13 research outputs found
Synthesis of quinoxaline derivatives catalyzed by Brønsted acidic ionic liquid under solvent-free conditions
No full textBrønsted acidic ionic liquid (BAIL) found an efficient and eco-friendly for quinoxaline preparation via condensation of 1,2-phenylene diamines and 1,2-diketones. This simple protocol is carried out under solvent-free condition and at room temperature. Relatively short reaction times, high yields, efficiency, generality, simple methodology, low-cost materials, easy work-up, ease of preparation and regeneration of the catalyst and green conditions are the main advantages of this protocol. © 2020 Author(s).A. Majee acknowledges financial support from the CSIR-major research project (ref. No. 02(0383)/19/EMR-II). A. Mukherjee is thankful to the Russian Science Foundation – Russia (ref. # 18-73-00301) for funding. We are thankful to the DST-FIST and UGC-SAP program
Artificial Seed Production of Tylophora indica for Interim Storing and Swapping of Germplasm
No full textOur research work demonstrates the single bead alginate-encapsulation, interim storing and conversion of Tylophora indica (Burm. Fil.) Merrill. Most effective encapsulation of in vitro nodal segments [(4 ± 1) mm long], ensuing in sphere-shaped artificial seeds of similar morphology, was achieved through 75 mmol⋅L−1 calcium chloride (CaCl2 ⋅ 2H2O) plus 3% (w/v) Na-alginate with 93.3% conversion frequency. The earliest conversion (within 7 days of incubation) of artificial seeds occurred in half-strength liquid Murashige and Skoog medium. Among the three different temperature regimes [(5 ± 1) °C, (15 ± 1) °C, and (25 ± 1) °C], storage of artificial seeds at (15 ± 1) °C executed the highest frequency of conversion (90%) after 15 days of storage. Lengthier storage significantly reduced the conversion frequency of artificial seeds irrespective of storage temperature. Nevertheless, the conversion frequency after 30 days of storage at (15 ± 1) °C was recorded at 70% without further decline even following 45 days of storage, which evidently suggests that lower temperature (15 ± 1) °C is apt for storage and subsequent conversion of T. indica artificial seeds. The present protocol could be expedient for short-term storing and swapping of T. indica germplasms between national and international laboratories
ABI5 binding proteins : key players in coordinating plant growth and development
No full textDuring the course of terrestrial evolution, plants have developed complex networks that involve the coordination of phytohormone signalling pathways in order to adapt to an ever-changing environment. Transcription factors coordinate these responses by engaging in different protein complexes and exerting both positive and negative effects. ABA INSENSITIVE 5 (ABI5) binding proteins (AFPs), which are closely related to NOVEL INTERACTOR OF JAZ (NINJA)-like proteins, are known for their fundamental role in plants' morphological and physiological growth. Recent studies have shown that AFPs regulate several hormone-signalling pathways, including abscisic acid (ABA) and gibberellic acid (GA). Here, we review the genetic control of AFPs and their crosstalk with plant hormone signalling, and discuss the contributions of AFPs to plants’ growth and development
Differentially expressed myo-inositol monophosphatase gene (CaIMP) in chickpea (Cicer arietinum L.) encodes a lithium-sensitive phosphatase enzyme with broad substrate specificity and improves seed germination and seedling growth under abiotic stresses
No full textmyo-Inositol monophosphatase (IMP) is an essential enzyme in the myo-inositol metabolic pathway where it primarily dephosphorylates myo-inositol 1-phosphate to maintain the cellular inositol pool which is important for many metabolic and signalling pathways in plants. The stress-induced increased accumulation of inositol has been reported in a few plants including chickpea; however, the role and regulation of IMP is not well defined in response to stress. In this work, it has been shown that IMP activity is distributed in all organs in chickpea and was noticeably enhanced during environmental stresses. Subsequently, using degenerate oligonucleotides and RACE strategy, a full-length IMP cDNA (CaIMP) was cloned and sequenced. Biochemical study revealed that CaIMP encodes a lithium-sensitive phosphatase enzyme with broad substrate specificity, although maximum activity was observed with the myo-inositol 1-phosphate and l-galactose 1-phosphate substrates. Transcript analysis revealed that CaIMP is differentially expressed and regulated in different organs, stresses and phytohormones. Complementation analysis in Arabidopsis further confirmed the role of CaIMP in l-galactose 1-phosphate and myo-inositol 1-phosphate hydrolysis and its participation in myo-inositol and ascorbate biosynthesis. Moreover, Arabidopsis transgenic plants over-expressing CaIMP exhibited improved tolerance to stress during seed germination and seedling growth, while the VTC4/IMP loss-of-function mutants exhibited sensitivity to stress. Collectively, CaIMP links various metabolic pathways and plays an important role in improving seed germination and seedling growth, particularly under stressful environments. © The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology
