's housing crisis.
Will Australia's political leaders have the ambition and ability to sell substantial housing policy changes to the public? What might a fairer system look like? And how will Scott Morrison fashion his career post-parliament? On this episode of Democracy Sausage, Maiy Azize, author of a new report recommending changes to tax and investment policies for housing, joins Professor Mark Kenny and Marija Taflaga to discuss the policy and politics of Australia’s housing crisis.
Maiy Azize is the Deputy Director of the Anglicare Australia and spokesperson for Everybody’s Home, a national campaign that aims to fix the housing crisis
Effects of Zinc, Copper and Iron Oxide Nanoparticles on Induced DNA Methylation, Genomic Instability and LTR Retrotransposon Polymorphism in Wheat (Triticum aestivum L.)
No full textNanomaterials with unique and diverse physico-chemical properties are used in plant science since they improve plant growth and development and offer protection against biotic and abiotic stressors. Previous studies have explored the effects of such nanomaterials on different plant mechanisms, but information about the effects of nanomaterials on induced DNA methylation, genomic instability and LTR retrotransposon polymorphism in wheat is lacking. Therefore, the present study highlights the key role of nanoparticles in DNA methylation and polymorphism in wheat by investigating the effects of ZnO, CuO and γ-Fe3O4 nanoparticles (NPs) on mature embryo cultures of wheat (Triticum aestivum L.). Nanoparticles were supplemented with Murashige and Skoog (MS) basal medium at normal (1X), double (2X) and triple (3X) concentrations. The findings revealed different responses to the polymorphism rate depending on the nanoparticle type and concentration. Genomic template stability (GTS) values were used to compare the changes encountered in iPBS profiles. ZnO, CuO and γ-Fe3O4 NPs increased the polymorphism rate and cytosine methylation compared to the positive control while reducing GTS values. Moreover, non-γ-Fe3O4 NPs treatments and 2X ZnO and CuO NP treatments yielded higher polymorphism percentages in both MspI- and HpaII-digested CRED-iPBS assays and were thus classified as hypermethylation when the average polymorphism percentage for MspI digestion was considered. On the other hand, the 3X concentrations of all nanoparticles decreased HpaII and MspI polymorphism percentages and were thus classified as hypomethylation. The findings revealed that MS medium supplemented with nanoparticles had epigenetic and genotoxic effects
Ce<sub>2</sub>O<sub>3</sub> nanoparticle synthesis, characterization, and application to callus formation and plant regeneration from mature embryo culture of wheat (<i>Triticum aestivum</i> L.)
No full textCerium oxide nanoparticles (Ce2O3-NPs) are widely used for their catalytic and oxidative properties, such as in diesel additives and potential oncology treatments. However, limited data exists on their impact on callus formation and plant regeneration in wheat. This study investigates the effects of different genotypes and Ce2O3-NPs concentrations on callus formation and plant regeneration in wheat (Triticum aestivum L.) using mature embryo culture. Our hypothesis was that Ce2O3-NPs would enhance callus and plant regeneration rates in a genotype-dependent manner. We used Murashige and Skoog (MS) medium with varying concentrations of Ce2O3-NPs (1-7 mg/L) to assess their effects. The K & imath;rik genotype showed the highest callus formation (7 mg/L Ce2O3-NPs), while the Rumeli genotype showed the lowest (1 mg/L Ce2O3-NPs). Embryogenic callus formation was the highest in K & imath;rik without Ce2O3-NPs and the lowest in Rumeli with 3 mg/L Ce2O3-NPs. Root and shoot formation rates in Rumeli were 16.03% and 51.40%, respectively, compared to 3.94% and 40.49% in K & imath;rik. The main outcomes demonstrated that 1 mg/L Ce2O3-NPs promoted root formation, while 7 mg/L was optimal for shoot formation. Genotypic effects on callus, root, and shoot formation, as well as plant regeneration, were significant. MS media with Ce2O3-NPs enhanced callus formation and regeneration in wheat tissue culture, highlighting the importance of genotype in these processes. Future research should explore the underlying mechanisms of Ce2O3-NPs' effects on plant tissue culture and extend the study to other plant species to validate these findings
Highly sensitive glucose sensor based on ZnO NPs as a biomimetic enzyme
