75 research outputs found
Identification of a Plum pox virus CI-interacting protein from chloroplast that has a negative effect in virus infection
No full textThe cylindrical inclusion (CI) protein of potyviruses is involved in virus replication and cell-to-cell movement. These two processes should rely on multiple plant-virus interactions; however, little is known about the host factors that are involved in, or that may interfere with, CI functions. By using a yeast two-hybrid system, the CI protein from Plum pox virus (PPV) was found to interact with the photosystem I PSI-K protein, the product of the gene psaK, of Nicotiana benthamiana. Coexpression of PPV CI was shown to cause a decrease in the accumulation level of PSI-K transiently expressed in N. benthamiana leaves. To test the biological relevance of this interaction, we have analyzed the infection of PPV in N. benthamiana plants in which psaK gene expression has been silenced by RNA interference, as well as in Arabidopsis thaliana psaK knockout plants. Our results show that downregulation of the psaK gene leads to higher PPV accumulation, suggesting a role for the CI-PSI-K interaction in PPV infection.This work was supported by grants BIO2004-02687 from Spanish MEC and BIO4-CT97-2300 and QLG2-CT-2002-01673 from European Union. I. Jiménez and L. López were recipients of FPI fellowships from the Comu-nidad de Madrid and Spanish MEC, respectively; A. Valli was the recipient of an I3P fellowship from CSIC-Fondo Social Europeo; and J. M. Alamillo held a postdoctoral contract from the Comunidad de Madrid
CRISPR/Cas9 editing of two adenine phosphoribosyl transferase coding genes reveals the functional specialization of adenine salvage proteins in common bean
No full textAdenine metabolism is important for common bean (Phaseolus vulgaris L.) productivity since this legume uses ureides derived from the oxidation of purine nucleotides as its primary nitrogen storage molecules. Purine nucleotides are produced from de novo synthesis or through salvage pathways. Adenine phosphoribosyl transferase (APRT) is the enzyme dedicated to adenine nucleobase salvage for nucleotide synthesis, but it can also convert active cytokinin bases into their inactive nucleotide forms. In common bean, APRT is encoded by four genes. Gene expression analysis, biochemical properties, and subcellular location indicated functional differences among the common bean APRT isoforms. CRISPR/Cas9 targeted down-regulation of two of the four PvAPRTs followed by metabolomic and physiological analyses of targeted hairy roots revealed that, although the two proteins have redundant functions, PvAPRT1 mostly participated in the salvage of adenine, whereas PvAPRT5 was the predominant form in the regulation of cytokinin homeostasis and stress responses with a high impact in root and nodule growth.This work was supported by Grants PID2020-117966RB-100. Ref. /AEI/10.13039/501100011033 (Ministerio de Ciencia e Innovación, Spain), 1380769-R and P20_00440 (Consejería de Transformación Económica, Industria, Conocimiento y Universidades, Junta de Andalucía), BIO-115 (UCO Programa Propio) and Donacion_351/20 (Fundación Torres Gutiérrez, Spain). ARF and SA are supported by the European Union’s Horizon 2020 research and innovation program, under PlantaSYST (SGA-CSA no. 739582 under FPA no. 664620) and project INCREASE (GA 862862). CMLV was supported by fellowships from UCO Programa Propio and EMBO Scientific Exchange Grant. FJMR was supported by Deutsche Forschungsgemeinschaft Project FE552/39-1and by a ‘Margarita Salas’ post-doctoral fellowship (UCOR02MS) from the University of Córdoba (Requalification of the Spanish university system) from the Ministry of Universities financed by the European Union (NexGenerationEU). Funding for open access was provided from Universidad de Córdoba/CBUA.Peer reviewe
Groups with triangle-free graphs on p-regular classes
No full text[EN] Let p be a prime. In this paper, we classify the p-structure of those finite p-separable groups such that, given any three non-central conjugacy classes of p-regular elements, two of them necessarily have coprime lengths.The first and last authors are supported by Ayuda a Primeros Proyectos de Investigación (PAID-06-23) from Vicerrectorado de Investigación de la Universitat Politècnica de València (UPV), and by Proyecto CIAICO/2021/163 from
Generalitat Valenciana (Spain). The results in this paper are part of the second author s Ph.D. thesis.Felipe Román, María Josefa;Jean-Philippe, M.;Sotomayor, V. (2025). Groups with triangle-free graphs on p-regular classes. Mathematische Nachrichten. 298(6):1796-1807. https://doi.org/10.1002/mana.202400554S17961807298
Effects of desiccation on photosynthesis pigments and the ELIP-like desp 22 protein complexes in the resurrection plant Craterostigma plantagineum.
