1,720,980 research outputs found
IMUNOHISTOCHEMTSTRY METHOD TO DETECT C-REACTIVE PROTEIN IN ATHEROMA PLAQUES OF SPRAGUE DAWLEY RATS FED HIGH LIPID RATION
No full textSince Inflammation is believed to have role in pathogenesis of cardiovasculare vents, measurement of markers of inflammation has been proposed as a method to improve the prediction of the risk of these events. C-reactive protein (CRP) a major acute phase protein, has been associated with the presence and severity of atherosclerosis, and has been found to predict acute vasculare vents in prospective studies. The aim of this study was to analyse the relationship between appearance of CRP in aorta and atheroma plaque formation in Sprague Dawley rats after 59 days induction with high fat diet. Ten male Sprague Dawley rats, 1.5 months of age were used as experimental animals. Rats were adapted in l0 single cages for 5 days and given basal diet containing normal fat and water ad libitum. After adaptation, rats were divided in to 2 groups (group 1 and group 2) of 5 each. Group I was fed basal diet containing normal fat(4.5% of fat) and group 2 was fed diet containing high fat (20% of fat). After 59 days, all rats were killed, the heart including aorta were taken out for histophatologic (HE) and immunohistochemistry analyses. The result of this study showed that all rats in Group 1 did not have atheroma plaque and CRP negative, but rats in G"cup 2, all have atheroma plaque (100%) but only 60% CRP positive. From those result it concluded that although all aortas have atheroma plaque, the CRP may not be detected in the plaque.Keywords: atheroma plaques, inflammation, C-reactive protein
Penggunaan Ventilator dalam Menunjang Keberhasilan Operasi Hernia Diafragmatika karena Trauma Pada Kucing Lokal
No full textVentilator merupakan perangkat vital pada mesin anestesi inhalasi yang sangat diperlukan untuk penanganan hernia diafragmatika, yang banyak ditemukan kasusnya pada kucing, di Daerah Istimewa Yogyakarta. Mayoritas kasus hernia diafragmatika disebabkan akibat trauma, baik karena jatuh dari ketinggian, rudapaksa maupun tertabrak kendaraan bermotor. Operasi penanganan hernia diafragma tergolong sulit dikarenakan hilangnya kemampuan ventilasi akibat sobeknya diafragma akan berakibat kolapsnya paru paru yang rawan menyebabkan kematian. Penggunaan mesin ventilator akan sangat membantu dalam proses operasi, karena ventilasi mekanik akan mempertahankan suplai oksigen secara stabil sampai dengan operasi selesai dilaksanakan. Monitoring stabilitas kondisi pasien sebelum, selama dan sesudah operasi merupakan prosedur yang tidak kalah pentingnya dalam penanganan hernia diafragmatika. Hewan biasanya akan bernafas normal dan menunjukkan perbaikan kondisi dalam beberapa hari setelah operasi selesai
Hypertropic Osteopathy (Marie Diseases) in The German Shepherd Dog
No full textAbstract A Male German Shepherd dog, five years old, twenty six kg of body weight was submitted to Professor Soeparwi Animal Hospital, Faculty of Veterinary Medicine, Gadjah Mada University by Mr. Siswo Han, the owner who lives in Cangkringan, Yogyakarta on May 13, 2013. The dog was suffered from the pain and lameness on the front-left extremity for about 2 weeks. The dog having registered number of 8738/V/A/RSH/13 did not show any specific clinical signs, except the lameness. Skin and hairs, mucous membranes, lymphoid glands, and respiratory, digestive, circulatory and uropoetica organs as well were within the normal limits. The result of standard blood test for both red blood cell and white blood cell were still within normal values. Evaluation of fecal sample of the dog was also normal meaning that no parasites were found in the fecal sample. Radiographic analysis of the upper left extremity and the lungs area showed that there was an irregular bone growth on the left caput humeri, and the lungs were normal. Based on the lesion, the dog was diagnosed as having a hyperthropic osteopathy (Marie Disease).
