13 research outputs found
Adaptive Evolution and Addressing the Relevance for Genetic Improvement of Sago Palm Commodity
No full textPrinsip Dasar Teknik Kultur Jaringan
No full textTeknik Kultur jaringan Tanaman merupakan salah satu teknik pengembangbiakan tanaman yang dianggap masih relatif baru, meskipun sebenarnya sejak tahun 1883 teori dasar yang merupakan pondasi melakukan kultur jaringan atau teori totipotensi sel telah dinyatakan oleh Sachwann dan Schleiden.
Teori totipotensi sel menyebutkan setiap sel dapat bereplikasi secara otomatis dan dapat diregenerasikan membentuk tanaman lengkap. Kultur jaringan itu sendiri pertama kali dicobakan pada tahun 1902 oleh Haberlandt.
Buku ini ditujukan untuk memudahkan mahasiswa mempelajari dan memahami teori teknik kultur jaringan dalam waktu yang singkat. Setelah selesai mempelajari tulisan ini diharapkan para mahasiswa cukup mendapat latar belakang ilmu pengetahuan kultur jaringan beserta terminologinya sehingga mahasiswa diharapkan mengerti teori dan terampil melakukan kultur jaringan.
Buku ini merupakan perpaduan berbagai buku teks book dan pengalaman penelitian penulis.
Selamat mempelajari
Pertumbuhan dan Perkembangan Biakan Semai Jamur Sagu (Volvariella sp.) Membentuk Tubuh Buah Pada Berbagai Macam Media Tumbuh
No full textSago mushroom (Volvariella sp.) was known as edible mushroom, which taste delicious and use as protein sources of local people in Papua. Sago mushroom has high nutrition contents, high prices, and broadly market opportunity. The objectives of research were investigated growth and development of inoculum hyphae on various growing medium. The growth and development medium used in this experiment was called M1, M2, M3, M4, and M5 formulation medium.� The results of experiment showed that all of the five formulation mediums were supported of growth abundant hyphae.� Growth and development of hyphae to form pinhead, egg, elongation, and maturity stages were observed at M1, M2, and M4 formulation medium, but not at M3 and M5 formulation medium.� M5 formulation medium just supported of growing hyphae and M3 formulation medium supported of growth and development hyphae become pinhead stages but not developed to further stages.</jats:p
Genetic Variation of Sago Palm (Metroxylonsagu Rottb.) Progenies with Natural Pollination by Using RAPD Markers
No full text
Genetic Relationship of Sago Palm (Metroxylon sagu Rottb.) in Indonesia Based on RAPD Markers
No full textThe areas of sago palm (Metroxylon sagu Rottb.) forest and cultivation in the world were estimated two million hectares and predicted 50 % of that areas located in Indonesia. Distribution of sago palm areas in Indonesia is not evenly distributed as well as their diversities. Information of plant genetic diversities and genetic relationship is very important to be used for germ plasm collection and conservation. The objectives of research were revealed the genetic relationships of sago palm in Indonesia based on RAPD molecular markers. Fragments amplification PCR products were separated on 1.7 % agarose gel, fixation in Ethidium Bromide, and visualized by using Densitograph. Genetic relationships of sago palm in Indonesia showed that sample in individual level were inclined mixed among the other and just formed three groups. Genetic relationship of sago palm population showed that samples populations from Jayapura, Serui, Sorong, Pontianak, and Selat Panjang were closely related each others based on phylogenetic analysis and formed clustered in one group, event though inclined to be formed two subgroups. Populations from Manokwari, Bogor, Ambon and Palopo were closed related each others, they were in one group. Genetic relationships in the level of island were showed sago palm from Papua, Kalimantan, and Sumatra closely related. Sago palms from Maluku were closed related with sago palm from Sulawesi whereas sago palm from Jawa separated from the others. Based on this observation we proposed that Papua as centre o
Kloning dan karakterisasi daerah promoter gen penyandi ADP glucose pyrophosphorylase dari Metroxylon sagu rendemen pati-tinggi dan -rendah [Cloning and characterization of promoter region of ADP glucose pyrophosphorylase-encoding gene from Metroxylon sagu with high- and low-starch content]
No full textADP-glucose pyrophosphorylase (AGPase) is one of the key enzymes in the starch biosynthesis. In many plants, the activity of this enzyme was reported to affect the yield and composition of the produced starch. This research is a part of an effort to develop molecular markers for early selection of high starch-yielding of sago palm. The purpose of the research was to isolate promoters of AGP gene and to analyze the differences in their DNA sequences between sago palm with high starch content (MsHS) and low starch content (MsLS). DNA was isolated and purified from the leaves of the two sago palm. The promoter region of AGP was amplified by Genome Walking technique. The specific primers were designed by Primer3 program based on the information of DNA sequence of AGP genes of sago palm from previous studies. Selected DNA fragments resulted from Genome Walking were isolated from the gel, cloned into E. coli, and analyzed its DNA sequence. DNA sequence analysis showed that one DNA fragment from MsHS (± 1500 bp) and one DNA fragment from MsLS (> 2000 bp) were confirmed as a 5’ upstream of the AGP gene. Further in silico analysis using MEME program identified various DNA motifs of cis-acting elements, which confirmed that those DNA fragment were promoter region of the gene. Preliminary analysis showed the differences in DNA sequences and motives of cis-acting elements in the promoter region of the two samples which might influence or indirectly associated with the character of the starch yield in sago palm.</jats:p
