48 research outputs found
Web GIS solution and visualization towards sustainability of world heritage site / Abd. Manan Samad, Mat Salam Karnadi and Azman Mohd Suldi
No full textE-government is a promotion from the government side to encourage local authority using of new technologies for better operation. Web GIS is the one system which occupies e-government condition for geography information data sharing within organization and outside world. Sharing Information by using Web GIS by local authorities will probably improve performance inside organization to be more efficient, and could develop positive relationship with visitor and the public. Good Information presentation for spatial and non spatial information will facilitate relationship with public and also easy to produces decision for every development and planning which operates. This is easy system and can be increased internally and external inside each local council. Web based GIS is one of the systems who can used by local council in department of town planning for each local council to achieve objective inside of contents the agenda 21 produce in Earth Summit at Rio De Janeiro's conference in June 1992. Agenda 21 have produce are goals for Local Authority to implement negotiation process with society to formulate Local Agenda 21 respectively. So to deliver that meaning local authority must having equipment to channel information such as Geography’s information environmental area inside cater to societal involvement, private sector and powerful to cooperative the authorities inside planning and manage their environmental area direction to sustainable development. In the local council party for department of town planning it must have a Web based GIS system to channel information to civilians, private sector and local authority to be doing something planning result and development inside administration area by quick and accurate
Potential of tissue cultured medicinal plants in Malaysia
No full textMedicinal plants possess many secondary products that exhibit biological activities such as antioxidant, anticancer, anti-inflammatory, antibacterial and anti microbial. Scientific findings have demonstrated that tissue culture techniques could be an alternative tool to propagate plant in vitro and manipulate secondary metabolites in medicinal plants. This review aims to give an update on the various plant regeneration of some locally used medicinal plants in Malaysia such as Eurycome longifolia Jack, Zingiber officinale Roscoe, Centella asiatica L., Justicia gendarussa Burm. f, Kaempferia galanga L. and Orthosiphon stamineus Benth. Different type of cultures including organ, callus and cell cultures is also discussed
Isolation and Identification of NIF Gene Homologue of Locally Isolated Azorhizobium Strains from Stem Nodules of Sesbania Rostrata
No full textCharacterisation of UPMR 43, UPMR 44 and X was conducted.
Morphological studies of colonies showed that all strains gave a relatively large
gummy, watery, translucent or whitely opaque colour on a modified glutamate agar.
Intrinsic antibiotic resistance (IAR) patterns of all strains indicated that they were
resistant to ampicillin of up to 50 mg/l but susceptible to streptomycin, tetracycline
and kanamycin at concentrations of 25, 15 and 10 mg/l, respectively. The growth
characteristics of UPMR.43 and UP"MR.44 (in a modified glutamate medium (GM) and peptone medium (PA)) was studied using shake flask method. UPMR43 grew
better in GM compared to PA but the reverse was true for UPMR44. PA
significantly reduced the cell concentration and affected growth characteristic. The
generation times for both strains in both media were less than 6 h indicating that
they were fast growers. The banding pattern of total DNA of three strains digested
with EcoRI showed that they were very similar.
A large plasmid, subject to confirmation was detected in UPMR44 using
Hirsch method and one small plasmid in UPMR43 using Casse method. A 13.5 kb
BamHI and a 7.0 kb EcoRI DNA fragment from total DNA extracts of all local
strains were detected in Southern blot experiments using probes carrying the
nifHDK genes of Klebsiella pneumoniae and Azotobacter vinelandii. The results
showed that the nif genes of the Azorhizobium strains were detectable using the
heterologous nif probes from K. pneumoniae and A. vinelandii. The similar results
obtained from colony morphology, generation time, IAR patterns, total DNA
restriction patterns and DNA-DNA hybridisation of the three strains studied suggests
that they may be the same strain. The obtained results also showed that local strains
have a similar characteristics with A. caulinodans ORS571. Strain X may be
considered a unique strain since S. rostrata was easily infected although it is not
native to Malaysia. It is postulated that this strain may have an alternative host plant
