11 research outputs found
"Test me and treat me" - attitudes to vitamin D deficiency and supplementation: a qualitative study
No full text© 2015 BMJ Open, "Test me and treat me"-attitudes to vitamin D deficiency and supplementation: a qualitative study. This manuscript version is made available under the Creative Commons Attribution Licens
Mapping and Visualizing Research of COVID-19 with Immunology: A Bibliometric Study
No full textThis paper aims to demonstrate the World’s scientific contribution to COVID-19 and Immunology research for 2020–2022 and the emerging research pattern using various bibliometric parameters. The research publications were
retrieved from the SCOPUS database and analyzed using MS Excel, R Studio, and VOS Viewer software. The VOS Viewer software is mainly used for networking and visualization to understand the research pattern better. A total of 2877 documents were retrieved from SCOPUS using the search topic
COVID-19 and Immunology. The research found that DIAO B was a prolific author in COVID-19 and Immunology research, with 1247 publications. The most productive countries and institutions in this field were the USA (555 publications) and Huazhong university of science and technology (409
publications). The five hot author keywords are COVID-19, Sars-cov-2, Vaccine, Coronavirus and Antibody. The present study provides various networking map of research publications regarding the immune response
during COVID-19 infection. The results benefit researchers and practitioners in India and worldwide for understanding the pattern of research on COVID19 and identifying the potential immune response against SARS-CoV-
A Scientometric Exploration of Global Publications of Yoga Research from 2002-2021
No full textThe paper aims to provide an in-depth evaluation of the research output of Yoga for a period of 20 years, from 2002-2021. A total of 3498 documents retrieved from the Web of Science(WoS) database are considered for this analysis. The research finds that Cramer, H. was a most prolific author in Yoga scientific research with the highest 79 publications and top 2980 global citations. The most productive country is the USA, with 23967 publications, followed by India (5822) and Australia (2258). There was a significant increase in publications during the period of study. Most articles were published in the Journal of Alternative and Complementary Medicine. The highest number of Yoga research papers were contributed by two Indian institutions: All India Institute of Medical Sciences and the National Institute of Mental Health and Neuro-Sciences. Cramer H’ published the highest number of papers. The present study will be helpful for other researchers for further studies on Yoga research and policymakers for funding decisions and strategies
Genus-Level Identification of Bladder-Resident Bacteria Associated with Recurrent Urinary Tract Infection in Post-Menopausal Women by Fluorescence In-Situ Hybridization
No full textThe 60th Annual Medical Student Research Forum at UT Southwestern Medical Center (Tuesday, February 1, 2022, 3-6 p.m., Microsoft Teams)Each year the Medical Student Research Program awards students for the best oral presentation and the best poster presentation as judged by faculty across campus. This author received an award as one of the best poster presentations at this forum.INTRODUCTION/BACKGROUND: Antibiotic-recalcitrant recurrent urinary tract infection (rUTI) is common in postmenopausal women. Many women elect to undergo electrofulguration (EF) of areas of chronic cystitis when standard antibiotic therapies fail. One potential benefit of this procedure is the removal of tissue-resident bacterial communities.1 Although tissue-resident bacteria have been observed in the bladder walls of postmenopausal women with rUTI by fluorescence in situ hybridization (FISH), genus and species level identification of these bacteria by FISH has not yet been reported.2 Here, we use genus-specific probes to quantify Escherichia spp. present in the bladder biopsies taken from postmenopausal women with rUTI undergoing EF and investigate the relationship between detected bacterial community sizes and stage of cystitis.
METHODS: Following IRB approval, bladder biopsies were obtained from consenting postmenopausal women who elected EF for the advanced management of rUTI. Biopsies were immediately fixed in paraformaldehyde and then paraffin-embedded and sectioned (5 μm). FISH was performed as described previously2 using the following probe sets: 1. Scramble-AlexaFluor488/647 (control), 2. Universal 16s rRNA-AlexaFluor647 (all bacteria), and 3. Escherichia 16s rRNA-AlexaFluor488. Slides were imaged using a Zeiss LSM880 with a 63x objective. 10 randomly sampled images were collected for each biopsy section. Images analysis was performed blinded to quantify bacterial community sizes. Patients were classified by cystitis stage (Stage 1 (trigone alone) to 4 (pancystitis)) determined by cystoscopy at time of the biopsy. Least mean squares statistical analysis was used to generate an average number of bacterial organisms per 10x1 μm2 for each cystitis stage.
