159 research outputs found
Chips & Tips: Rapid prototyping of a PMMA microfluidic chip with integrated platinum electrodes
No full textThis is a very quick and useful method for researchers who do not have access to high tech micro-fabrication facilities and want to try out an idea or a test in a quick, cheap and simple fashion. In this case it is for those of us who want to test certain techniques such as in-channel electrochemical, conductivity or impedance measurements. It also saves time and costs from using high tech fabrication techniques and will aid the researcher in future designs that can then be fabricated the more conventional way in a clean room. In addition, it is a cheap and effective way of introducing undergraduate and masters students to various chip technique
Quim Monzó and Contemporary Catalan Culture (1975–2018): Cultural Normalization, Postmodernism and National Politics
No full textQuim Monzó (born Barcelona, 1952) is considered Catalonia’s most influential contemporary author, his work studied as a catalyst for the modernization of Catalan culture after General Franco’s death in 1975. Analysing Quim Monzó’s trajectory from countercultural artist in 1970s Barcelona to celebrity intellectual in the present day, Colom-Montero argues that Monzó’s work encapsulates many of the cultural, aesthetic and political tensions in post-Francoist Catalonia. Offering first-time English-language analyses of Monzó’s multifaceted artistic trajectory (including political cartoons, translations, journalistic writing, media collaborations and social media persona) as well as new close readings of some of his better-known literary texts, Colom-Montero maps the paradigmatic cultural shifts that have characterized the transition from late Francoist to autonomous and post-referendum Catalonia. At a time of deepening divisions between Catalonia and Spain, in this book Monzó emerges as an author and public intellectual aiming to build a Catalan politico-cultural sphere different from and opposed to that of Spain
Capillary electrophoresis characterisation of a rapid prototyped PMMA chip for particle analysis
No full textMàster en Nanociència i NanotecnologiaA rapid and cheap method has been developed for the
fabrication of a capillary electrophoresis chip for the
preliminary characterization of particles. The microfluidic
chips were fabricated using polymethyl methacrylate
(PMMA) with integrated platinum electrodes without the
need of using high technology microfabrication techniques.
The chips were characterized using electroosmotic flow
(EOF) with different channel treatments. The electrodes
were characterised with impedance and conductivity
measurements using both static and electrophoretic flow,
respectively. Nine micron diameter particles were detected
and their electrophoretic mobility determined using
capillary electrophoresis and conductivity detection
Mechanism for Vipp1 spiral formation, ring biogenesis, and membrane repair
No full textThe ESCRT-III-like protein Vipp1 couples filament polymerization with membrane remodeling. It assembles planar sheets as well as 3D rings and helical polymers, all implicated in mitigating plastid-associated membrane stress. The architecture of Vipp1 planar sheets and helical polymers remains unknown, as do the geometric changes required to transition between polymeric forms. Here we show how cyanobacterial Vipp1 assembles into morphologically-related sheets and spirals on membranes in vitro. The spirals converge to form a central ring similar to those described in membrane budding. Cryo-EM structures of helical filaments reveal a close geometric relationship between Vipp1 helical and planar lattices. Moreover, the helical structures reveal how filaments twist—a process required for Vipp1, and likely other ESCRT-III filaments, to transition between planar and 3D architectures. Overall, our results provide a molecular model for Vipp1 ring biogenesis and a mechanism for Vipp1 membrane stabilization and repair, with implications for other ESCRT-III systems
La nación como relato
No full textEl trabajo explora la evolución y las paradojas de la construcción narrativa de la nación. Centrándose en los ejemplos históricos, el autor nos muestra las principales teorías de la narrativa de la identidad y, al mismo tiempo, aquellos vinculados con el problema ideológico de la construcción de las naciones en la modernidad. //// “The nation as narration” (Francisco Colom González). The work explored the developments and paradoxes of the narrative construction of the nation. Focusing in historical examples, the author shows us the principal theories of the identity narrative and, at the same time, he linked them with the ideological problem of the construction of the nations in the modernity
From eye lens cells to lens membrane proteins : Development and application of a hybrid high-speed atomic force microscopy/optical microscopy setup
No full textJe utilise le AFM et le HS-AFM pour étudier les caractéristiques mécaniques du cellule du cristallin et aussi des protéines de membrane de la cellule, AQP0 et Connexon. L’énergie d'interaction de la AQP0 est -2.7 kBT, très nécessaire pour former les microdomaines de jonctions (junctional microdomain). Aussi c' est la première fois qu il est possible de voir des protéines individuel et son mouvement en cellules vivants. La formation de microdomaines est important pour la transparence du cristallin, et le AQP1 ne le peux faire.I used the AFM and HS-AFM for characterise the eye lens and the eye lens membrane protein, AQP0 and connexon.A QP0-AQP0 interaction energy is -2.7kBT, it is important for the formation of junctional microdomains, which keep the distance between the cells lens and lens transparency. this is the first report which is present time the visualization of unlabelled membrane proteins on living cells under physiological conditions. AQP1 can not maintain the lens transparency because it does not form junctional microdomains
Mechanism for Vipp1 spiral formation, ring biogenesis and membrane repair
No full textHere, we collect an image dataset of Vipp1 polymerization dynamics on supported lipid bilayers using atomic force microscopy (AFM). A JPK NanoWizard Ultraspeed AFM (Bruker and JPK BioAFM) equipped with USC-F0.3-k0.3-10 cantilevers with spring constant of 0.3N nm−1, resonance frequency of about 300 kHz (Nanoworld), was employed for image acquisition. The AFM was operated in tapping mode with a cantilever oscillation frequency near to 150kHz. Both topographic and phase images were analysed using JPKSPM Data Processing, ImageJ, and WSxM software.Peer reviewe
Interfacial Stresses on Droplet Interface Bilayers Using Two Photon Fluorescence Lifetime Imaging Microscopy
No full textResponse of lipid bilayers to external mechanical stimuli is an active area
of research with implications for fundamental and synthetic cell biology.