No full textResearch Development Center of Ataturk UniversityAtaturk University; Ataturk University, TurkeyAtaturk University; [FAD-2018-6321]This research has been performed under the project numbered FAD-2018-6321 and supported by the Research Development Center of Ataturk University. The authors acknowledged the support of Ataturk University, Turkey, for this work. The authors, Hayrunnisa Nadaroglu and Azize Alayli Gungor, hereby confirm that this manuscript is performed according to guidelines and has not already been published nor is it under consideration for publication elsewhere. This article does not contain any studies with human or animal subjects.In this work, the development of zinc biosensors based on zinc nanoparticles (Zn NPs) biomimetic enzymes were carried out to spectrophotometrically detect glucose in food samples. Briefly; Glucose oxidase enzyme reacts with glucose in food samples to form gluconic acid and H2O2. Behind; the peroxide formed can be measured spectrophotometrically using ABTS substrate with the enzyme mimic effect of Zn NPs. In this way, the amount of glucose was determined by a simple, effective and selective method by measuring the change in absorbance of the reaction medium, which changed by oxidation. ZnO NPs were obtained from Zn(NO3)(2) by green synthesis method using raw fig extract. Then, the amount of glucose in some food samples was determined using ZnO NPs. The results showed that the glucose content in the samples was exactly and reproducibly measured with a correlation coefficient of 0.9812 at 3.47 mM-27.78 mM concentrations. This will greatly simplify the design and manufacture of the new biosensor, which can be used for glucose fixation in food samples in an inexpensive, efficient, environmentally friendly and fast manner
Esra Turgut, Sümeyra Gündüz, Hayrunnisa Nadaroğlu, Azize Alaylı, Kitosan Ayçiçeği ve Kitosan Ayçiçeği Nanodemir ile Atık Sulardan Metil Oranj Boyasının Uzaklaştırılması
No full text
Investigation of Some Industrial Microbial Enzymes and Immobilization onto Nanomaterials Thereof
No full textHighly sensitive glucose sensor based on ZnO NPs as a biomimetic enzyme
No full textIn this work, the development of zinc biosensors based on zinc nanoparticles (Zn NPs) biomimetic enzymes were carried out to spectrophotometrically detect glucose in food samples. Briefly; Glucose oxidase enzyme reacts with glucose in food samples to form gluconic acid and H2O2. Behind; the peroxide formed can be measured spectrophotometrically using ABTS substrate with the enzyme mimic effect of Zn NPs. In this way, the amount of glucose was determined by a simple, effective and selective method by measuring the change in absorbance of the reaction medium, which changed by oxidation. ZnO NPs were obtained from Zn(NO3)(2) by green synthesis method using raw fig extract. Then, the amount of glucose in some food samples was determined using ZnO NPs. The results showed that the glucose content in the samples was exactly and reproducibly measured with a correlation coefficient of 0.9812 at 3.47 mM-27.78 mM concentrations. This will greatly simplify the design and manufacture of the new biosensor, which can be used for glucose fixation in food samples in an inexpensive, efficient, environmentally friendly and fast manner
Green synthesis of gold nanoparticles using quail egg yolk and investigation of potential application areas
No full textNanotechnology is a growing area of research. For example, gold nanoparticles have a wide variety of applications, including optical, electronic, and medical; they are also used as catalysts in biosensor applications and can be used for scanning, carrier, heat source, and sensors. Therefore, nanoparticle synthesis is of great importance to the country's economy and to scientific development. Nanoparticle synthesis involves using chemical methods, physical methods, or both chemical and physical methods. The synthesis is performed at high pressures or high temperatures and severe conditions, both of which have a high cost in terms of energy. In our investigation, we used a green synthesis method, which used quail eggs that required more moderate conditions and less energy. The yolk from quail eggs has a high protein and vitamin content. Using quail egg yolks, the reaction conditions were optimized in terms of pH, temperature, and concentration. The morphological properties of the obtained gold nanoparticles were characterized using an ultraviolet-visible spectrophotometer, a scanning electron microscope, and an X-ray diffraction analysis
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