2,264 research outputs found
Nuclear translocation and signalling of L1-CAM in human carcinoma cells requires ADAM10 and presenilin/gamma-secretase activity
No full textL1-CAM (L1 cell-adhesion molecule), or more simply L1, plays an important role in the progression of human carcinoma. Overexpression promotes tumour-cell invasion and motility, growth in nude mice and tumour metastasis. It is feasible that L1-dependent signalling contributes to these effects. However, little is known about its mechanism in tumour cells. We reported previously that L1 is cleaved by ADAM (a disintegrin and metalloprotease) and that the cytoplasmic part is essential for L1 function. Here we analysed more closely the role of proteolytic cleavage in L1-mediated nuclear signalling. Using OVMz carcinoma cells and L1-transfected cells as a model, we found that ADAM10-mediated cleavage of L1 proceeds in lipid raft and non-raft domains. The cleavage product, L1-32, is further processed by PS (presenilin)/gamma-secretase to release L1-ICD, an L1 intracellular domain of 28 kDa. Overexpression of dominantnegative PS1 or use of a specific gamma-secretase inhibitor leads to an accumulation of L1-32. Fluorescence and biochemical analysis revealed a nuclear localization for L1-ICD. Moreover, inhibition of ADAM10 and/or gamma-secretase blocks nuclear translocation of L1-ICD and L1-dependent gene regulation. Overexpression of recombinant L1-ICD mediates gene regulation in a similar manner to full-length L1. Our results establish for the first time that regulated proteolytic processing by ADAM10 and PS/gamma-secretase is essential for the nuclear signalling of L1 in human carcinoma cell lines. Key words: a disintegrin and metalloprotease 10 (ADAM10), L1 cell-adhesion molecule (L1-CAM), nuclear translocation, presenilin (PS)/gamma-secretase activity, raft, signalling
Changes in self-reported and parent-reported health-related quality of life in overweight children and adolescents participating in an outpatient training: findings from a 12-month follow-up study
No full textFinne E, Reinehr T, Schaefer A, Winkel K, Kolip P. Changes in self-reported and parent-reported health-related quality of life in overweight children and adolescents participating in an outpatient training: findings from a 12-month follow-up study. Health and Quality of Life Outcomes. 2013;11(1): 1.Background
Health-related quality of life (HRQoL) was found to improve in participants of weight management interventions. However, information on moderately overweight youth as well as on maintaining HRQoL improvements following treatment is sparse. We studied the HRQoL of 74 overweight, but not obese participants (32.4% male, mean age = 11.61 ± 1.70 SD) of a comprehensive and effective six-month outpatient training at four time-points up to 12 months after end of treatment.
Methods
HRQoL was measured by self-report and proxy-report versions of the generic German KINDL-R, including six sub domains, and an obesity-specific additional module. Changes in original and z-standardized scores were analyzed by (2×4) doubly multivariate analysis of variance. This was done separately for self- and proxy-reported HRQoL, taking into account further socio-demographic background variables and social desirability. Additionally, correlations between changes in HRQoL scores and changes in zBMI were examined.
Results
There were significant multivariate time effects for self-reported and proxy-reported HRQoL and a significant time-gender interaction in self-reports revealed (p < .05). Improvements in weight-specific HRQoL were evident during treatment (partial η2 = 0.14-0.19). Generic HRQoL further increased after end of treatment. The largest effects were found on the dimension self-esteem (partial η2 = 0.08-0.09 for proxy- and self-reported z-scores, respectively). Correlations with changes in weight were gender-specific, and weight reduction was only associated with HRQoL improvements in girls.