However, there is a lack of tools for systematically imposing mechanical
strains and non-invasively mapping out interfacial (membrane) stress
distributions on lipid bilayers. In this article, we report a miniature
platform to manipulate model cell membranes in the form of droplet interface
bilayers (DIBs), and non-invasively measure spatio-temporally resolved
interfacial stresses using two photon fluorescence lifetime imaging of an
interfacially active molecular flipper (Flipper-TR). We established the
effectiveness of the developed framework by investigating interfacial stresses
accompanying three key processes associated with DIBs: thin film drainage
between lipid monolayer coated droplets, bilayer formation, and bilayer
separation. Interestingly, the measurements also revealed fundamental aspects
of DIBs including the existence of a radially decaying interfacial stress
distribution post bilayer formation, and the simultaneous build up and decay of
stress respectively at the bilayer corner and center during bilayer separation.
Finally, utilizing interfacial rheology measurements and MD simulations, we
also reveal that the tested molecular flipper is sensitive to membrane fluidity
that changes with interfacial stress - expanding the scientific understanding
of how molecular motors sense stress.Comment: 8 pages, 4 figure
La gestión de la diversidad etnocultural: ¿Qué modelo de integración?
No full textEjemplar dedicado a: Políticas para el siglo XXIRecensiones sobre la obra:
-Teresa Chapa Brunet, "La Prehistoria española a examen". ABC literario, 6 enero 1990: XII.
-M. Angeles Querol, Trabajos de Prehistoria 47, 1990: 395-397.
-J.J. Storch de Gracia, Historia 16, XV, 167, 1990: 125.
-Paloma González, Robert W. Chapman, Antonio Gilman, "Entorn a...MªI. Martínez Navarrete, Una reflexión crítica sobre la Prehistoria española: la Edad del Bronce como paradigma (Madrid, 1989)". Revista d´Arqueologia de Ponent, 2, 1992: 261-266.[EN] Studying ethnicity has traditionally been a difficult matter. The reason is the dynamic an changing nature of social identities in general. This paper traces the intellectual origins of such a difficulty in the history of social sciences and the relevance of the idea of contingency for an ethical approach to ethnicity. After bringing into consideration the main models of modern citizenship, the author analyzes the position of immigrants in its normative structure and the possibilities open in Spanish society for a pluralist model of social integration and the democratic management of interethnic relations.[ES] El estudio de a etnicidad ha presentado tradicionalmente una serie de dificultades. La razón última de ello estriba en la naturaleza dinámica y difusa de las identidades sociales. Este artículo reconstruye el origen intelectual de esas dificultades y la importancia de la idea de contingencia para una consideración normativa de la etnicidad. Tras repasar los principales modelos de la ciudadanía moderna, el texto analiza el papel de los inmigrantes en la estructura de derechos cívicos y concluye con una reflexión sobre las posibilidades abiertas en la sociedad española para un modelo pluralista de integración y una gestión democrática de las relaciones interétnicas.Peer reviewe
Charles de Villers; sein Leben und seine Schriften. Ein Beitrag zur Geschichte der geistigen Beziebungen zwischen Deutschland und Frankreich.
No full textLettre à Mademoiselle D.S. was formerly ascribed to Issac de Colom Duclos.Includes bibliography.Mode of access: Internet
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