Hemangiosarcoma in The Golden Retriever
No full textA Golden retriever dog, male, 9 years old was a patient of Surgery Clinic, Department of Surgery and Radiology, Faculty of Veterinary Medicine, Gadjah Mada University. The patient was dated on September 27, 2012. Clinically observation showed skinny, normal appetite, and not showing any physiologic change except that there were superficially bumps on the inguinal skin 4 cm in diameter size. The tumor biopsy was performed using lidocaine 2% as local anaesthetic circullary application. Histopathologically analysis of the tumor tissue was identified as hemangiosarcoma
HUBUNGAN KONSENTRASI MALONDIALDEHIDA, GLUKOSA DAN TOTAL KOLESTEROL PADA TIKUS PUTIH YANG DIINJEKSI DENGAN STREPTOZOTOCIN
No full textDiabetes mellitus is a major source of morbidity in developed countries. The morbidity and mortality areincreased in patients with diabetes mellitus compared with the general population. Relationship between highsugar or fat consumption with the increasing of glucose or blood cholesterol concentration has been wellunderstood, but the mechanism of diabetes mellitus as a result of streptozotocin injection and its relation withglucose and cholesterol concentration still need to be investigated. So far, many research mention thatstreptozotocin causes only the damage of a cell of pancreas which that directly relates to metabolism of bloodsugar. The purpose of the research is to investigate the mechanism of pancreas damage in its relation with lipidperoxidation. Sixteen male Wistar rats, 200 grams of bodyweight were used as animal models. Rats weredivided into two groups of eight animals each. Group I was not injected with streptozotocin, while Group II isinjected intraperitoneally with streptozotocin 50 mg/kg BW.Three days after the injection, blood samples weretaken intracardially for Malondialdehida (MDA), glucose and total cholesterol analysis. A one-way analysis ofvariance showed that there were significance differences between Group I and II (
THE EFFECT OF HIGH LIPID DIET LIPOPOLYSACCHARIDE AND LAPARATOMY SURGERY ON THE LEVEL OF C-REACTIVE PROTEIN SERUM IN SPRAGUE DAWLEY RATS EKD
Full text linkC-reactive protein (CRP) is a member of the family of proteins known as pentraxins, and originally defined as a substance, observed in the serum or plasma of patient with acute infections, that reacted with the Cpolysaccharide of the pneumococcus. Serum concentrations of CRP increase as part of the inflammatory response to infection or acute injury. The aim of the study was to see the differences between acute and chronicstimuli in the increasing of CRP concentration in serum of Sprague Dawley rat. Twenty male Sprague Dawley rats, 1.5 months of age were used as experimental animals. Rats were adapted for 5 days and given basal diet,containing normal fat(5% of fat) and water ad libitum. Rats were then divided into 4 groups of 5 each. Group 1 was used as control animal. The animals in this group were fed basal diet for 59 days without any othertreatments. Group 2 was animals fed high fat diet containin g20Yo of fat, for 59 days without any other treatments. Group 3 was animals fed basal diet for 59 days and in the day of 60 then injected with LPS from Escherichia coli 0111:B4, 1 mg/kg BW intraperitoneally, and Group 4 was animals fed basal diet for 59 days and in the day of 60 animals then laparatomized. Twenty four hours after all treatments, blood sample were collected from orbitally vein, serum was separated and used to analyze CRP concentration. Statistical analyzes using onew ay analyzeso fvariances howed that there were significance differences among Group 1,2,3 and 4 (p<0.05). Mean of CRP concentrations respectively from the highest to the lowest were: Group 3, Group 4, Group 2 and Group 1. Fromthe result of the study it can be concluded that CRP concentration of acute stimulus was higher than chronic stimulus.Key words : C-reactivep rotein, lipopolysaccharides, high fat diet, laparatomy
The Analysis of Total Leucocyte, C-Reactive Protein and Fibrinogen Concentrations to Evaluate The Leakage of Enterectomy Result
