725 research outputs found
Technical tip: high-resolution isolation of nanoparticle–protein corona complexes from physiological fluids
No full textNanoparticles (NPs) in contact with biological fluids are generally coated with environmental proteins, forming a stronger layer of proteins around the NP surface called the hard corona. Protein corona complexes provide the biological identity of the NPs and their isolation and characterization are essential to understand their in vitro and in vivo behaviour. Here we present a one-step methodology to recover NPs from complex biological media in a stable non-aggregated form without affecting the structure or composition of the corona. This method allows NPs to be separated from complex fluids containing biological particulates and in a form suitable for use in further experiments. The study has been performed systematically comparing the new proposed methodology to standard approaches for a wide panel of NPs. NPs were first incubated in the biological fluid and successively recovered by sucrose gradient ultracentrifugation in order to separate the NPs and their protein corona from the loosely bound proteins. The isolated NP–protein complexes were characterized by size and protein composition through Dynamic Light Scattering, Nanoparticle Tracking Analysis, SDS-PAGE and LC-MS. The protocol described is versatile and can be applied to diverse nanomaterials and complex fluids. It is shown to have higher resolution in separating the multiple protein corona complexes from a biological environment with a much lower impact on their in situ structure compared to conventional centrifugal approaches
Grandidierella longidactylus Ledoyer
No full textGrandidierella longidactylus Ledoyer Grandidierella longidactylus Ledoyer, 1978. Material examined. One male, Baie aux Huîtres, muddy sand, 1.5 m, 10 September 2001, no GPS reading. Coll. A. Mackie. Distribution. Mascarene endemic.Published as part of Myers, Alan, 2004, Amphipoda (Crustacea) of the family Aoridae (Corophiidea) from Rodrigues, Indian Ocean, pp. 3123-3135 in Journal of Natural History 38 (23) on page 3134, DOI: 10.1080/00222930410001695079, http://zenodo.org/record/467545
Young people, youth work and social justice: a participatory parity perspective
No full textThis thesis explores issues of social injustice impacting on a sample group of young
people living in a Scottish community and critically examines their experiences on the
periphery of the labour market. Existing research evidence has highlighted myriad
issues impacting on young people as they struggle to make the transition to adulthood.
Young people in the UK have been particularly impacted by the economic turbulence of
recent years with stagnating wages, higher rates of unemployment compared to older
age groups, an increase in precarious employment and a gradual erosion of welfare
entitlement. Allied to this, unemployed youth continue to be disparaged in popular
discourse, labelled amongst other things as feckless and idle. As a consequence, there is
evidence that young people on the margins of society are disengaging from formal
politics, feeling alienated from an arena that they also see as disconnected from their
everyday lives.
This thesis uses the framework of social justice as conceived by Nancy Fraser to
critically analyse perceived injustices affecting the lives of young people. These issues
manifest across all three spheres of injustice as identified by Fraser; the economic, the
cultural and the political spheres of social life - what she calls the domains of
redistribution, recognition and representation, respectively. The findings of my
research study confirms that Fraser’s framework not only allows us to bring together
the multiple injustices impacting on these young people’s lives, but helps to reveal the
ways in which they overlap and interpenetrate, reinforcing marginalisation.