Conclusions
Positive effects of outpatient training on generic and weight-specific HRQoL of moderately overweight (not obese) children and adolescents could be demonstrated. Improvements in HRQoL were not consistently bound to weight reduction. While changes in weight-specific HRQoL were more immediate, generic HRQoL further increased after treatment ended. An extended follow-up may therefore be needed to scrutinize HRQoL improvements due to weight management
Fishing Power Functions in Aggregate Bioeconomic Models
No full textA method for estimating fishing power in the Beverton-Holt tradition in the absence of firm-level data is developed. This enables the construction of a standardized measure of fishing effort that can facilitate the analysis and implementation of various management alternatives. The methodology is applied to the Gulf of Mexico Reef Fish Fishery.Environmental Economics and Policy, Research Methods/ Statistical Methods, Resource /Energy Economics and Policy, Risk and Uncertainty,
Enacting Community on Dartmoor: MED Theatre’s Badgerland: A Dartmoor Comedy (2015) and the spatial praxis of community theatre
No full textThis is the author accepted manuscript. The final version is available from Palgrave Macmillan via the DOI in this recordArts and Humanities Research Council (AHRC
Optical spectroscopy of the low-mass X-ray binary GX 9+9
No full textPhase-resolved medium-resolution VLT spectroscopy of the low-mass X-ray binary GX 9+9 has revealed narrow C ill emission lines that move in phase relative to our new estimate of the ephemeris, and show a velocity amplitude of 230 +/not superset of 35 km s(-1). We identify the origin of these lines as coming from the surface of the donor star, thereby providing the first estimate of the mass function of f(M-1) >= 0.22 M-circle dot. Rotational broadening estimates together with assumptions for the mass donor give 0.07 <= q <= 0.35 and 182 <= K-2 <= 406 km s(-1). Despite a low-mass ratio, there is no evidence for a superhump in our data set. Doppler maps of GX 9+9 show the presence of a stream overflow, either in the form of material flowing downward along the accretion disc rim or in a similar fashion as occurs in high mass transfer rate cataclysmic variables known as the SW Sex stars. Finally, we note that the Bowen region in GX 9+9 is dominated by C in instead of N III emission as has been the case for most other X-ray binaries
Matthaei Parker Cantuariensis archiepiscopi De antiquitate Britannicae ecclesiae et privilegiis ecclesiae Cantuariensis : cum archiepiscopis ejusdem LXX e XXI exemplarium 1572 excusorum, sibique mutuo sorte plane singulari discrepantium, collatione, integra nunc primum numerisque absoluta omnibus historia /
No full textIncludes index.Engraved portrait of Parker by George Vertue; engraved head- and tail-pieces, pictorial initials.Signatures: pi² *⁴ A⁸ chi²(-chi2) ²A² B-L²,²chi²(-²chi2) M-Q²,²B-Q² R-8L².Mode of access: Internet.Bookplate of Revd. George Williams. Signature of Wm. H. Ink, 1879. Signature of Peter Karney, Cambridge, July 1939. Signature on t.p. of Schaefer Williams, Bay Settlement, Wis., 8 July 1976.Binding: 19th-century marbled paper, quarter goatskin. Author & title on spine in gilt. Edges sprinkled red & tan
MEASUREMENT OF THE VIBRATION-ROTATION SPECTRUM OF THE HYDROXIDE ANION () BY VELOCITY MODULATION LASER SPECTROSCOPY
No full text. T. J. Lee and H. F. Schaefer III, J. Chem. Phys. 83, 1784 (1985). 2. H.-J. Werner, P. Rosmus, and E.-A. Reinsch, J. Chem. Phys. 79, 905 (1983). 3. P.A. Schulz, R.D. Mead, P. L. Jones, and W.C. Lineberger, J. Chem. Phys. 77, 1153 (1982). 4. N.H. Rosenbaum, J. C. Owrutsky, L. M. Tack, and R.J. Saykally, J. Chem. Phys. (accepted). Address of Rosenbaum, Owrutsky, Tack, and Saykally: Department of Chemistry, University of California, Berkeley, CA 94720.Author Institution:Guided by the ab initio predictions for the fundamental made by Lee and Schaefer (1) and by Werner, Rosmus, and Reinsch (2) along with the laser photodetachment results of Schulz et al. (3), we measured the band of and by velocity modulation spectroscopy with a color center laser (4). The concentration was found to be maximized in a or discharge where the addition of argon both rotationally heats the and increases its overall concentration. The signal was also found to be dramatically dependent on the presence of metal sputtered on the discharge cell wall. The and bands were analyzed separately with a least squares analysis yielding the equilibrium bond length and vibration-rotation constants through sextic distortion terms. Using the band origins of the two isotopomers, the harmonic vibrational frequencies and first order anharmonicities were calculated
Impact of treatment planning target volumen (PTV) size on radiation induced diarrhoea following selenium supplementation in gynecologic radiation oncology - a subgroup analysis of a multicenter, phase III trial
No full textBackground: In a previous analysis (Int J Radiat Oncol Biol Phys 70:828-835,2010), we assessed whether an adjuvant supplementation with selenium (Se) improves Se status and reduces the radiation-induced side-effects of patients treated by adjuvant radiotherapy (RT) for cervical and uterine cancer. Now, a potential relation between the planning target volume (PTV) of the RT and the Se effect concerning radiation induced diarrhoea was evaluated in detail.
