6,718 research outputs found

    Four and a half LIM protein 1C (FHL1C)

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    Four-and-a-half LIM domain protein 1 isoform A (FHL1A) is predominantly expressed in skeletal and cardiac muscle. Mutations in the FHL1 gene are causative for several types of hereditary myopathies including X-linked myopathy with postural muscle atrophy (XMPMA). We here studied myoblasts from XMPMA patients. We found that functional FHL1A protein is completely absent in patient myoblasts. In parallel, expression of FHL1C is either unaffected or increased. Furthermore, a decreased proliferation rate of XMPMA myoblasts compared to controls was observed but an increased number of XMPMA myoblasts was found in the G(0)/G(1) phase. Furthermore, low expression of K(v1.5), a voltage-gated potassium channel known to alter myoblast proliferation during the G(1) phase and to control repolarization of action potential, was detected. In order to substantiate a possible relation between K(v1.5) and FHL1C, a pull-down assay was performed. A physical and direct interaction of both proteins was observed in vitro. In addition, confocal microscopy revealed substantial colocalization of FHL1C and K(v1.5) within atrial cells, supporting a possible interaction between both proteins in vivo. Two-electrode voltage clamp experiments demonstrated that coexpression of K(v1.5) with FHL1C in Xenopus laevis oocytes markedly reduced K(+) currents when compared to oocytes expressing K(v1.5) only. We here present the first evidence on a biological relevance of FHL1C

    H-1 and K-39 spin-lattice relaxation, ionic motion and Raman processes in the proton conducting KHSeO4 single crystal

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    The spin-lattice relaxation rates of H-1 and K-39 nuclei in KHSeO4 crystals were studied in the temperature range 160-400 K. The spin-lattice relaxation recovery of H-1 nucleus in this crystal can be represented with a single exponential function, and the relaxation T-1(-1) curve of H-1 can be represented with the Bloembergen-Purcell-Pound (BPP) function. The relaxation process of K-39 with dominant quadrupole relaxation can be described by a linear combination of two exponential functions. T-1(-1) for the K-39 nucleus was found to have a very strong temperature dependence, T-1(-1) = beta T-7. Rapid variations in relaxation rates are associated with critical fluctuations in the electronic spin system. The T-7 temperature dependence of the Raman relaxation rate is shown here to be due to phonon-magnon coupling. (c) 2005 Elsevier Ltd. All rights reserved

    Yves-Heng Lim

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    Yves-Heng Lim est enseignant-chercheur au Département d’Etudes de Sécurité et de Criminologie de l’Université Macquarie, Sydney. Il est l’auteur de China’s Naval Power: An Offensive Realist Approach (Ashgate, 2014). Yves-Heng Lim is a lecturer at the Department of Security Studies and Criminology, Macquarie University. He is the author of China’s Naval Power: An Offensive Realist Approach (Ashgate, 2014)
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