118 research outputs found
DOĞU ANADOLU BÖLGESİ'NDE TEDAVİ AMAÇLI KULLANILAN BAZI ALLIUM TÜRLERİNİN BİYOLOJİK AKTİVİTE VE FİTOKİMYASAL KOMPOZİSYONU
No full textBitkisel kökenli doğal ürünler zengin bir biyoçeşitlilik, lokal flora ve faunaya sahip olan Doğu Anadolu Bölgesi’nde kanser, karın ağrısı, diyabet vb. hastalıkların tedavisi ve/veya önlenmesinde etkin bir şekilde kullanılmaktadır (Dalar ve Mukemre, 2018)
Plant Taxa Used in the Treatment of Diabetes in Van Province, Turkey
No full textDespite
the richness of local flora and medicinal plant utilization, there is no any
report on documentation of antidiabetic botanicals used in Van province.
Therefore, the present study aimed to record accumulation of the traditional
antidiabetic medicinal plants of Van province in order to preserve the valuable
local medicines knowledge, which has been threatened by urbanization. Antidiabetic folk
medicines were determined via field surveys conducted in 1065 settlements
during the period of 2014-2017 through medicinal plants questionnaire
and structured face-to-face interviews (600 informants) with local people who
are well known in the province for their long practice in traditional medicine.
69 plant taxa (35 species with undocumented antidiabetic medicinal use in
scientific literature for Turkey) including five endemic and one rare to Turkey
belong to 16 families (principally Asteraceae and Lamiaceae) were recorded for
their traditional antidiabetic use. 52 different vernacular names were detected
which were mainly indicate morphological characteristics. Infusion prepared
from leaf and flower organs were found as the most common preparation method of
local medicines in the province. Use value analysis showed that Rheum ribes, Urtica dioica, Scutelleria orientalis
subsp. pichleri, Diplotenia
cachrydifolia, Teucrium polium, Rosa canina, Campanula glomerata subsp. hispida, Rumex scutatus, Helichrysum
plicatum subsp. plicatum and Tanacetum balsamita subsp. balsamita might serve promising
pharmaceutical agents for diabetes treatment
Design and applications of electrochemical biosensors for the detection of plant extract-DNA interactions
No full textBitki özlerindeki bileşikler ve DNA arasındaki olası etkileşimin ve hatta DNA hasarının doğrudan tespiti için bu projede kullanılıp atılabilir özelliklere sahip (tek kullanımlık) elektrokimyasal DNA biyosensörü geliştirildi. Projede buzağı timus bezi çift sarmallı DNA (dsDNA) ve sentetik prob/hedef DNA dizileriyle Sempervivum davisii Muirhead, Plantago anatolica Tutel & R. Mill. ve Verbascum cheiranthifolium Boiss. var. cheiranthifolium Boiss. bitki özlerinin etkileşimleri, kalem grafit elektrot (KGE) kullanılarak guanin ve ekstrakt oksidasyon sinyallerine dayanarak elektrokimyasal olarak diferansiyel puls voltametri (DPV) tekniğiyle incelenmiştir. Her üç bitki özünün de DNA ile etkileştiği saptanmıştır. Ekstrakt konsantrasyonu ve ektrakt-DNA etkileşim süresi gibi deneysel parametrelerdeki değişiklikler, hem guanin hem de bitki özü elektroaktivitesindeki yanıt farkları kullanılarak incelenmiştir. Elektrokimyasal yöntemlerin DNA ve DNA hedefli maddeler arasındaki etkileşimlere uygulanması araştırılmıştır. Elde edilen sonuçlar, DNA hasarına neden olan bileşiklerin analizinde pratik uygulamalar için de ümit vericidir.In this Project, single use electrochemical DNA biosensors were developed for direct detection of possible interactions between DNA and compounds in plant extracts. The calf thymus double-stranded DNA (dsDNA) and synthetic probe / target DNA sequences were interacted with plant extracts of Sempervivum davisii Muirhead, Plantago anatolica Tutel & R. Mill with. and Verbascum cheiranthifolium Boiss. var. Cheiranthifolium. The interactions of plant extracts were investigated by differential pulse voltammetry (DPV) technique based on guanine and extract oxidation signals by using pencil graphite electrode (KGE). All three plant extracts were found to interact with DNA. Changes in experimental parameters, such as extract concentration or time to extract-DNA interactions, were investigated using response differences in both guanine and plant extract electroactivity. Additionally, the application of electrochemical methods for the detection of interactions between DNA and DNA-targeted any kind of substances was investigated and discussed. The obtained results are also promising for practical applications in the analysis of compounds that cause DNA damage