Methods: Whole blood Se concentrations had been measured in patients with cervical (n=11) and uterine cancer (n=70) after surgical treatment, during, and at the end of RT. Patients with initial Se concentrations of less than 84 μg/l were categorized as Se-deficient and randomized before RT to receive Se (as sodium selenite) per os on the days of RT, or to receive no supplement during RT. Diarrhoea was graded according to the Common Toxicity Criteria system (CTC, Version 2a). The evaluation of the PTV of the RT was ascertained with the help of a specialised computer-assisted treatment planning software used for radiation planning procedure.
Results: A total of 81 patients had been randomized for the initial supplementation study, 39 of which received Se [selenium group, SeG] and 42 serving as controls [control group, CG]. Mean Se levels did not differ between SeG and CG upon study initiation, but were significantly higher in the SeG compared to the CG at the end of RT. The actuarial incidence of at least CTC 2 radiation induced diarrhoea in the SeG was 20.5% compared to 44.5% in the CG (p=0.04). The median PTV in both groups was 1302 ml (916–4608). With a PTV of 1302 ml (n=40) the actuarial incidence of at least CTC 2 diarrhoea in the SeG was 19.1% (4 of 21 patients) versus 52.6% (10 of 19 patients) in the CG (p=0.046).
Conclusions: Se supplementation during RT was effective to improve blood Se status in Se-deficient cervical and uterine cancer patients, and reduces episodes and severity of RT-induced diarrhoea. This effect was most pronounced and significant in patients with large PTV (> 1302 ml)
Brachycerocoris camelus Costa 1863
No full textBrachycerocoris camelus Costa, 1863 (Fig. 1a, b & Fig. 7. 1a–d) Material examined. CHINA: Fukien: 1♀ [DAR], Chungan, Bohea hill, 9.x.1939, T. C. Maa. Differential diagnosis. Similar to B. petrii sp. nov., separated from the other two specie s B. dromedarius and B. davidii sp. nov. by the absence of the pointed tubercle on lateral mandibular plates. Differs from B. petrii sp. nov. in the presence of a well developed central tubercle on anterior half of pronotum, which is distinctly longer than width at the base of tubercle; the apex of central tubercle on anterior half of pronotum nearly truncate; the central margin connecting base of central tubercle (anteriomedial tubercle) of scutellum with posteromedial tubercle, C-shaped in lateral view; the pygophore with the caudal angles developed into long, stout horn-like processes, divergent each other; the paramere L-shaped, apex of crown as long as broad, round and clubbed at apex with concave depression on innerside and a dense tuft of setae, projected caudad at the base of the depression; stem of uniform width throughout (Schaefer et al. 1996: Figs. 3 C & D). Male genitalia. Genital capsule subquadrangular, caudal angles developed into long, stout horn-like processes, apically slightly divergent from each other. Ventral rim deep, concave, broadly U-shaped. Paramere with single, straight arm, crown broad, slightly L-shaped, round and clubbed at apex. Dense tuft of setae at the innerside of the joint between arm and crown (Schaefer et al. 1996). Remarks. Schaefer et al. (1996) dealt with two species of Brachycerocoris namely B. camelus and B. dromedarius. The second author had an opportunity to examine the single female specimen of B. camelus in David Rider’s laboratory and could clearly associate it with the habitus images and morphological description given by Schaefer et al. (1996) for B. camelus. The female genitalia of B. camelus was not dissected out by considering the comparatively less significance of it in species determination and also to avoid the destruction of the single available specimen; whereas the availability of enough materials of B. dromedarius in the personal collection of the second author [MRC] facilitated the