RESULTS: Genera-specific FISH was performed on bladder biopsies from 23 women. The universal 16s rRNA probe detected tissue-resident bacteria in the biopsies of 95.7% (22/23) women. Tissue-resident Escherichia spp. were detected in the bladder biopsies of 52.1% (12/23) women. The highest average bacterial community sizes were observed in Stage 1 cystitis bladder biopsies (8.4 per 10x1 μm2, 95% C.I. 6.6 - 10.1).
CONCLUSION: For the first time, 16s rRNA FISH was used to detect Escherichia spp. in the bladders of postmenopausal women electing to undergo EF for the advanced management of rUTI. Interestingly, bladder-resident bacterial community sizes were highest in bladder biopsies from women with Stage 1 cystitis.Southwestern Medical Foundatio
Citation Patterns followed in Research Papers of the DESIDOC Journal of Library & Information Technology
No full textThe paper presents Citation patterns of 6914 citations appearing in 399 papers published in DESIDOC Journal of Library & Information Technology from 2012 to 2018. The researcher identified in the paper were Volume-wise Analysis of Citations per Article, authorship pattern, forms of literature, Chronological Analysis of Citations and a ranked list of top 25 cited Library Science journals. The most cited author of DESIDOC Journal of Library & Information Technology was Gupta B.M. The study finds that highest number of citations was single-authored (51.59 %), and 6.72 % citations were from books and only 55.07% citations were from journals. Chronological distribution of cited reference has been analyzed that the maximum number of citations are covered during the period of 2001-2010 i.e. 55647 (38.18 %)
PANC Study (Pancreatitis: A National Cohort Study):national cohort study examining the first 30 days from presentation of acute pancreatitis in the UK
No full textBackgroundAcute pancreatitis is a common, yet complex, emergency surgical presentation. Multiple guidelines exist and management can vary significantly. The aim of this first UK, multicentre, prospective cohort study was to assess the variation in management of acute pancreatitis to guide resource planning and optimize treatment.MethodsAll patients aged greater than or equal to 18 years presenting with acute pancreatitis, as per the Atlanta criteria, from March to April 2021 were eligible for inclusion and followed up for 30 days. Anonymized data were uploaded to a secure electronic database in line with local governance approvals.ResultsA total of 113 hospitals contributed data on 2580 patients, with an equal sex distribution and a mean age of 57 years. The aetiology was gallstones in 50.6 per cent, with idiopathic the next most common (22.4 per cent). In addition to the 7.6 per cent with a diagnosis of chronic pancreatitis, 20.1 per cent of patients had a previous episode of acute pancreatitis. One in 20 patients were classed as having severe pancreatitis, as per the Atlanta criteria. The overall mortality rate was 2.3 per cent at 30 days, but rose to one in three in the severe group. Predictors of death included male sex, increased age, and frailty; previous acute pancreatitis and gallstones as aetiologies were protective. Smoking status and body mass index did not affect death.ConclusionMost patients presenting with acute pancreatitis have a mild, self-limiting disease. Rates of patients with idiopathic pancreatitis are high. Recurrent attacks of pancreatitis are common, but are likely to have reduced risk of death on subsequent admissions.</div
The Tiger Genome and Comparative Analysis with Lion and Snow Leopard Genomes
No full textTigers and their close relatives (Panthera) are some of the world’s most endangered species. Here we report the de novo assembly of an Amur tiger whole-genome sequence as well as the genomic sequences of a white Bengal tiger, African lion, white African lion and snow leopard. Through comparative genetic analyses of these genomes, we find genetic signatures that may reflect molecular adaptations consistent with the big cats’ hypercarnivorous diet and muscle strength. We report a snow leopard-specific genetic determinant in EGLN1 (Met39\u3eLys39), which is likely to be associated with adaptation to high altitude. We also detect a TYR260G\u3eA mutation likely responsible for the white lion coat colour. Tiger and cat genomes show similar repeat composition and an appreciably conserved synteny. Genomic data from the five big cats provide an invaluable resource for resolving easily identifiable phenotypes evident in very close, but distinct, species
PANC Study (Pancreatitis: A National Cohort Study): national cohort study examining the first 30 days from presentation of acute pancreatitis in the UK
No full textAbstract
Background
Acute pancreatitis is a common, yet complex, emergency surgical presentation. Multiple guidelines exist and management can vary significantly. The aim of this first UK, multicentre, prospective cohort study was to assess the variation in management of acute pancreatitis to guide resource planning and optimize treatment.