ABIOTIC STRESS FACTORS AND SIGNAL MOLECULES AFFECT SECONDARY METABOLITES COMPOSITON OF ST. JOHN’S WORT BOTH IN VIVO AND IN VITRO
No full textABIOTIC STRESSFACTORS AND SIGNAL MOLECULES AFFECT SECONDARY METABOLITES COMPOSITON OF ST.JOHN’S WORT BOTH IN VIVO AND IN VITROİN VİVO VE İN VİTRO ŞARTLARDA YETİŞTİRİLEN SARIKANTARON BİTKİSİNDE ABİYOTİK STRES FAKTÖRLERİ VE SİNYAL MOLEKÜLLERİNİN SEKONDERMETABOLİT KOMPOZİSYONUNA ETKİLERİ Neşe ERAY VURANRes. Asist., Van Yüzüncü Yıl University, Faculty of Science, Departmentof Molecular Biology and Genetic, [email protected] (CorrespondingAuthor), Orcid ID: 0000-0001-6387-1493 Abdullah DALARAssoc. Prof. Dr., Van Yüzüncü Yıl University, Faculty of Pharmacy, [email protected], OrcidID: 0000-0002-0080-2519 Musa TÜRKERProf. Dr., Yildiz Technical University, Faculty ofEngineering, Department of Bioengineering, [email protected],Orcid ID: 0000-0003-3195-1119 Inthe present study, Hypericum perforatumL. was regenerated in vitro and abioticstress factors and signal molecules were applied on in vitro regenerants toinvestigate the change of phenolic compounds concentration comparing to plantscollected from field. The effects of media composition, explant type and PlantGrowth Regulators (PGRs) in different combination and concentration on callusproduction, plant regeneration and secondary metabolite production wereevaluated. The quantitative analysis of phenolic composition was performed byHigh Performance Liquid Chromatography (HPLC). Accordingto the results, MS medium supplemented with 1 mg/L 2, 4-D + 1 mg/L BA wasdetermined to be the best medium for callus formation in dark. When micro propagationis considered, MS medium supplemented with 2 mg/L NAA + 1 mg/L BA was the best PGRscombination. Multiple shoots developed in MS supplemented with 2 mg/L NAA + 1mg/L BA were separated from each other and rooted in hormone-free MS medium. Theleaf explant gave the highest shoot number. The shoot explant produced thehighest amount of callus. Theeffects of abiotic stress factors such as drought and UV-C and some signalmolecules such as ascorbic acid, salicylic acid, sucrose and different PGRs onthe production of phenolic compounds were investigated.It was demonstrated that callus was able to producehypericin and pseudohypericin compounds. B5 and SH media gave better resultsthan MS medium for the production of hypericin and pseudohypericin. Thecombination of NAA + BA was detected the best PGRs on phenolic compoundsproduction. Chlorogenic acid concentrationwas increased by UV and sucrose, quercetin was increased by ascorbic acid andpseudohypericin was increasedby drought applications compared to the control. The increases in the amounts of phenolic compounds werestatistically significant (p <0.01). Rutin was not detected in the plantsgrown in vivo and in vitro. However, the plant grown inlaboratory conditions produced rutin compound under drought stress. Keywords:Abiotic stress, Hypericum perforatumL., Phenolic compound, Signal Molecule, Tissue culture</p
Phenolic composition and enzyme inhibitory activities of Eryngium bornmuelleri (Tusî) from Eastern Anatolia
No full text
Phenolic contents, antioxidant capacities and inhibitory activities against key metabolic syndrome relevant enzymes of herbal teas from Eastern Anatolia
No full textSix traditional herbal infusions from Eastern Anatolia region of Turkey were investigated for their potential to suppress metabolic syndrome. Hydrophilic lyophilized extracts representing herbal infusions exhibited comparable or superior levels of phenolic compounds and antioxidant capacity [ferric reducing antioxidant power (FRAP assay) and oxygen radical absorbance capacity (ORAC assay)] to commercially available black tea (Camellia sinensis) and numerous herbal infusions. All teas successfully suppressed the activities of key enzymes involved in metabolic syndrome: alpha-amylase, alpha-glucosidase, pancreatic lipase and angiotensin converting enzyme (ACE). Phlomis armeniaca, Salvia limbata and Plantago lanceolata exhibited weak inhibitory activities against alpha-amylase and pronounced inhibitory activities against alpha-glucosidase, which suggests potential anti-diabetic properties. Moreover, P. lanceolata exhibited pronounced inhibitory activity against pancreatic lipase. This study suggests potential anti-diabetic and anti-obesity activity of P. lanceolata herbal infusion, and supports its traditional use as anti-diabetic tea. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved
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