authors to dissect out and study the post abdominal structures of this species besides comparing the external morphology of it with the description given by Schaefer et al. (1996). Based on this study the authors could understand that the figures (Fig. 3.A & B of Schaefer et al. 1996) labelled as B. camelus actually correspond to B. dromedarius and the other (Fig. 3.C & D of Schaefer et al. 1996) would obviously that of B. camelus as only two species were dealt with. Distribution. China!, India?, Sri Lanka? Distributional remarks. The general external morphology of B. camelus and B. petrii Salini and Roca-Cusachs, sp. nov. resemble each other in general external morphology and can lead to erroneous identification unless the fine details of the external morphology and of male genitalia are closely examined. Therefore, the records from India and Sri Lanka probably correspond to the species B. petrii Salini and Roca-Cusachs, sp. nov., though the records need verification. This is because an effort to study the post abdominal structures of the species of Brachycerocoris available from those regions was never attempted. It is also evident from the differences pointed out by Schaefer (1997) among the specimens collected from India and China with respect to the length and height of medial tubercle on pronotum and scutellum and the shape of tubercle between eyes.Published as part of Salini, S. & Roca-Cusachs, M., 2021, Review of the Oriental species of the genus Brachycerocoris Costa, 1863 (Hemiptera: Pentatomoidea: Pentatomidae: Podopinae s. l.) with description of two new species, pp. 507-527 in Zootaxa 5040 (4) on pages 509-510, DOI: 10.11646/zootaxa.5040.4.3, http://zenodo.org/record/553170
Nebraska Transcript (University of Nebraska College of Law), Fall 2022
No full textDean\u27s Message
Remembering Anna Williams Shavers 4
Sold-out Women Lead event empowers leaders 9
Reward outweighs risk at Lavender Legal Center 12
LaChandra Pye, \u2709, named multicultural homecoming nominee 14
Nebraska Legal Diversity Council founded 16
Sullivan recognized for work on Tenant Assistance Project 18
Children’s Justice Attorney Education Program selects inaugural Fellows 20
White House, Attorney General recognize Tenant Assistance Project 22
Faculty Notes 24
Matthew Schaefer named Clayton Yeutter Chair 32
Student team wins regional patent application drafting competition 34
Three faculty earn professorships: Kristen Blankley, Jessica Shoemaker, Adam Thimmesch 35
Housing Justice Program offers array of opportunities 36
Magilton & students contribute paper to European Centre for Excellence 38
Stohs & EClinic recognized at Annual Entrepreneurship Summit 40
Beard: Guiding the Law of Military Space Operations 41
Dean’s Advisory Board honorees recognized 42
Celebrating the Class of 2022 43
AlumNotes 46
In Memoriam: Dwight L. Clements, Warren E. Van Norman, Peter R. Durland, Tedd C. Huston, Frederick J. Hurlbut, Leslie W. Jensen, Marshall D. Becker, Claire D. Johnson, Glenn A. Rodehorst, Dick H. Hartsock, Walter E. Gallawa, Gordon M. “Mac” Hull, Richard C. Schmoker, Glen J. Soukup, James L. Birkel, Marilyn A. Hutchinson, Stephen A. Mazurak, Richard L. Davenport, Frank E. Landis, John L. Erickson, James E. Gordon, Wayne C. Kreuscher, M. Susan Carlson, George G. Rhodes, Kirk O. Kuwitzky, Thomas R. Dilley, Jane P. Nerison, Royce E. Norman, Larry D. Ohs, Deborah K. Gilg, James B. Fleming, Jonathan C. Hook, Wayne L. Blankenbiler, Douglas F. Fagan, Jone M. Bosworth, Joe E. Schaefer, David G. Brown, Gregory P. Bolton, Shane E. Perkins, Matthew G. Marvin 54
Multicultural Legal Society celebrates 50th anniversary 59
Nebraska Law Review commemorates centennial anniversary 60
Gould named Law Alumni Master 61
Schmid Law Library undergoes transformation 62
College welcomes new faculty 66
Report on Giving 6
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