Methods
All patients aged greater than or equal to 18 years presenting with acute pancreatitis, as per the Atlanta criteria, from March to April 2021 were eligible for inclusion and followed up for 30 days. Anonymized data were uploaded to a secure electronic database in line with local governance approvals.
Results
A total of 113 hospitals contributed data on 2580 patients, with an equal sex distribution and a mean age of 57 years. The aetiology was gallstones in 50.6 per cent, with idiopathic the next most common (22.4 per cent). In addition to the 7.6 per cent with a diagnosis of chronic pancreatitis, 20.1 per cent of patients had a previous episode of acute pancreatitis. One in 20 patients were classed as having severe pancreatitis, as per the Atlanta criteria. The overall mortality rate was 2.3 per cent at 30 days, but rose to one in three in the severe group. Predictors of death included male sex, increased age, and frailty; previous acute pancreatitis and gallstones as aetiologies were protective. Smoking status and body mass index did not affect death.
Conclusion
Most patients presenting with acute pancreatitis have a mild, self-limiting disease. Rates of patients with idiopathic pancreatitis are high. Recurrent attacks of pancreatitis are common, but are likely to have reduced risk of death on subsequent admissions.
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Determinants of morbidity and mortality following emergency abdominal surgery in children in low-income and middle-income countries
No full textBackground Child health is a key priority on the global health agenda, yet the provision of essential and emergency surgery in children is patchy in resource-poor regions. This study was aimed to determine the mortality risk for emergency abdominal paediatric surgery in low-income countries globally.
Methods Multicentre, international, prospective, cohort study. Self-selected surgical units performing emergency abdominal surgery submitted prespecified data for consecutive children aged <16 years during a 2-week period between July and December 2014. The United Nation's Human Development Index (HDI) was used to stratify countries. The main outcome measure was 30-day postoperative mortality, analysed by multilevel logistic regression.
Results This study included 1409 patients from 253 centres in 43 countries; 282 children were under 2 years of age. Among them, 265 (18.8%) were from low-HDI, 450 (31.9%) from middle-HDI and 694 (49.3%) from high-HDI countries. The most common operations performed were appendectomy, small bowel resection, pyloromyotomy and correction of intussusception. After adjustment for patient and hospital risk factors, child mortality at 30 days was significantly higher in low-HDI (adjusted OR 7.14 (95% CI 2.52 to 20.23), p<0.001) and middle-HDI (4.42 (1.44 to 13.56), p=0.009) countries compared with high-HDI countries, translating to 40 excess deaths per 1000 procedures performed.
Conclusions Adjusted mortality in children following emergency abdominal surgery may be as high as 7 times greater in low-HDI and middle-HDI countries compared with high-HDI countries. Effective provision of emergency essential surgery should be a key priority for global child health agendas
Timing of nasogastric tube insertion and the risk of postoperative pneumonia: an international, prospective cohort study
No full textAim: Aspiration is a common cause of pneumonia in patients with postoperative ileus. Insertion of a nasogastric tube (NGT) is often performed, but this can be distressing. The aim of this study was to determine whether the timing of NGT insertion after surgery (before versus after vomiting) was associated with reduced rates of pneumonia in patients undergoing elective colorectal surgery. Method: This was a preplanned secondary analysis of a multicentre, prospective cohort study. Patients undergoing elective colorectal surgery between January 2018 and April 2018 were eligible. Those receiving a NGT were divided into three groups, based on the timing of the insertion: routine NGT (inserted at the time of surgery), prophylactic NGT (inserted after surgery but before vomiting) and reactive NGT (inserted after surgery and after vomiting). The primary outcome was the development of pneumonia within 30 days of surgery, which was compared between the prophylactic and reactive NGT groups using multivariable regression analysis. Results: A total of 4715 patients were included in the analysis and 1536 (32.6%) received a NGT. These were classified as routine in 926 (60.3%), reactive in 461 (30.0%) and prophylactic in 149 (9.7%). Two hundred patients (4.2%) developed pneumonia (no NGT 2.7%; routine NGT 5.2%; reactive NGT 10.6%; prophylactic NGT 11.4%). After adjustment for confounding factors, no significant difference in pneumonia rates was detected between the prophylactic and reactive NGT groups (odds ratio 1.03, 95% CI 0.56–1.87, P = 0.932). Conclusion: In patients who required the insertion of a NGT after surgery, prophylactic insertion was not associated with fewer cases of pneumonia within 30 days of surgery compared with reactive insertion