TRIPOD TYPE 2,2′-BIPYRIDINE LIGAND FOR THE LANTHANIDE CATIONS: THE SYNTHESIS AND THE PHOTOPHYSICAL STUDIES ON COORDINATION TO TRANSITION METAL CATIONS
No full textNew tripod-type 2,2'-bipyridine ligand consisting of a central polyaminocarboxylic moiety for the coordination to lanthanide cations and three appended 5-phenyl-2,2'-bipyridine fragments for the coordination to various transition metal cations have been prepared. A europium complex of this ligand was prepared, and its photophysical properties and a luminescent response towards transition metal salts (particularly, CdI2, Cd(OAc)2, Zn(ClO4)2, Cu(OAc)2, HgCl2) have been studied. Europium cation luminescence quenching in the presence of transition metal salts in solution was observed in all cases. In addition, it was observed that the fluorescent response of the europium complex was quite individual depending on the type of the metal salt. The obtained data were compared with the earlier published data for some lanthanide complexes bearing additional sites for the chelation of transition metal cations.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Flexible organic–inorganic hybrid layer encapsulation for organic opto-electronic devices
No full textIn this work we produce and study the flexible organic–inorganic hybrid moisture barrier layers for the protection of air sensitive organic opto-electronic devices. The inorganic amorphous silicon nitride layer (SiNx:H) and the organic PMMA [poly (methyl methacrylate)] layer are deposited alternatingly by using hot wire chemical vapor deposition (HW-CVD) and spin-coating techniques, respectively. The effect of organic–inorganic hybrid interfaces is analyzed for increasing number of interfaces. We produce highly transparent (∼80% in the visible region) hybrid structures. The morphological properties are analysed providing a good basis for understanding the variation of the water vapor transmission rate (WVTR) values. A minimum WVTR of 4.5 × 10−5g/m2day is reported at the ambient atmospheric conditions for 7 organic/inorganic interfaces. The hybrid barriers show superb mechanical flexibility which confirms their high potential for flexible applications.The authors would like to thank Dr. J.C. Vanel for help in electrical characterizations used in this study. The first author (S.M) acknowledges the financial support from Direction des Relations Extérieures, Ecole Polytechnique during his thesis
Unsymmetrically functionalized 5,5''-diaryl- and 5,6,5''-triaryl-2,2':6',2''-terpyridines: an efficient synthetic route and photophysical properties
No full textAn efficient approach for the synthesis of 5,5''- or 5,6,5''-arylsubstituted 2,2':6',2''-terpyridines, bearing an anneleted cyclopentene unit in one of the side-chain pyridine ring for the improved solubility in organic solvents, via their 1,2,4-triazine analogues has been developed. By using this approach, various aromatic substituents were introduced in the 2,2':6',2''-terpyridine core. Depending on the nature of the aromatic substituents the obtained terpyridines exhibited an intense emission in a range of ca. 344â 394 nm in acetonitrile solutions. For the most representative compounds a pronounced bathochromic shifts in both absorption and emission spectra were observed compare to previously reported substituted terpyridines.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Cellular Responses in the Pigeonpea Wild Relative Cajanus platycarpus to Helicoverpa armigera Herbivory: The Role of Methionine Sulfoxide Reductase B1 (CpMSRB1) in Enhanced Defense
No full textUnderstanding key cellular mechanisms leading to improved defense against various stressors is essential for cultivating robust nutritious crops capable of flourishing in diverse environments. We present an in-depth characterization of the defense response in the pigeonpea wild relative Cajanus platycarpus to herbivory by pod borer Helicoverpa armigera. To fight the attacking pest, C. platycarpus strategically activated non-enzymatic reactive oxygen species (ROS) scavengers and unleashed methionine sulfoxide reductases to safeguard the integrity of methionine residues. We unveiled for the first time physical interaction between CpMSRB1 and chorismate mutase (CpCM1.1), a pivotal player in the phenylpropanoid pathway. This association fueled the synthesis of phenylpropanoids and enhanced ROS scavenging crucial for repelling herbivores. Repairing CpCM1.1 also boosted salicylic acid production, coordinating defense signaling with jasmonic acid. Additionally, heterologous expression of CpMSRB1 in tomato improved defense against herbivory by enhanced ROS scavenging and polyphenol production. This study demonstrates the role of CpMSRB1 in protecting a major enzyme in the shikimate pathway, reinforcing defense against H. armigera. [Figure: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license