Fraser’s framework is also utilised as a lens through which to analyse and understand
the context within which practitioners working with the young people are operating. As
many writers in the area of youth work argue, it is an ethical requirement that
the practice supports young people towards addressing any injustices impacting
on their lives. This study finds that the ability of practitioners to respond to the issues of
injustice in the lives of the young people is compromised by a performative landscape
centred on meeting pre-ordained targets and outcomes
Holocene glacier history of Frank Mackie Glacier, northern British Columbia Coast Mountains
No full textFrank Mackie Glacier repeatedly advanced across the Bowser River valley in northwestern British Columbia to impound Tide Lake during the Holocene. The most recent infilling of Tide Lake was associated with a late Little Ice Age glacier advance and ended around 1930 when the lake catastrophically drained. Over the last century Frank Mackie Glacier has retreated and down wasted to reveal multiple glaciogenic sedimentary units within the proximal faces of prominent lateral moraines. The units are separated by buried in-situ tree stumps and laterally contiguous wood mats deposited on paleosols. Dendroglaciological and radiocarbon dating of these wood remains show that Frank Mackie Glacier expanded into standing forests at 3710-3300, 2700-2200, 1700-1290, 900-500, and 250-100 cal. yr BP. These advances coincide closely in time with the previously established Tide Lake glacier dam chronology and with the Holocene history of other glaciers in the Bowser River watershed. The findings emphasize the likelihood that most glaciers within northwestern British Columbia underwent substantial size and mass balance changes over the last 4000 years, and often spent hundreds of years in advanced positions before retreating.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Ammonia assimilation by Ruminococcus flavefaciens FD-1
No full textStudies were conducted to better understand the mechanism by which Ruminococcus flavefaciens FD-1 utilizes ammonia (-um). \sp{14}C-methyl-ammonium was not taken up by R. flavefaciens FD-1, but cells prepared similarly depleted ammonia from media (initial rates of 11.2 and 0.8 nmolmin\sp{-1}{\cdot}mg\sp{-1} protein for cells grown with limiting nitrogen and carbon, respectively).Glutamine synthetase activity was only detected using the -glutamyl transferase assay. The specific activity was 10-fold higher when cells were grown in ammonia-limiting compared to carbon-limiting defined medium (574 versus 60 nmolmin\sp{-1}{\cdot}mg\sp{-1} for whole cell assays). Specific activity was reduced by 10-30% by sudden exposure of cells grown with ammonia-limitation to high levels of ammonia, but this loss of activity was not consistent with the adenylylation model of post-translational regulation found in the enteric bacteria.Glutamate dehydrogenase activity was 3-fold higher in cells grown in ammonia-limiting compared to carbon-limiting defined medium. Glutamate dehydrogenase was purified 119-fold from batch grown cells. The K\sb{\rm m}'s for ammonia, -ketoglutarate, and glutamate were 19.2, 0.41 and 62 mM, respectively. The sigmoidal NADPH saturation curve revealed positive cooperativity for the binding of this coenzyme. The native enzyme and subunits are 280 and 48 kDa, respectively, suggesting that the native enzyme is a hexamer. The first residue in the amino-terminal amino acid sequence from R. flavefaciens GDH was alanine, suggesting that the protein may be modified post-translationally. Comparison of the amino-terminal sequence with those of E. coli, S. typhimurium and Clostridium symbiosum revealed only 39% amino acid homologies.A degenerate oligonucleotide probe was synthesized and used to screen a recombinant subgenomic library of R. flavefaciens FD-1 chromosomal DNA for the gene encoding glutamate dehydrogenase.Ammonium uptake rates were 1-2 orders of magnitude lower than GS GT specific activity, and 2-3 orders of magnitude lower than that of GDH. This suggests that the enzymatic incorporation of ammonia into amino acid form (or at least glutamate) is not a limiting step in ammonia assimilation.Made available in DSpace on 2011-05-07T13:31:45Z (GMT). No. of bitstreams: 2
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A variety of strategies and funding approaches are required to accelerate the transition to open access. But in all, authors are key
No full textMore than two decades of work towards liberating scholarly publishing from paywalled constraints has left many within the scholarly community exploring ways to accelerate the transition to open access. Not all institutions or author communities will agree upon which strategies or funding approaches to undertake, and nor do they need to. But whichever strategy is pursued, having university faculty lead the charge represents the most effective way forward. Rachael G. Samberg, Richard A. Schneider, Ivy Anderson and Jeff MacKie-Mason share the University of California’s range of open access policy and advocacy materials, and highlight some potential next steps that may be of use to faculty and author communities
Northern Alberta Baseball League Executive
No full textPhotograph - Northern Alberta Baseball League Executive, Athabasca, Alberta. Left to right: Jack Mackie, Jeff Edwards, B.W. Bellamy. Aug 23, 194
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