Tissue culture, transformation and secondary products in members of the 'Asteraceae'
No full textEThOS - Electronic Theses Online ServiceGBUnited Kingdo
In vitro propagation and flavonoid contents in local justicia gendarussa Burm. F
No full textJusticia gendarussa or locally known as Gandarussa have been reported to contain naringenin and kaempferol that possess biological activities such as strong antioxidant and cytotoxicity. This paper present plant regeneration studies of J. gendarussa using different type of explants (young leaf and nodal segment) and media (B5 or MS basal media) supplemented with different concentration of plant growth regulators. The results indicated a maximum of callus fresh weight gains was obtained (1.21 g 0.11) when young leaf explants were cultured on MS medium supplemented with Kn (3.0 mgL -1) + 2,4-D (1.0 mgL -1). For shoot induction, high percentage of shoot regeneration (100 %) and number of shoots produced per explants (5.33 0.33) was recorded when nodal explants were cultured on MSB5 plates supplemented with 2.0 mgL -1 BAP. Then, the shooted explants were rooted in medium-free hormone. Determination of flavonoids (naringenin and kaempferol) content in methanolic organ extracts of in vitro plantlets was analyzed using Gas Chromatography-Flame Ionization Detector [GC-FID]. Green calli (701.27 mgkg -1 ± 7.69) contained significant higher naringenin content than in vitro leaves (419.99 mgkg -1 ± 133.39). However, there was no significant difference in kaempferol content between green calli (214.19 mgkg -1 ± 8.28) and in vitro leaves (242.03 mgkg -1 ± 41.13). In conclusion, accumulation of flavonoids in vitro plants offers potential of manipulating culture parameters such as medium composition and hormone concentration for enhancement of flavonoids content in plant tissues
An audit of the referral letter received at Klinik Pakar Orthodontik Jln Abd Samad
No full textThe objectives of this study were to determine the prevalence and the pattern of
hypodontia among orthodontic patients in Klinik Pergigian Jalan Sultan Mahmud, Kuala Terengganu. (Copied from article)
Isolation of high quality RNA from plant rich in flavonoids, Melastoma decemfidum Roxb ex. Jack.
No full textChalcone synthase (CHS) is a plant-specific enzyme that synthesises naringenin chalcone, an essential precursor of the flavonoid biosynthetic pathway. Naringenin and kaempferol are two flavonoids that have been demonstrated to inhibit the proliferation of HeLa cells. To study chalcone synthase gene regulation in Melastoma decemfidum, we developed a high-yield total RNA isolation method to assemble a partial putative CHS cDNA sequence. Our results indicated that a modified CTAB method produced the highest total RNA yield (8.26±3.99 μg/gFW) compared to other methods. Thus, we used this method to isolate total RNA from different types of tissues from this plant. Our improved protocol produced high-quality total RNA from different tissues, including the mature leaf (7.02±2.60 μg/gFW), stem (4.27±1.72 μg/gFW), flower bud (37.54±10.61 μg/gFW), flower (21.31±5.20 μg/gFW), and root (3.38±1.89 μg/gFW). The total RNA was then converted into cDNA, and a putative CHS gene product (~1049 bp fragment) was amplified using degenerate primers. A partial CHS gene sequence shared 80% homology with an Anthurium andraeanum CHS gene sequence (AY232492) and 92% homology with the amino acid sequence of the Acer maximowiczianum CHS gene (AEK80412.1), as determined using BlastN and BlastX, respectively. This study shows that our modified CTAB method allows for the isolation of high-quality and high-yield total RNA from various tissues of M. decemfidum. A partial putative CHS gene was amplified,thus confirming that the modified CTAB method is suitable for RT-PCR and gene isolation
Successful plant regeneration of Orthosiphon stamineus from petiole
No full textWe present a successful regeneration of Orthosiphon stamineus plant from petiole. All of the explants were cultured on Murashige and Skoog (MS)-based medium supplemented with various concentrations of benzylaminopurine (BAP) and naphthalene acetic acid (NAA) at the growth condition (25 ± 2°C, 16-h photo period at light intensity of 22.85 μmol /m2 /s) for 8 weeks. Results showed that petiole explants cultured at combination of 1.0 mg/L BAP and 0.2 mg/L NAA gave highest number of shoot produced per explants (4.33 ± 0.33) and a maximum callus fresh weight gains (3.2 ± 1.9 g) as compared to other treatments. The first shoot was observed from petiole cultured at all treatments in 4 weeks culture except for 0.1 mg/L BAP + 0.5 mg/L NAA treatment. For root induction, treatment at 0.1 mg/L NAA produced the highest number of roots produced per shoot (9.8 ± 3.1) compared to other treatments. Regeneration comparison between petiole, young leaf and stem segments showed that petiole is the most suitable explant for an efficient plant regeneration system of O. stamineus