Full text linkEnterectomy is an operative method that has very dangerous risk. The purpose of this research was done to see the physiological view after surgery, especially in the total leucocyte, C-reactive protein (CRP) andfibrinogen concentrations. Nine female, healthy local dogs, 10 kgs of body weight were used as experimental study. The dogs were adapted for a week into individual cages and fed commercial dog-food and water adlibitum. In the day of 7, all dogs were fasted 12 hours for anaesthesia preparation. The dogs were then divided into 3 Groups of 3 each. Group I was used as control, it had normal enterectomy surgery. Group II was animals that had enterectomy surgery with one hole leakage. Group III was animals that had enterectomy surgery with two special hole leakages. Enterectomy itself was done on to jejunum with 5 cm of gut should be cut away. Anastomosis method was done using end to end method and interrupted suturing method with chromic cat gut 0/3. The result of the research showed that there is similarly increasing level of leucocyte and CRP in the second day after surgery and decreased gradually until the sixth day, but different type of changes in fibrinogen level. The increasing level of CRP has advantages, such as it can be used as eidker marker of inflammation or infection, or worse condition after enterectomy.Keywords : enterectomy, leucocyte, C-reactive protein, fibrinogen, inflammatio
HUBUNGAN KONSENTRASI MALONDIALDEHIDA, GLUKOSA DAN TOTAL KOLESTEROL PADA TIKUS PUTIH YANG DIINJEKSI DENGAN STREPTOZOTOCIN
No full textDiabetes mellitus is a major source of morbidity in developed countries. The morbidity and mortality areincreased in patients with diabetes mellitus compared with the general population. Relationship between highsugar or fat consumption with the increasing of glucose or blood cholesterol concentration has been wellunderstood, but the mechanism of diabetes mellitus as a result of streptozotocin injection and its relation withglucose and cholesterol concentration still need to be investigated. So far, many research mention thatstreptozotocin causes only the damage of a cell of pancreas which that directly relates to metabolism of bloodsugar. The purpose of the research is to investigate the mechanism of pancreas damage in its relation with lipidperoxidation. Sixteen male Wistar rats, 200 grams of bodyweight were used as animal models. Rats weredivided into two groups of eight animals each. Group I was not injected with streptozotocin, while Group II isinjected intraperitoneally with streptozotocin 50 mg/kg BW.Three days after the injection, blood samples weretaken intracardially for Malondialdehida (MDA), glucose and total cholesterol analysis. A one-way analysis ofvariance showed that there were significance differences between Group I and II (
IMUNOHISTOCHEMTSTRY METHOD TO DETECT C-REACTIVE PROTEIN IN ATHEROMA PLAQUES OF SPRAGUE DAWLEY RATS FED HIGH LIPID RATION
Full text linkSince Inflammation is believed to have role in pathogenesis of cardiovasculare vents, measurement of markers of inflammation has been proposed as a method to improve the prediction of the risk of these events. C-reactive protein (CRP) a major acute phase protein, has been associated with the presence and severity of atherosclerosis, and has been found to predict acute vasculare vents in prospective studies. The aim of this study was to analyse the relationship between appearance of CRP in aorta and atheroma plaque formation in Sprague Dawley rats after 59 days induction with high fat diet. Ten male Sprague Dawley rats, 1.5 months of age were used as experimental animals. Rats were adapted in l0 single cages for 5 days and given basal diet containing normal fat and water ad libitum. After adaptation, rats were divided in to 2 groups (group 1 and group 2) of 5 each. Group I was fed basal diet containing normal fat(4.5% of fat) and group 2 was fed diet containing high fat (20% of fat). After 59 days, all rats were killed, the heart including aorta were taken out for histophatologic (HE) and immunohistochemistry analyses. The result of this study showed that all rats in Group 1 did not have atheroma plaque and CRP negative, but rats in G"cup 2, all have atheroma plaque (100%) but only 60% CRP positive. From those result it concluded that although all aortas have atheroma plaque, the CRP may not be detected in the plaque.Keywords: atheroma plaques, inflammation, C-reactive protein
- …