No full textEffects of urate, a natural inhibitor of peroxynitrite-mediated toxicity, in the response of Arabidopsis thaliana to the bacterial pathogen Pseudomonas syringae
No full textUrate, a natural peroxynitrite scavenger, has been used to investígate the possible role of peroxynitrite during plant-pathogen interactions. Urate greatly reduced lesión formation in Arabidopsis leaves treated with an abiotic peroxynitrite-generating system or with a peroxynitrite solution, indicating that it can act as an effective scavenger in planta. In the interaction with the avirulent Pseudomonas syringae pv. phaseolicola [avrRPNW], cell death in the inoculated área was strongly reduced by urate, without compromising disease resistance. In contrast, urate promoted discrete cell death in response to an isogenic Pseudomonas syringae (awRPMT), which did not trigger an HR when inoculated alone, and it induced resistance and arrest of pathogen growth. Scavenging of peroxynitrite did not modify the response of Arabidopsis to an avirulent strain of Xanthomonas campestris pv campestris, that showed a high resistance to NO and peroxynitrite. Our data indícate that peroxynitrite plays a significant role in the responses of plants to Pseudomonas syrin
The source of nitrogen conditions transcriptomic responses to water deficit in common bean roots
No full textDrought stress reduces plant growth and yield of crops. Common bean (Phaseolus vulgaris L.) establishes symbiosis with rhizobia, ensuring an adequate nitrogen supply without fertilizers. However, the relationship with rhizobia is constrained by limited water availability which inhibits both nitrogen fixation and plant growth. In addition, physiological and molecular responses of common bean to drought are conditioned by the form of nitrogen assimilated. Therefore, understanding the molecular mechanism(s) triggered in common bean under water-deficit conditions is relevant to identify the best strategies to resist drought stress. With the objective of understanding the molecular responses of roots and nodules from common bean to water-deficit stress, plants cultivated under N2-fixation or nitrate fertilization were exposed to ten days of water deprivation. Afterwards, transcriptomic analysis was performed in roots, while metabolome profiling was carried out in roots and nodules. Physiological results showed that under water-deficit, N2-fixing plants increased their root biomass more than nitrate-fertilized plants. Furthermore, water-deficit stress induced more transcriptional changes in nitrate-fertilized plants than in N2-fixing plants, including a larger number of transcription factors in these plants compared with the N2-fixing plants. On the other hand, roots from N2-fixing plants accumulated more metabolites with potential protective functions such as allantoin, proline, raffinose, abscisic acid, and flavonoids in response to water-deficit stress than plants fertilized with nitrate, indicating that symbiosis might facilitate a faster and more efficient response to water-deficit stress. Moreover, common bean nodules exposed to water-deficit stress accumulated proline and erythritol, but reduced their content of maltose, pyruvic acid and allantoin compared to their respective controls. Taken collectively, these findings suggest that, despite the inhibition of nodule activity, N2-fixing plants respond better to water-deficit stress than nitrate-fertilized plants.This work was supported by grants PID2020-117966RB-I00 (Ministerio de Ciencia e Innovación, Spain), 1380769-R and P20_00440 (Consejería de Transformación Económica, Industria, Conocimiento y Universidades), BIO-115 (UCO Programa Propio), and Donacion_351/20 (Fundación Torres Gutiérrez, Spain). FT was supported by the European Union—Next Generation EU program and UCOPROYECTA (UCO Programa Propio). CMLV was supported by fellowships from a UCO Programa Propio and EMBO Scientific Exchange grant. FJMR was supported by Deutsche Forschungsgemeinschaft Project FE552/39-1. ARF and SA are supported by the European Union's Horizon 2020 research and innovation program, under PlantaSYST (SGA-CSA no. 739582 under FPA no. 664620) and project INCREASE (GA 862862).Peer reviewe
Editorial: Plant systems biology: integration of system-wide studies to elucidate central features in biological processes
No full textPeer reviewe
Some Properties of Normal Subgroups Determined from Character Tables
No full text[EN] G-character tables of a finite group G were defined in Felipe et al. (Quaest Math, 2022. https://doi.org/10.2989/16073606/16073606.2022.2040633). These tables can be very useful to obtain certain structural information of a normal subgroup from the character table of G. We analyze certain structural properties of normal subgroups which can be determined using their G-character tables. For instance, we prove an extension of the Thompson's theorem from minimal G-invariant characters of a normal subgroup. We also obtain a variation of Taketa's theorem for hypercentral normal subgroups considering their minimal G-invariant characters. This generalization allows us to introduce a new class of nilpotent groups, the class of nMI-groups, whose members verify that its nilpotency class is bounded by the number of irreducible character degrees of the group.The first author is supported by a grant from IPM (No. 1402200112) and the second author is supported by Proyecto CIAICO/2021/163, Generalitat Valenciana (Spain).Akhlaghi, Z.;Felipe Román, María Josefa;Jean-Philippe, M. (2024). Some Properties of Normal Subgroups Determined from Character Tables. Bulletin of the Malaysian Mathematical Sciences Society. 47(3). https://doi.org/10.1007/s40840-024-01684-6S47
Processing of Thionin Precursors in Barley Leaves by a Vacuolar Proteinase
No full textThionins are synthesized as precursors with a signal peptide and a long C-terminal acidic peptide that is post-translationally processed. A fusion protein including the maltose-binding protein from Eschrrichia coli (MalE), thionin DG3 froin barley leaves, and its acidic C-terminal peptide has been used to obtain antibodies that recognize both domains of the precursor. In barley leaf sections. mature thionins accuinulated in the vacuolar content, while the acidic peptide was not detected in any cell fraction. Brefeldin A and inonensin inhibited processing of the precursor but its export from the microsomal fraction was not inhibited. Both purified vacuoles aiid an acid (pH 5.5) extract from leaves processed the fusion protein into a MalE-thionin and an acidic peptide fragment. A 70-kDa proteinase that effected this cleavage was purified froin the acid extract. Processing of the fusion protein by both lysed vacuoles and the purified proteinase was inhibited by Zn2+ and by Cu2+, but not by inhibitors of the previously described vacuolar processing thiol or aspartic proteinases. In vivo processing of the thionin precursor in leaf sections was also inhibited by Zn+, and Cu2+, Variants of the fusion protein with altered processing sites that represented thme of thionin precursors from different taxa were readily processed by the proteinase, whereas changing the polarity of either the C-terminal or N-terminal residues of the processing site prevented cleavage by the proteinase
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